| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,964,454 | C→T | 100% | D302N (GAC→AAC) | fleQ ← | Transcriptional regulator FleQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,964,454 | 0 | C | T | 100.0% | 125.2 / NA | 33 | D302N (GAC→AAC) | fleQ | Transcriptional regulator FleQ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/14); total (19/14) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGG > AE015451/4964229‑4964666 | ggTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAg < 1:594285/236‑1 (MQ=255) aTCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGt < 1:246522/235‑1 (MQ=255) aTCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGGGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAg < 1:288094/228‑1 (MQ=255) aCCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACAAGTTCAGGCGGTAGTACAGGTTTTCGCGGACGGTGCCGTCCTCGAt > 1:49792/1‑227 (MQ=255) ggCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGa < 2:462890/218‑1 (MQ=255) cTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGc > 1:303710/1‑235 (MQ=255) tggcccgcccAGCCGTGACGACACAGCGACATGATCGAGGAGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGa < 2:189133/200‑1 (MQ=255) gTGACGACACAGCGACATGATCGAGGCGGAAGTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGa < 2:138123/188‑1 (MQ=255) gATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGc > 1:422994/1‑236 (MQ=255) aTCGAGGCGGAATTGAAGCGAATGGAACCGAGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTGCATCAGCAACGGGATGTCGTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCg < 2:224358/180‑1 (MQ=255) ggCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGAt < 2:658397/213‑1 (MQ=255) cGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGta > 2:478024/1‑177 (MQ=255) aCCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCt < 2:422993/233‑1 (MQ=255) gcTTCTCGTGCTCCAGGCACGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTgca < 1:113021/234‑1 (MQ=255) cgcgAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACgc > 1:174607/1‑225 (MQ=255) gcgAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGACGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGAc > 1:396854/1‑235 (MQ=255) gcgAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGAcc > 2:343109/1‑236 (MQ=255) tCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATc < 1:339612/236‑1 (MQ=255) gATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATg > 2:510101/1‑226 (MQ=255) tGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGt > 2:634191/1‑225 (MQ=255) cTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCt > 1:36029/1‑204 (MQ=255) cccGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAAc > 1:252584/1‑236 (MQ=255) cACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTtcgt > 2:497362/1‑222 (MQ=255) cACGCAGCGGCGCCATGTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGt < 2:203917/160‑1 (MQ=255) aCGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGt > 2:597846/1‑234 (MQ=255) ccATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGc > 1:372124/1‑235 (MQ=255) ccATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGc > 1:623829/1‑235 (MQ=255) gcGATAGGGAACACGTTCAGGCGGTAGTACTGGTTTTCGCGGAAGGTGCCGGCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCTATGCTTTGGGTCTGGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCATCACACGCAGCAGCTTGACCt < 2:237124/174‑1 (MQ=255) cGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTg > 2:508788/1‑227 (MQ=255) gAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGcc < 1:510102/236‑1 (MQ=255) cAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGc > 1:51774/1‑179 (MQ=255) ggTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTTACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAg > 2:466604/1‑187 (MQ=255) taCAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCgg > 2:546908/1‑220 (MQ=255) | GGTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGG > AE015451/4964229‑4964666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |