New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	5926821 =	33 (0.830)	4 (0.110)	4/320	NT	11.2%	coding (1074/1623 nt)	PP_5195	putative Iron ABC transporter, permease protein
	?	AE015451	5926859 =	32 (0.840)					coding (1036/1623 nt)	PP_5195	putative Iron ABC transporter, permease protein

AGGGCGGATGCGCTCCAGGCTGCTCTCCAGCGGCCCGTAGGCCACCGCGATGAAGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAGCGCAGGCGAACACCAGCAGGCACCAGCCACTGGC  >  AE015451/5926587‑5927048                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      aGGGCGGATGCGCTCCAGGCTGCTCTCCAGCGGCCCGTAGGCCACCGCGATGAAGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTAGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAAccg                                                                                                                                                                                                                                      >  2:457562/1‑235 (MQ=255)       aggcgctgtatcacctgcgcggcctcaaggcgctgttggccagtggctggtgcctgctggtgttcgcctgcgctttcgtcatcccgctgctgcagttgctggcgtggttctggcaacgcggccggcatgacctggatgagcgttatatcggcctggtgctgcacaccctgtacctgggcagcatggcggcgctggtcacggtatgcgtggcgctgctgctggcctttgCCCGgcg‑c                                                                                                                                                                                                                               <  1:457563‑M1/8‑1 (MQ=255)              tCCAGGCTGCTCTCCAGCGGCCCGTAGGCCACCGCGATGAAGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATAcc                                                                                                                                                                                                                       <  1:699704/236‑1 (MQ=255)                               cGGCCCGTAGGCCACCGCGATGAAGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGTTCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCt                                                                                                                                                                                                         >  2:408918/1‑233 (MQ=255)                                                 gATGAAGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGAACGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGt                                                                                                                                                                                                             >  1:210671/1‑211 (MQ=255)                                                      aGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCcagcagca                                                                                                                                                                                 >  1:514477/1‑234 (MQ=255)                                                      aGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCagcagcagc                                                                                                                                                                               >  1:231017/1‑236 (MQ=255)                                                          cACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGcc                                                                                                                                                                            <  1:408919/235‑1 (MQ=255)                                                                gTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCa                                                                                                                                                                       >  1:695967/1‑234 (MQ=255)                                                                                       ggCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CCAGGCACCGACGGGCGGTATC‑CGCGCCGGGCAAAGGCCAGCAGCAgcg                                                                                                                                                                              >  2:711989/1‑204 (MQ=255)                                                                                        gCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCg                                                                                                                                                                                                                                    <  2:10950/151‑1 (MQ=255)                                                                                                 cagcagcGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTAc                                                                                                                                     <  1:118094/235‑1 (MQ=255)                                                                                                         gCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGACGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACGCCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GAGACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCCGCAGCGCCa                                                                                                                                                                           >  2:430683/1‑189 (MQ=255)                                                                                                                 gcgcCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCa                                                                                                                                                   <  2:646484/205‑1 (MQ=255)                                                                                                                     cgccCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATCCCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCcagcagcag                                                                                                                                                                                <  1:256930/172‑1 (MQ=255)                                                                                                                     cgccCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCACCCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTgg                                                                                                                                                                                                       >  2:152277/1‑149 (MQ=255)                                                                                                                                   gACAACGGGATGGCCAGGTGGGTGGCGAGGTAACTCAAGGCCAGCATGATCGATCCCGCCAACACCGACCCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTgg                                                                                                                                                                                                       <  2:175810/135‑1 (MQ=255)                                                                                                                                   gACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTc                                                                                                     <  2:323815/233‑1 (MQ=255)                                                                                                                                                 cAGGTGGTTGTCGAGGTAACGGAAGGCCAGCATGAGCGATACCGCCACCACCGAACCGGGCAGGGCATACCCCAGATGGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCa                                                                                                                     <  2:115829/203‑1 (MQ=255)                                                                                                                                                         tGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGt                                                                               <  2:425895/233‑1 (MQ=255)                                                                                                                                                            cGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCAt                                                                                                                                                                      >  2:251085/1‑143 (MQ=255)                                                                                                                                                             gAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGAG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCa                                                                                                                                          <  2:412663/170‑1 (MQ=255)                                                                                                                                                                   aCTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCGGGTCATGCCGGCCGCGTTGCCAGGAcctgt                                                                  >  1:187897/1‑233 (MQ=255)                                                                                                                                                                     tGAAGGCCAGCATGATCGATACCGCCACCACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCAtgct                                                                                                                                               <  2:255742/157‑1 (MQ=255)                                                                                                                                                                     tGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGcc                                                                <  1:743499/236‑1 (MQ=255)                                                                                                                                                                                    tCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCGCCA‑GGCACCGACGGGCGGTATC‑CGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCa                                                                                                                     <  2:741836/168‑1 (MQ=255)                                                                                                                                                                                           cGCCACCCCCGAGCCGGGCAGGGCATACCCCAGATGGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATgccggc                                                                                    <  2:187897/194‑1 (MQ=255)                                                                                                                                                                                                    cGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGa                                                                                                               <  2:115306/158‑1 (MQ=255)                                                                                                                                                                                                         cGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATGCCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAgg                                                                                                                                        <  2:210671/128‑1 (MQ=255)                                                                                                                                                                                                         agggcagggcaTACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAGCGCAGGc                             <  1:606170/234‑1 (MQ=255)                                                                                                                                                                                                              ggtatgcgtggcgctgctgctggcctttgCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCa                                                                                                                                                   >  1:648946‑M1/30‑112 (MQ=255)                                                                                                                                                                                                                  tgcgtggcgctgctgctggcctttgCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAg                                                                                                                                                           <  1:409646‑M1/75‑1 (MQ=255)                                                                                                                                                                                                                   gcgtggcgctgctgctggcctttgCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGa                                                                                                               <  1:601077‑M1/119‑1 (MQ=255)                                                                                                                                                                                                                       gccAGATTGGCCCGGCCAACACCGGCG‑CG‑GATACCGCCCGTCGGCGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGAtata                                                                                                           <  2:182603/142‑1 (MQ=255)                                                                                                                                                                                                                         cAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAg                                    >  2:606169/1‑212 (MQ=255)                                                                                                                                                                                                                           ctgctgctggcctttgCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCgccgcc                                                                                                                                                    <  2:648945‑M1/82‑1 (MQ=255)                                                                                                                                                                                                                             ttGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAGCGCAGGCGAACACCAGCAGGCACCAGc         >  1:302102/1‑235 (MQ=255)                                                                                                                                                                                                                               ggccaggccaACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAg                                    <  2:354416/206‑1 (MQ=255)                                                                                                                                                                                                                                   aggccaACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTg                                                        >  2:23604/1‑182 (MQ=255)                                                                                                                                                                                                                                   aggccaACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAGCGCAGGCGAACACCAGAAGGCACCAGCCACTGGc  >  1:16868/1‑236 (MQ=255)                                                                                                                                                                                                                                        aaCCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCg                                                                                                                >  2:339147/1‑121 (MQ=255)                                                                                                                                                                                                                                          ccccGGCG‑CG‑GATGCCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCCTACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAAcc                                                                     <  1:148355/162‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      AGGGCGGATGCGCTCCAGGCTGCTCTCCAGCGGCCCGTAGGCCACCGCGATGAAGCGCACCAGGTAGGCCAGCAGCAACGCCGAGAGGCTACCTAGCAGCAGCGGCTTGCCGGCGCCGCCCAGCCAACTGGACAACGGGATGACCAGGTGGTTGTCGAGGTAACTGAAGGCCAGCATGATCGATACCGCCAACACCGAACCGGGCAGGGCATACCCCAGATTGGCCAGGCCAACCCCGGCG‑CG‑GATACCGCCCGTCGGTGCCTGGCGCCGGGCAAAGGCCAGCAGCAGCGCCACGCATACCGTGACCAGCGCCGCCATGCTGCCCAGGTACAGGGTGTGCAGCACCAGGCCGATATAACGCTCATCCAGGTCATGCCGGCCGCGTTGCCAGAACCACGCCAGCAACTGCAGCAGCGGGATGACGAAAGCGCAGGCGAACACCAGCAGGCACCAGCCACTGGC  >  AE015451/5926587‑5927048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.