Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	4,980,585	+GGC	100%	intergenic (‑24/+44)	PP_4387 ← / ← flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4980585	0 (0.000)	25 (0.640) +GGC	23/330	NT	100%	intergenic (‑24/+44)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE
	?	AE015451	4980586 =	0 (0.000)					intergenic (‑25/+43)	PP_4387/flgE	conserved exported protein of unknown function/Flagellar hook protein FlgE

GCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCAC  >  AE015451/4980358‑4980817                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        agggcttgctcaatgggcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGc                                                                                                                                                                                                                                  <  1:301957‑M2/8‑1 (MQ=255)                 gcgcggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAac                                                                                                                                                                                                                  <  1:257624‑M2/24‑1 (MQ=255)                     ggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaccaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACaa                                                                                                                                                                                                                    >  2:726882‑M2/209‑230 (MQ=255)                                          ggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGt                                                                                                                                                                                         <  1:580083‑M2/49‑1 (MQ=255)                                                agtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcc                                                                                                                                                                                                                                         >  1:94596‑M2/182‑182 (MQ=255)                                                                    cccgggttgtgggccgaacacagggagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGt                                                                                                                                                                                         <  1:240745‑M2/49‑1 (MQ=255)                                                                                                           aggctttgccattgatagaacggggcgggggcagcgggggcaggcagagccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAAt                                                                                                                                                                                                      <  1:142621‑M2/36‑1 (MQ=255)                                                                                                                       tgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaccaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCt                                                                                                            <  1:726883‑M2/126‑1 (MQ=255)                                                                                                                                  ggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGCtt                                                                                                  >  2:341819‑M2/100‑235 (MQ=255)                                                                                                                                                  tgtataagagacagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCa                                                                                 <  1:46712‑M2/153‑1 (MQ=255)                                                                                                                                                    aggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGctc                                                                                                                   >  1:661626‑M2/82‑200 (MQ=255)                                                                                                                                                          aaccagcaccagcaaggggaacaaggccaggaacgacagccgcttcattcgcgggaaccttcacattcttggggcCGCTTTGCgg                                                                                                                                                                                                                                >  2:103410‑M2/76‑85 (MQ=255)                                                                                                                                                                  ccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGc                                                                                                                     >  2:559832‑M2/68‑184 (MQ=255)                                                                                                                                                                   cagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAgg                                                                                                    >  1:118993‑M2/67‑200 (MQ=255)                                                                                                                                                                              acaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGt                                                                                   >  2:46712‑M2/56‑206 (MQ=255)                                                                                                                                                                                    ccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCGTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCTTGATGGTGCttt                                                                                                                                                         >  2:727827‑M2/50‑130 (MQ=255)                                                                                                                                                                                        taacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAAt                                            >  1:203239‑M2/46‑235 (MQ=255)                                                                                                                                                                                          acgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAATTGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTAc                                                                                               >  2:706612‑M2/44‑182 (MQ=255)                                                                                                                                                                                          acgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGt                                                                              >  2:750473‑M2/44‑199 (MQ=255)                                                                                                                                                                                                gccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCttt                                                                                                                                                         >  1:444192‑M2/38‑118 (MQ=255)                                                                                                                                                                                                gccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCttt                                                                                                                                                         <  2:444191‑M2/81‑1 (MQ=255)                                                                                                                                                                                                    cttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAgg                                  <  2:321733‑M2/200‑1 (MQ=255)                                                                                                                                                                                                        attcgcgtgacccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGtt                                                                       <  2:94596‑M2/163‑1 (MQ=255)                                                                                                                                                                                                            gcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGGCGACGTTGGGGTCCTCCAggg                                                         >  2:199705‑M2/26‑202 (MQ=255)                                                                                                                                                                                                                    cttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTa                                                                                                <  2:418295‑M2/138‑1 (MQ=255)                                                                                                                                                                                                                    cttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTa                                                                                                >  1:418296‑M2/18‑155 (MQ=255)                                                                                                                                                                                                                                 gggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCAc  <  1:349123‑M2/232‑1 (MQ=255)                                                                                                                                                                                                                                  ggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTgg                                                                                                                                 >  2:544611‑M2/4‑108 (MQ=255)                                                                                                                                                                                                                                  ggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTgg                                                                                                                                 <  1:544612‑M2/105‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                        GCAAGGGCTTGCTCAATGGGCGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCGGAGCGCCGATCACCGGCAC  >  AE015451/4980358‑4980817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.