New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4022305	22 (0.550)	5 (0.130)	4/316	NT	19.0%	coding (1/942 nt)	PP_3547	putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
	?	AE015451	4022806 =	22 (0.590)					intergenic (‑501/+167)	PP_3547/emrB	putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/multidrug efflux transport system ‑ permease subunit

TATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCACACCAGCAGA  >  AE015451/4022602‑4023039                                                                                                                                                                                                              |                                                                                                                                                                                                                                          cgttctccatgggtttgccaatggtttggggaagggaacatcgacaataggcgaaatcgcgctgcgttggaatgacgaacatcagcacagccaaccctctgcttttgatctggcttttgatctggcttttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatGGATGCCCGTCACCTCCCTTCGCAAGCCCt                                                                                                                                                                                                              >  1:698251‑M2/206‑235 (MQ=255)              aGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCg                                                                                                                                                                                                                      <  1:329653/213‑1 (MQ=255)              aGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCg                                                                                                                                                                                                                      >  2:329652/1‑213 (MQ=255)                         aGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGc                                                                                                                                                                                                                     >  2:583820/1‑203 (MQ=255)                               gaagggaacatcgacaataggcgaaatcgcgctgcgttggaatgacgaacatcagcacagccaaccctctgcttttgatctggcttttgatctggcttttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCcg                                                                                                                                                                                  >  2:569741‑M2/176‑233 (MQ=255)                                      cccTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCg                                                                                                                                                                                         >  1:394915/1‑218 (MQ=255)                                           ttttttggTTACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTa                                                                                                                                                                    <  2:381138/228‑1 (MQ=255)                                              gcggGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATcgc                                                                                                                                                                >  1:387293/1‑235 (MQ=255)                                                    cACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGCGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCg                                                                                                                                                                                                                      >  2:391439/1‑175 (MQ=255)                                                                   gcgACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCAcc                                                                                                                                                                                                                              <  1:387849/152‑1 (MQ=255)                                                                              aacatcagcacagccaaccctctgcttttgatctggcttttgatctggcttttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTTGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCg                                                                                                                                      >  1:366842‑M2/129‑230 (MQ=255)                                                                              aacatcagcacagccaaccctctgcttttgatctggcttttgatctggcttttgatcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagc                                                                                                                                  >  1:365839‑M2/129‑234 (MQ=255)                                                                                  cAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCCAGCCCTGCGCTCGGcctccc                                                                                                                                                                                                >  2:237386/1‑166 (MQ=255)                                                                                        cgctgcgCTCCCATCATCCGGCCCCTGCGCTGCGCTCCGGGGTTCCCTCACTCCGGCCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagc                                                                                                                      >  1:240631/7‑235 (MQ=255)                                                                                          gCTGGCTCCTATCATCCGGGCCCTGCGCTACGCGCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGt                                                                                                                                                                                           <  1:614255/164‑1 (MQ=255)                                                                                               cTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCCCGGCCTCCTGAAGTCGCa                                                                                                                                                                                       >  1:158452/1‑163 (MQ=25)                                                                                                               ccTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcgagagc                                                                                                          >  2:588834/1‑224 (MQ=255)                                                                                                                cTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcgagagcgagagcga                                                                                                        >  1:328318/1‑225 (MQ=255)                                                                                                                      tACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcg                                                                                                                           >  2:382594/1‑200 (MQ=255)                                                                                                                             cGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGc                                                                                 <  1:732343/235‑1 (MQ=255)                                                                                                                                ggTTCCCTCACTCCGGCCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCa                                                                                             >  1:730637/1‑220 (MQ=255)                                                                                                                                     tcttgatcttgatcttgatcttcgcgacttcaggaggccgaacgcaggccttgcggaggcaggtgacgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagagcga                                                                                                                    >  2:595459‑M2/74‑193 (MQ=255)                                                                                                                                        cGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCACAAGCCGAagcg                                                                                                                                 >  2:4686/1‑176 (MQ=255)                                                                                                                                               ggCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGCGGCC‑CAGCGCTTGCCGGGCCTCCCTGCCCGTCACCTCCCTTCGCAAGCCCTGCGCCCGGCCTCCTGAAGTCGCCATCCGCGGCGCCt                                                                                                                                                                          >  2:49689/1‑128 (MQ=11)                                                                                                                                                                       cgaaGGCCCCATCCTGGGGCCTCAGCGCTTGACGGGCATGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCt                                        >  1:580114/4‑235 (MQ=255)                                                                                                                                                                            gCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGc                                    >  2:640746/1‑233 (MQ=255)                                                                                                                                                                            gCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCg                                   >  2:292593/1‑234 (MQ=255)                                                                                                                                                                                               gcgcTTGACGGGCATGGATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCg                 >  1:522360/1‑234 (MQ=255)                                                                                                                                                                                                cgcTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCa                                                                                             >  1:576272/1‑157 (MQ=255)                                                                                                                                                                                                 gcTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTg                                                                                                                                                                         >  2:76714/1‑80 (MQ=255)                                                                                                                                                                                                 gcTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTACCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCACGCCTTAGCGGTAAAGGGCGGCTTGGCGaa               >  2:396573/1‑234 (MQ=255)                                                                                                                                                                                                 gcTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGAGGCGCCTGGGcg                                                                                                                                                                     >  1:3170/1‑83 (MQ=255)                                                                                                                                                                                                      acgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCacac         >  1:364147‑M2/9‑235 (MQ=255)                                                                                                                                                                                                       cgggcatCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCACACca       >  1:121916‑M2/8‑236 (MQ=255)                                                                                                                                                                                                            atCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGACTTGGCGAACCACACCAGCAGa  >  1:405897‑M2/3‑236 (MQ=255)                                                                                                                                                                                                              ccATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagag                                                                                                                       <  1:321294/117‑1 (MQ=255)                                                                                                                                                                                                              ccATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAagcgagagcgagag                                                                                                                       >  2:321293/1‑117 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                          TATTCATTCACGCAGGTGGCGCATAGTCACCCTTCCGCCCTTACGGCGGGTCACTTTTTGTCAAACGCGACAAAAAGTAACCAAAAAACGCTGGCTCCTATCATCCGGCCCCTGCGCTACGCTCCGGGGTTCCCTCGCTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCCTGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTTCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCCGCGGCGCCTGGGCTATCGCGCGCTTAGAAGCAAAATCAAAAGCCGAAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCGAGAGCATCGGGATCTTGCCTTGATTTTAGCTCAATGCCCCGCCGATGCCGGGCCCGCCTTAGCGGTAAAGGGCGGCTTGGCGAACCACACCAGCAGA  >  AE015451/4022602‑4023039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.