| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 197,866 | G→C | 20.3% | A1124A (GCG→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 197,866 | 0 | G | C | 20.3% | 82.8 / 11.3 | 34 | A1124A (GCG→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (21/6); new base C (5/2); total (26/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACCAGCAGCCTTC > AE015451/197632‑198084 | tGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCgg < 1:356433/236‑1 (MQ=255) tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGt > 1:374371/1‑236 (MQ=255) cacaGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACAGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGAtt > 1:408478/1‑236 (MQ=255) aCGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCgg < 2:301052/211‑1 (MQ=255) aCTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTGGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAgc > 1:173483/1‑179 (MQ=255) accacTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCa > 1:489647/1‑229 (MQ=255) acaaccgCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACTGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATc > 2:298228/7‑216 (MQ=255) ctGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTc > 1:391242/1‑236 (MQ=255) tGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGAtt > 1:375379/1‑155 (MQ=255) aCCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCg > 1:255129/1‑183 (MQ=255) aCCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGGAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCgg > 2:129402/1‑157 (MQ=255) cGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCTGCCaa > 1:267810/1‑159 (MQ=255) gCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGACGCggt > 1:139431/1‑118 (MQ=255) gCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGc > 1:31945/1‑189 (MQ=255) gCCAAGCGTGACCGAAGGCTGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCgg < 1:408480/113‑1 (MQ=255) aaaGCGTGACCGAAGTCGGCGTGATCACCTACACCGTGACCCTGAGCATTCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTATAACCCAGGGCAGCGTCTATTTCCAGACCCCCGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCg < 2:267809/191‑1 (MQ=255) aCCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTacaa < 2:375378/155‑1 (MQ=35) tGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCACTGACGTCTACAACAACGGCTCGACCGTCAGCGTCCCCATCGAGAGCGCCACGGGCGGCAACTTCGAACAt > 2:44266/1‑185 (MQ=255) aCCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATAc < 2:391241/236‑1 (MQ=255) cccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCgg < 2:491599/72‑1 (MQ=39) cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCa < 2:270681/139‑1 (MQ=255) cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCa > 1:270682/1‑139 (MQ=255) cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCCGACTCCGAATCCGACCCCAGCCTCGACTg > 1:47356/1‑211 (MQ=255) cTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACTGTCAGCGTCACCATCGAGAACGCCACGGGCGGCAACTTCGAACATCTGACTCCGAACCCGACCCCAg > 2:483864/1‑202 (MQ=11) tGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGACCGCCACGGGCGGCaagt > 2:268237/1‑159 (MQ=11) ccGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCCGCGTCACCATCGAGAgcgc > 2:416577/1‑137 (MQ=38) ccGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGtcatca > 1:222632/1‑196 (MQ=255) gacagTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGa > 2:599031/5‑195 (MQ=255) aaCCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGa > 1:220344/1‑158 (MQ=40) cagTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAgc < 1:599032/225‑1 (MQ=255) ccAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGt < 2:222632/196‑1 (MQ=255) cAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTg > 1:22051/1‑209 (MQ=255) ccATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCCATGGCAATGTGACCGAAGACCAGCAGccttc > 1:152029/1‑235 (MQ=255) ccATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGa > 2:536284/1‑156 (MQ=255) aaGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGt > 2:38892/1‑172 (MQ=255) aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGAc < 2:643916/133‑1 (MQ=255) aaGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACAGGCGGCAACTTCGAACATCTGACTCCGAATCCGAc > 1:643917/1‑133 (MQ=255) gCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCCAGAGCGCCACGggc > 1:88074/1‑99 (MQ=18) | TGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCACCGACTCGGTCGACACCACTACTGCGACCCTGACCGCCTCGCCAAGCGTGACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAACCATTACCGTTGAAGCGGGTAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGCTCGACCGTCAGCGTCACCATCGAGAGCGCCACGGGCGGCAACTTCGAACATCTGACTCCGAATCCGACCCCAGCCTCGACTGTCATCAATGACAGCATCGATACCGTCACCGTAAGTATTGTCAGCAATGGCAATGTGACCGAAGACCAGCAGCCTTC > AE015451/197632‑198084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |