Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	4,847,088	A→C	11.7%	D613A (GAC→GCC)	PP_4261 →	cation‑transporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,847,088	0	A	C	11.7%	80.0 / 2.5	29	D613A (GAC→GCC)	PP_4261	cation‑transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base A (18/7);  new base C (2/2);  total (20/9)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

CGCTGGAAAACCGCTCTGAACACCCCATCGCTCGCGCCTTTGGTCGTACCGCCACACCTGCCGATGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTATCGACCAGGCCATTGGCGGCCTGCGCCCGGATGACAAACTGGACCGCCTG  >  AE015451/4846875‑4847293                                                                                                                                                                                                                      |                                                                                                                                                                                                              cgcTGGAAAACCGCTCTGAACACCCCATCGCTCGCGCCTTTGGTCGTACCGCCACACCTGCCGATGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCa                                                                                                                                                                                                  >  1:34315/1‑227 (MQ=255)                   aaCACCCCATCGCTCGCGCCTTTGGTCGTACCGCCACACCTGCCGATGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTccg                                                                                                                                                                                    >  2:569952/1‑221 (MQ=255)                      acCCCATCGCTCGCGCCTTTGGTCGTACCGCCACACCTGCCGATGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCCCCTGGCGGTCGCCCAGCAGCAGCCACTGGCCTGGt                                                                                                                                                                                        >  2:545732/1‑216 (MQ=255)                                     ccTTTGGTCGTACCGCCACACCTGCCGATGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCt                                                                                                                                                                                           >  2:744085/1‑198 (MQ=255)                                                                 tGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGAt                                                                                                                                                                             >  2:26954/1‑184 (MQ=255)                                                                                aCCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTc                                                                                                                                            >  2:575285/1‑202 (MQ=255)                                                                                aCCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTc                                                                                                                                            <  1:575286/202‑1 (MQ=255)                                                                                aCCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACCGGCCCGGCTCGGCCTg                                                                                                                                                                                >  2:132083/1‑166 (MQ=255)                                                                                aCCCGGCCTGGGCCAGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCGTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGCCCAGTGGCTGCCGCTGGGCGACCGCCAGGGGCCACTGGGCTGGTTCg                                                                                                                                                                                     >  1:471424/1‑161 (MQ=255)                                                                                               ggAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCg                                                                                              <  1:218188/233‑1 (MQ=255)                                                                                                             aCGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTgg                                                                                                                                                                                              >  2:724937/1‑123 (MQ=255)                                                                                                             aCGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGt                                                                              >  1:480956/1‑235 (MQ=255)                                                                                                                gCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGcga                                                                                                                                                               <  2:633048/151‑1 (MQ=255)                                                                                                                gCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGcga                                                                                                                                                               >  1:633049/1‑151 (MQ=255)                                                                                                                  cAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCg                                                                         >  1:338661/1‑235 (MQ=255)                                                                                                                            gcATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACGGGccgcgc                                                                                                                                                                                        <  2:303728/114‑5 (MQ=255)                                                                                                                              aTTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGCTGACCGCCt                                                                                                                                                                     >  2:161044/1‑131 (MQ=255)                                                                                                                                        cTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTATc                                                  >  1:453605/1‑236 (MQ=255)                                                                                                                                          aCCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTATCGa                                                >  1:381818/1‑236 (MQ=255)                                                                                                                                             ttCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATgg                                                                               >  2:265891/1‑202 (MQ=255)                                                                                                                                                    gcgcCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGt                                                                                                                                                                                        >  2:536367/1‑90 (MQ=255)                                                                                                                                                    gcgcCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGt                                                                                                                                                                                        <  1:536368/90‑1 (MQ=255)                                                                                                                                                                 tgCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCCCCTGGCGGTCGCCCAGCAGCAGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCggg                                                                                                <  1:509918/165‑1 (MQ=38)                                                                                                                                                                              gCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTATCGACCAGGCCATTGGCGGCCTGCGCCCGGATGAc                 >  1:102695/1‑231 (MQ=255)                                                                                                                                                                              gCTGTACCCGAGCCGCGCGGCCAGTGGCCCCTGGCGGTCGCCCAGCAGCAGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCCCGCTGCCTGCAAGGCGCGGGGCTGGcg                                                                                                                  >  2:509917/1‑133 (MQ=25)                                                                                                                                                                                    cccGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAgggg                                                                                                                                                                                                     <  2:248256/45‑1 (MQ=255)                                                                                                                                                                                           cgcgcgGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTATCGACCAGGCCATTGGCGGCCTGCGCCCGGATGACAAACTGGACCGCCTg  <  2:480955/233‑1 (MQ=255)                                                                                                                                                                                               cgGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCGCGctgcctg                                                                                                                                    >  2:194801/1‑99 (MQ=255)                                                                                                                                                                                                   cAGTGGCTGCTGCTGGGCGCCCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGGCCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGcc                                                                 <  1:132083/162‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                              CGCTGGAAAACCGCTCTGAACACCCCATCGCTCGCGCCTTTGGTCGTACCGCCACACCTGCCGATGACGTACAGAGCGTACCCGGCCTGGGCCTGGAGGGGGTGGTCGACGGCCAGCGCCTGCGCATTGGTCAGGCTACCTTCGTCTGCGCCTTGAGTGGTGCGGAAATCCCCGCTGTACCCGAGCCGCGCGGCCAGTGGCTGCTGCTGGGCGACCGCCAGGGGCCACTGGCCTGGTTCGGCCTGGATGACCGCCTGCGCGACGATGCCCCGGCCCTGCTCGCTGCCTGCAAGGCGCGGGGCTGGCACACCCTGCTGCTGTCGGGCGACAGCTCGCCGATGGTTGCCGAAGTGGCCGCGCAGCTGGGTATCGACCAGGCCATTGGCGGCCTGCGCCCGGATGACAAACTGGACCGCCTG  >  AE015451/4846875‑4847293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑