Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	1,780,067	Δ62 bp	12.7%	intergenic (+26/+19)	PP_1587 → / ← dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1780066	37 (0.930)	5 (0.130)	4/322	NT	12.7%	intergenic (+25/+81)	PP_1587/dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative
	?	AE015451	1780129 =	33 (0.860)					intergenic (+88/+18)	PP_1587/dapC	NhaP‑type Na+/H+ and K+/H+ antiporters/N‑succinyl‑L,L‑diaminopimelate aminotransferase alternative

GCTGGAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGC  >  AE015451/1779834‑1780297                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          gCTGGAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCG                                                                                                                                                                                                                                           >  2:526644‑M1/1‑233 (MQ=255)      aaaGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGccc                                                                                                                                                                                                                                      <  2:383814/233‑1 (MQ=255)      aaaGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGcg                                                                                                                                                                                                                                   <  1:732107/236‑2 (MQ=255)                    aGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGccc                                                                                                                                                                                                                                      <  2:657885/219‑1 (MQ=255)                     gTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgcgc                                                                                                                                                                                                                                        <  2:665219/216‑1 (MQ=255)                     gTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTActgcg                                                                                                                                                                                                                  <  1:526645/236‑1 (MQ=255)                         tGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgcgc                                                                                                                                                                                                                                        >  1:211588/1‑212 (MQ=255)                               cAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGa                                                                                                                                                                                                        <  1:187873/236‑1 (MQ=255)                                   gCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGt                                                                                                                                                                                                                        >  1:519500/1‑216 (MQ=255)                                                  aTGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTActgcg                                                                                                                                                                                                                  <  1:2044/207‑1 (MQ=255)                                                    ggACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATACGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgcgc                                                                                                                                                                                                                                        <  2:238890/185‑1 (MQ=255)                                                      aCTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                     <  1:403701/216‑1 (MQ=255)                                                       cTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTAc                                                                                                                                                                                                                      >  2:443912/1‑198 (MQ=255)                                                          gCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                     >  1:6762/1‑212 (MQ=255)                                                             ggAGCGGCGTTTACGCTTGCGTGCCCTGAGG‑‑GCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGc                                                                                                                                                                        >  1:664108/1‑236 (MQ=255)                                                                  ggcgTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCa                                                                                                                                                                                                   <  1:563889/206‑1 (MQ=255)                                                                                       tGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTActgcg                                                                                                                                                                                                                  <  2:6762/170‑1 (MQ=255)                                                                                            gcgcAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCa                                                                                                                                           <  1:334955/236‑1 (MQ=255)                                                                                                  cGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgcgc                                                                                                                                                                                                                                        <  2:169302/139‑1 (MQ=255)                                                                                                  cGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAg                                                                                                                                      >  1:386439/1‑235 (MQ=255)                                                                                                                cAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGccc                                                                                                                                                                                                                                      <  2:43479/127‑1 (MQ=255)                                                                                                                      gCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCa                                                                                                                                                                                                   <  2:263660/154‑1 (MQ=255)                                                                                                                      gCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCa                                                                                                                                                                                                   >  1:263661/1‑154 (MQ=255)                                                                                                                          cGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgcg                                                                                                                                                                                                                                         >  2:653847/1‑114 (MQ=255)                                                                                                                           gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTg                                                                                                                                             >  2:313783/1‑203 (MQ=255)                                                                                                                           gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGa                                                                                                             >  1:519949/1‑235 (MQ=255)                                                                                                                                   cTGGTGGGGGATGAGGTGGTGGGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCgg                                                                                                    <  1:330952/236‑1 (MQ=255)                                                                                                                                    tggtggGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTAGCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGa                                                                                                                                                                                                        <  2:15664/135‑1 (MQ=255)                                                                                                                                     ggtgggtgATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGACCCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGGAGGGGTTGATCAGCGGTTTTGCAGGa                                                                                                                                        <  1:197643/198‑1 (MQ=255)                                                                                                                                        gggggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTAcc                                                                                                                                                                                         <  1:640838/146‑1 (MQ=255)                                                                                                                                                           ccaGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGACAt                                                                                                                                                                                                     <  2:183617/113‑1 (MQ=255)                                                                                                                                                            aaGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggc                                                                                                                               <  1:64787‑M1/186‑109 (MQ=255)                                                                                                                                                              gTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGCAGGGGTTGCTCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCGTCGATGCATTCGGCCAGCGGt                                                                                            >  2:244451/1‑217 (MQ=255)                                                                                                                                                                        aGCTGGATAGGAGTGAGGCGATCCTGGGTTCAGTCAAGTATACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAg                                                                                                                                                     <  1:417499/150‑1 (MQ=255)                                                                                                                                                                            ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgc                                                                                                                                                                                                                                          >  1:631365/1‑63 (MQ=255)                                                                                                                                                                            ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGgcgc                                                                                                                                                                                                                                          <  2:631364/63‑1 (MQ=255)                                                                                                                                                                            ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccat                                                                                                                                              >  2:294885‑M1/1‑62 (MQ=255)                                                                                                                                                                            ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCAcg                                                                       >  2:257466/1‑224 (MQ=255)                                                                                                                                                                            ggATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCAcc                                                            >  1:679388/1‑235 (MQ=255)                                                                                                                                                                                 ggagggaggCGAGCCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGc                                                                                               <  2:664205/194‑1 (MQ=255)                                                                                                                                                                                       agGCGAACCTGGGCTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTc                                                                                                                                                                                                                                  >  2:145245/1‑60 (MQ=255)                                                                                                                                                                                          cGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTc                                                                                                                        >  2:320354/1‑161 (MQ=255)                                                                                                                                                                                          cGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCa                                                                         >  2:347023/1‑208 (MQ=255)                                                                                                                                                                                                tGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTc                                                                                                               >  1:317567/1‑164 (MQ=255)                                                                                                                                                                                                tGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAg                                                                                                >  1:148944/1‑179 (MQ=255)                                                                                                                                                                                                  ggTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacat                                                                    <  1:60078‑M1/207‑168 (MQ=255)                                                                                                                                                                                                                    cTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                     <  2:87446/58‑1 (MQ=255)                                                                                                                                                                                                                    cTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                     >  1:87446/1‑58 (MQ=255)                                                                                                                                                                                                                      aTCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtac                   >  1:721368‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                      aTCAAGGCCCTTCGCGGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtac                   >  1:313474‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                      aTCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAg                              >  1:6620/1‑223 (MQ=255)                                                                                                                                                                                                                        cAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                     >  2:193146/1‑54 (MQ=255)                                                                                                                                                                                                                        cAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                                                                                                     <  1:193146/54‑1 (MQ=255)                                                                                                                                                                                                                                cgggcgggcGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaacgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggta                                                                                <  1:653848‑M1/162‑156 (MQ=255)                                                                                                                                                                                                                                   gcgGGCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggcccacagg      <  1:443913‑M1/236‑230 (MQ=255)                                                                                                                                                                                                                                     gggCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgaca                                                                                               >  2:281678‑M1/1‑5 (MQ=255)                                                                                                                                                                                                                                     gggCGCGCCCGCTCCCACACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTa       >  2:642593/1‑233 (MQ=255)                                                                                                                                                                                                                                     gggCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCAcgcg                                  >  1:605135/1‑204 (MQ=255)                                                                                                                                                                                                                                     gggCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTAc      >  2:635624/1‑232 (MQ=255)                                                                                                                                                                                                                                      ggCGtgcccgcgaaggggtggatcagcggttttgcaggaaggcgcgaatgcgcgccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggc             <  1:115265‑M1/226‑223 (MQ=255)                                                                                                                                                                                                                                       gCGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggcccacaggtaga  >  1:432702‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                        cGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggtgaactcggcatcgtcgcccggtaccttggcccacagg      >  2:717663‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                        cGtgcccgcgaaggggttgatcagcggttttgcaggaaggcgcgaatgcgctccgccgcttcgatgcattcggccagcggtgcaaccagtgccatgcgcacgcgcccggcacccgggttcaccccatccacttcacgcgacaggtacgaccccggtaccacggttacatgctgggcttcgaacaggtcgcgggg                                           >  2:115265‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          GCTGGAAAGCTACGACGACAGTGCTGAAGCCAAAGCCCTGGCCGCACAGATGGACTTGCTGGAGCGGCGTTTACGCTTGCGTGCCCTGAGGGCGCAGCGGTTGGAGCTGTACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTATCAAGGCCCTTCGCGGGCGCGCCCGCTCC‑‑CACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCGCACGCGCCCGGCACCCGGGTTCACCCCATCCACTTCACGCGACAGGTACGACCCCGGTACCACGGTTACATGC  >  AE015451/1779834‑1780297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.