| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 924,321 | G→A | 10.4% | A291V (GCG→GTG) ‡ | PP_0804 ← | Protein secretion ABC efflux system, permease and ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 924,321 | 0 | G | A | 10.4% | 110.3 / 4.0 | 39 | A291V (GCG→GTG) ‡ | PP_0804 | Protein secretion ABC efflux system, permease and ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (15/19); new base A (2/2); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
GATACTTTCCACCAGCATGGCGTTGCGCAGCGCCGACTCACGCATCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAACACCAGTGCCAACACCACACCGCCAAAC > AE015451/924119‑924545 | gATACTTTCCACCAGCATGGCGTTGCGCAGCGCCGACTCACGCATCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACt < 1:327861/236‑1 (MQ=255) aCTTTCCACCAGCATGGCGTTGCGCAGCGCCGACTCACGCATCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACt < 2:324354/233‑1 (MQ=255) aGCATGGCGATGCGCAGCGCGGACTCACGCATCGAGGCATTGGCCAACCGTGCCAGGCGCCGTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACCAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCgcgc > 2:397452/1‑234 (MQ=255) tGCGCAGCGCCGACTCACGCATCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgag > 2:705821/1‑233 (MQ=255) gCATCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACtt < 1:317855/235‑1 (MQ=255) tCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTc < 1:377483/199‑1 (MQ=255) ccGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTg < 2:615955/232‑1 (MQ=255) gTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACGGTCGCGATGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCa > 1:730118/1‑222 (MQ=255) gTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcag > 1:664024/1‑235 (MQ=255) aGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTcgcg < 2:550682/161‑1 (MQ=255) ggCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTg < 1:388524/224‑1 (MQ=255) gcgcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGcgcaggcgcagg < 1:374772/235‑1 (MQ=255) gcgcCTTGGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAggg < 1:752117/236‑1 (MQ=255) aCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg < 1:143368/185‑1 (MQ=255) ggCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCGGGACGTGATCAGGGCGCGGAGCGACTCCCGCTCgcgc > 2:114553/1‑156 (MQ=255) ggCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGGCGGCCAGCACCGCGACGGGGCTGGACGGGAACAGGTc > 2:143368/1‑134 (MQ=255) agcaCCATGGCCAGCAGCGTGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCTCGGTGCTGGAAGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg > 1:399685/1‑177 (MQ=255) cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCt < 1:541012/111‑1 (MQ=255) cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCt > 2:541011/1‑111 (MQ=255) ccAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGGGa > 1:146340/1‑114 (MQ=255) gACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGCCGGCCAGCACCGTCGCGGTGCTGGACgg > 2:103553/1‑101 (MQ=255) cAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCGGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGa < 1:669057/236‑1 (MQ=255) cAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgcgc < 1:13027/229‑1 (MQ=255) aGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGGGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCAGGGCCGTAGACCAGGTCGCTCACCCGCa > 1:223480/1‑217 (MQ=255) gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGGGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAggt < 1:114553/236‑1 (MQ=255) gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAggt < 1:218318/236‑1 (MQ=255) aTGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATg < 2:567089/185‑1 (MQ=255) ccAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAggtcgg > 2:284455/1‑231 (MQ=255) cgccgcCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACt > 2:331051/1‑138 (MQ=255) cgccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCg > 1:718607/1‑235 (MQ=255) cagATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGa < 1:363561/173‑1 (MQ=255) aGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCt > 2:560982/1‑179 (MQ=255) gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGACGCAGCAGGCGCATGCTGAAGTCGAac < 1:397453/235‑1 (MQ=255) acagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAacac > 1:664082/1‑235 (MQ=255) aCGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAACACCAGTGCCa > 2:659253/1‑231 (MQ=255) cacgtccAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCGTTAGCCGCAGCCGgc > 1:199265/6‑197 (MQ=255) tCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAACACCAGTGCCAACACCACACCGcc < 1:659254/236‑1 (MQ=255) cGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAACACCAGTGCCAACACCACACCGCCa < 1:352266/236‑1 (MQ=255) gCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAACACCAGTGCCAACACCACACCGCCAAAc < 2:718606/233‑1 (MQ=255) | GATACTTTCCACCAGCATGGCGTTGCGCAGCGCCGACTCACGCATCGAGGCATTGGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCCGAGCAGGTCGGTCACCTTTAGCCGCAGCAGGCGCATGCTGAAGTCGAACACCAGTGCCAACACCACACCGCCAAAC > AE015451/924119‑924545 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |