Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	5,201,568	Δ98 bp	44.0%	intergenic (‑54/‑29)	PP_4582 ← / → PP_4583	conserved membrane protein of unknown function/putative Peptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5201567	22 (0.550)	12 (0.310)	12/330	NT	44.0%	intergenic (‑53/‑127)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase
	?	AE015451	5201666 =	9 (0.230)					intergenic (‑152/‑28)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase

CGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5201355‑5201567 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCG  >  AE015451/5201666‑5201862                                                                                                                                                                                                                                                                                                                                                                                                                            CGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGAC                                                                                                                                                                                >  1:417583/1‑236   ACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCA                                                                                                                                                                                                <  1:378749/218‑1     GATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGAC                                                                                                                                                                                <  2:511986/232‑1         GTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCA                                                                                                                                                                           <  2:417582/233‑1                               CCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGGACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCCGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCG                                                                                                                                                   >  1:693010/1‑235                                                        CAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCAACCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGG                                                                                                                                                                                                  <  1:14439/163‑1                                                                                               CAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCG                                                                                                                                      <  2:410817/184‑1                                                                                                                            ACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACG                                                        >  2:694411/1‑233                                                                                                                                     AGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTCCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACGCTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCA                                                                                                                             <  2:132846/155‑1                                                                                                                                       GCGAAGGTGTAGTCGTTCTCGGGCATGGTGTTCGTGCACGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCT                                                               <  1:169887/215‑1                                                                                                                                                             TCATGGTGTTCGTGCAAGACAGGCAAAGAGATGCCGATGATCAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTA                                                                                                                   <  2:598761/141‑1                                                                                                                                                                                CAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCG  <  1:694412/235‑1                                                                                                                                                                                CAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGC         >  1:321020/1‑228                                                                                                                                                                                                              tcCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCC                                                                                                                                       <  2:110930/70‑1                                                                                                                                                                                                               cCCGGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGC                                                                    <  1:113876/137‑1                                                                                                                                                                                                                  GGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATG                                                                                                                 <  2:280950/90‑1                                                                                                                                                                                                                                                                                                                                                                                                                            CGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5201355‑5201567 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCCCGAGGTACTCGCTTACCTGCAAGCTGAAAACGCCTACCAGGAAGCCTGCCTGGCCGACCAGGCACCGTTGCGTGAGCGCCTGTTCG  >  AE015451/5201666‑5201862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.