Predicted mutation
evidence seq id position mutation freq annotation gene description
JC AE015451 303,121 +GCT 40.3% coding (1286/1314 nt) envZ → Osmolarity sensor protein EnvZ

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451 = 30312117 (0.460)12 (0.310)
+GCT		 10/308 NT 40.3% coding (1286/1314 nt) envZ Osmolarity sensor protein EnvZ
?AE015451 303122 = 18 (0.450)coding (1287/1314 nt) envZ Osmolarity sensor protein EnvZ

TGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/302903‑303121
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTC  >  AE015451/303122‑303294
                                                                                                                                                                                                                           |||                                                                                                                                                                             
TGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTG                                                                                                                                                                        <  2:607998/229‑1
                              CGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCG                                                                                                                                                                  <  2:396133/205‑1
                                                                                  GATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCT                                                                                >  1:601430/1‑235
                                                                                     CGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATGGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCT                                                                                                                                                                      <  2:15063/146‑1
                                                                                                                                                         CGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGG                                                               >  1:52057/1‑181
                                                                                                                                                               CGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCCTTCTTCTC  >  1:367442/1‑236
                                                                                                                                                                           TAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACC                                                                                                                 <  1:283075/113‑1
                                                                                                                                                                           TAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACC                                                                                                                 >  2:283074/1‑113
                                                                                                                                                                                   CTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGC                                                                         >  1:556603/1‑145
                                                                                                                                                                                   CTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGC                                                                         <  2:556602/145‑1
                                                                                                                                                                                      GCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACC                                                                                                                 <  1:163589/102‑1
                                                                                                                                                                                      GCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACC                                                                                                                 >  2:163589/1‑102
                                                                                                                                                                                                                       TGGGGCTGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACCAGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAcg                                                                     >  2:56296/1‑111
                                                                                                                                                                                                                           |||                                                                                                                                                                             
TGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/302903‑303121
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCTTCACGGCTAAAGCCGCACCTACAGGGATTGCGCAGTACCTGTGGCAGCGGCTTGGCCGCGAAGCGGCGGGTAGAGGCTTGAATCAGCCTTTGCCCTTGGTCCGGGTCTGATTGGGCCCGCCATTCTTCTC  >  AE015451/303122‑303294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.