Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 5,087,105 A→T 12.5% L73Q (CTG→CAG)  astB ← N‑succinylarginine dihydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154515,087,1050AT12.5% 115.8 / 9.3 40L73Q (CTG→CAG) astBN‑succinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base A (10/25);  new base T (2/3);  total (12/28)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

GCCCAGCACGCGGCTGGTGGTCGGGTGCTCGATGCTGCGGTGATATTTGCAGTTGAGGTTGGCGGCGGTGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAGGCCATCAAAATTCACTTCATAGGATTTCATCGGCTAGGCTCC  >  AE015451/5086873‑5087334
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     
gcCCAGCACGCGGCTGGTGGTCGGGTGCTCGATGCTGCGGTGATATTTGCAGTTGAGGTTGGCGGCGGTGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAgcg                                                                                                                                                                                                                                    >  1:13501/1‑236 (MQ=255)
            gCTGGTGGTCGGGTGCTCGATGCTGCGGTGATATTTGCAGTTGAGGTTGGCGGCGGTGAAGTGCACGCGGCCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCTGcgcc                                                                                                                                                                                                                                  <  2:147892/226‑1 (MQ=255)
                                   cccGGTGATATTTGCAGTTGAGGTTGGCGGCGGTGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAaca                                                                                                                                                                                                 <  2:629490/234‑1 (MQ=255)
                                          atatTTGCAGTTGAGGTTGGCGGCGGGGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGcgcc                                                                                                                                                                                                                                  <  1:2345/196‑1 (MQ=255)
                                                       aGGTTGGCGGCGGTGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGt                                                                                                                                                                              >  1:300789/1‑235 (MQ=255)
                                                         gTTGGCGGCGGTGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCg                                                                                                                                                                            <  1:639255/235‑1 (MQ=255)
                                                         gTTGGCGGCGGGGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCgg                                                                                                                                                                           <  1:371774/236‑1 (MQ=255)
                                                                           cACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTc                                                                                                                                                          <  1:289177/235‑1 (MQ=255)
                                                                           cACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTc                                                                                                                                                          <  1:720891/235‑1 (MQ=255)
                                                                                                                                       gCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCct                                                                                                                  <  2:327831/215‑1 (MQ=255)
                                                                                                                                                     aaCTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCCCGCGGGGTGGACGCctg                                                                                                                 >  1:232073/1‑202 (MQ=255)
                                                                                                                                                      aCTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGACCCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCa                                                                                                                                                                                                      >  2:194060/1‑116 (MQ=255)
                                                                                                                                                      aCTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCg                                                                                                >  2:358415/1‑218 (MQ=255)
                                                                                                                                                          gCCACCAGCACCGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACa                                                                           <  1:228930/235‑1 (MQ=255)
                                                                                                                                                               caGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGTACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGgcc                                                                       <  2:388106/234‑1 (MQ=255)
                                                                                                                                                               caGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGgccg                                                                      <  1:358416/235‑1 (MQ=255)
                                                                                                                                                                   aaCGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACa                                                                           <  2:501547/226‑1 (MQ=255)
                                                                                                                                                                           gggcTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGACCCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCa                                                                                          <  1:91564/200‑1 (MQ=255)
                                                                                                                                                                                         cgcgcTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCTGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCa                                                                                          >  2:698791/1‑189 (MQ=255)
                                                                                                                                                                                         cgcgcTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCTGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCa                                                                                          <  1:698792/189‑1 (MQ=255)
                                                                                                                                                                                         cgcgcTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACa                                                                           <  2:263503/204‑1 (MQ=255)
                                                                                                                                                                                         cgcgcTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAgg                                           >  1:545688/1‑236 (MQ=255)
                                                                                                                                                                                          gcgcTGGATAACTTCGGCATCGCTGCCCCTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGTCCCGCGCTTTCATCTTCGCCACGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTg                                                                                                  >  2:40361/1‑180 (MQ=255)
                                                                                                                                                                                          gcgcTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCTGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTgg                                                                                                                        <  1:425020/158‑1 (MQ=255)
                                                                                                                                                                                          gcgcTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCTGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTgg                                                                                                                        >  2:425019/1‑158 (MQ=255)
                                                                                                                                                                                           cgcgGGATAACTTCGGCAGCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCct                                                                                                                  <  2:208329/163‑1 (MQ=255)
                                                                                                                                                                                                 aTAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAGGCCATCaaa                                   <  1:575572/236‑1 (MQ=255)
                                                                                                                                                                                                     cctCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCg                                                                                                <  2:660927/169‑1 (MQ=255)
                                                                                                                                                                                                      ttCGGCATCGCTGCCACTGACCCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAg                                                                              <  1:253262/188‑1 (MQ=255)
                                                                                                                                                                                                           cATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCAt                                                                  >  2:160357/1‑195 (MQ=255)
                                                                                                                                                                                                            aTCGCTGCCACTGAGCCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCccacca                                             <  1:353182/215‑1 (MQ=255)
                                                                                                                                                                                                                       tGAACCCGAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAGGCCATCAAAATTCACt                            <  2:647042/221‑1 (MQ=255)
                                                                                                                                                                                                                         aaCCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGCGGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGt                                                        <  2:430684/191‑1 (MQ=255)
                                                                                                                                                                                                                            cccAGCCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTGGAAGCCCATCTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCa                                                                                          <  2:167275/154‑1 (MQ=255)
                                                                                                                                                                                                                                 gcggcgCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAGGCCATCAAAATTCACTTCATAGGATTTCATCGGCTAGGCt    <  1:160357/235‑1 (MQ=255)
                                                                                                                                                                                                                                   ggcgCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAGGCCATCAAAATTCACTTCATAGGATTTCATCGGCTAGGCTcc  >  1:681499/1‑235 (MQ=255)
                                                                                                                                                                                                                                     cgcAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCgg                                                                                                                                       <  2:113582/100‑1 (MQ=255)
                                                                                                                                                                                                                                     cgcAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTAAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACa                                                                           <  1:172619/160‑1 (MQ=255)
                                                                                                                                                                                                                                      gcAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGgccg                                                                      <  2:477729/164‑1 (MQ=255)
                                                                                                                                                                                                                                      gcAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGgccg                                                                      >  1:477730/1‑164 (MQ=255)
                                                                                                                                                                                                                                        aGCGCCTCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCGGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTtgt                                                            <  1:17393/172‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     
GCCCAGCACGCGGCTGGTGGTCGGGTGCTCGATGCTGCGGTGATATTTGCAGTTGAGGTTGGCGGCGGTGAAGTGCACGCGACCGTCAGCAGTGTCGGCACTCGGGCTGACTGTTGCGGCGTTGGCCACCCACATGCTCGAGGCCGAGCAACTGGCCACCAGCAACGGCATGGCCTCGCGGGCGGCGCGCTGGATAACTTCGGCATCGCTGCCACTGAACCCCAGGCGGCGCAGCGCCGCCACGTCCGGGCGCTCTTGTGGCGCCAGAACACCCTGCTTGAAGCCCATGTCGGCCAGCGCTTTCATCTTCGCCAGGCCCTGGCGCGCGGCCTCACGCGGGTTGGACGCCTGCTGGCTATTGCTCTGCGAAGCCACGTTACCGTAAGACAGGCCGCCATAGTTGTGGGTAGGCCCCACCAGGCCATCAAAATTCACTTCATAGGATTTCATCGGCTAGGCTCC  >  AE015451/5086873‑5087334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑