Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 302,935 G→C 22.2% G367A (GGT→GCT)  envZ → Osmolarity sensor protein EnvZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451302,9350GC22.2% 62.5 / 14.9 27G367A (GGT→GCT) envZOsmolarity sensor protein EnvZ
Reads supporting (aligned to +/- strand):  ref base G (12/9);  new base C (4/2);  total (16/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GTGAGGTGGTGGCTCCGTACAACCAGCCGGAAGAGCGTGTGCGCCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCT  >  AE015451/302713‑303164
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     
gTGAGGTGGTGGCTCCGTACAACCAGCCGGAAGAGCGTGTGCGCCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGCTATTGACGAGTCg                                                                                                                                                                                                                          >  1:144202/1‑236 (MQ=255)
            cTCCGTACAACCAGCCGGAAGAGCGTGTGCGCCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGaaa                                                                                                                                                                                                                <  2:627296/234‑1 (MQ=255)
                  aCAACCAGCCGGAAGAGCGTGTGCGCCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCAt                                                                                                                                                                                                            >  1:117561/1‑232 (MQ=255)
                              gagagCGTGTGCGCCTGTGCCTGGCGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGCTATTGACGAGTCGGAGCTGGa                                                                                                                                                                                                                  <  1:176363/213‑1 (MQ=255)
                                       tgCGCCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTg                                                                                                                                                                                     >  1:165975/1‑234 (MQ=255)
                                         cgcCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAAc                                                                                                                                                                                                     <  1:749770/216‑1 (MQ=255)
                                           ccTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACg                                                                                                                                                                                                                               >  1:400525/1‑188 (MQ=255)
                                              gtgCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCa                                                                                                                                                                             <  1:509506/235‑1 (MQ=255)
                                                                                                     cTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAggg                                                                                                                                                               <  2:522048/194‑1 (MQ=255)
                                                                                                            aCCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGTGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGATGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGAt                                                                                                                                                                                   >  2:569663/1‑167 (MQ=255)
                                                                                                                             cTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAgg                                                                                                                                                                <  2:437449/169‑1 (MQ=255)
                                                                                                                                 aCCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGCTATTGCCGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGgtcgtc                                                                                                                                      >  2:86068/1‑191 (MQ=255)
                                                                                                                                       cTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGCTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCt                                                                                   >  1:516655/1‑236 (MQ=255)
                                                                                                                                        tGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTg                                                                                  >  1:620154/1‑236 (MQ=255)
                                                                                                                                        tGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTg                                                                                  >  1:607999/1‑236 (MQ=255)
                                                                                                                                                                        cAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCg                                                                                                                                 >  2:167667/1‑157 (MQ=255)
                                                                                                                                                                          ggTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGCTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGTATTGCTGCGCAACATGGCGGGGACGTgggggtgg                                                                                              >  2:169971/1‑184 (MQ=255)
                                                                                                                                                                            tGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACgtgca                                                                                                                                                                       >  1:44062/1‑111 (MQ=255)
                                                                                                                                                                                 agagTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCggg                                                                                                                                                                               <  1:643576/102‑1 (MQ=255)
                                                                                                                                                                                 agagTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCggg                                                                                                                                                                               >  2:643575/1‑102 (MQ=255)
                                                                                                                                                                                              tgtgGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctg                                   <  2:607998‑M1/229‑11 (MQ=255)
                                                                                                                                                                                                   tgCTCAGTGTGCTGGACCGGGGCACCGCTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGTATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCt                       <  1:169971/236‑1 (MQ=255)
                                                                                                                                                                                                       cAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACCTGGCGGGaa                                                                                                         >  2:750718/1‑150 (MQ=255)
                                                                                                                                                                                                                  gcccgggCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATtgct                                                                                                                          <  2:248017/118‑1 (MQ=255)
                                                                                                                                                                                                                         cACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCctct  >  1:449193/1‑235 (MQ=255)
                                                                                                                                                                                                                         cACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGGCTGGCGGCGGGGTCGGGGCGCgg                                                              >  1:199686/1‑175 (MQ=255)
                                                                                                                                                                                                                            cGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGgctgctgctgctgccg                             <  2:396133‑M1/205‑17 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                     
GTGAGGTGGTGGCTCCGTACAACCAGCCGGAAGAGCGTGTGCGCCTGTGCCTGGAGCCGATTCCACCGTTCCCGCTGCGCCGGGTTTCGCTCAAGCGCATGCTGGGCAACCTGATCGGCAACGCCCTGCACCATGCTGGCAAGGGGGTCGAGGTGGCTGCCTATGTGTCAGGTGACGAGAGTGCGCCGTATGTGGTGCTCAGTGTGCTGGACCGGGGCACCGGTATTGACGAGTCGGAGCTGGAAACCATCTTCAACCCGTTCATTCGCGGTGATCGGGCACGGGGCGGCAAGGGCACCGGGCTGGGGCTGGCGATCGTCAAGCGGATTGCTGCGCAACATGGCGGGAACGTGGAGTTGCGTAACCGGTCTGGCGGCGGGATCGAGGCGCGGGTCAGGTTGCCGCTGGGGCTGCTGCTGCCGCGTAATGCTGTGTGAGGCTCACTGGCCTCT  >  AE015451/302713‑303164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: