| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 2,128,535 | G→T | 8.3% | intergenic (+33/+17) | PP_1887 → / ← PP_1888 | conserved protein of unknown function/Fimbrial protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 2,128,535 | 0 | G | T | 8.3% | 162.3 / 7.0 | 48 | intergenic (+33/+17) | PP_1887/PP_1888 | conserved protein of unknown function/Fimbrial protein |
| Reads supporting (aligned to +/- strand): ref base G (22/22); new base T (2/2); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-02 | |||||||||||
AATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCATCTGAATACCTATGCCACGGGCTGCGGAGTCGTCGGTCA > AE015451/2128315‑2128763 | aaTACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCtc > 1:13130/1‑230 (MQ=255) gCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTa < 1:457395/236‑1 (MQ=255) tGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCa < 1:328417/236‑1 (MQ=255) tACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCa < 1:610835/236‑1 (MQ=255) atgaGCAGGGGCGGGTACTGACGGATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCa < 2:13130/203‑1 (MQ=255) aGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGTACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCtcgtcg > 1:351476/1‑168 (MQ=255) ggTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTCCCTATACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCtt < 1:412020/200‑1 (MQ=255) ggTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTCCCTATACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCtt > 2:412019/1‑200 (MQ=255) aCTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTCCCTATACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTgc > 2:457614/1‑211 (MQ=255) aCTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTCCCTATACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTgc < 1:457615/211‑1 (MQ=255) aCTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGt > 2:742474/1‑209 (MQ=255) aCGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCa < 2:352229/188‑1 (MQ=255) gACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCtcgtc > 1:705032/1‑147 (MQ=255) ccGTTTGCGCAGGGTCGAGACCCCTGTCGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGcc < 2:210754/180‑1 (MQ=255) aCCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTgg < 2:46515/186‑1 (MQ=255) ccccTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTc > 1:290875/1‑235 (MQ=255) cGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCa < 1:645843/144‑1 (MQ=255) gCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCa < 1:280597/131‑1 (MQ=255) gCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCa > 2:280596/1‑131 (MQ=255) aCAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAgg < 2:466347/151‑1 (MQ=255) aCTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTg < 1:742475/235‑1 (MQ=255) aTGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCa > 2:383686/1‑93 (MQ=255) cccAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTc > 1:207125/1‑201 (MQ=255) cAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCGTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGcc > 2:455520/1‑129 (MQ=255) cAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCGGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCa > 1:220935/1‑236 (MQ=255) agagAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTa < 2:310523/192‑1 (MQ=255) gaACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGt < 2:145654/128‑1 (MQ=255) ccGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGTCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTgg < 2:321857/134‑1 (MQ=255) cGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGtt > 2:252786/1‑126 (MQ=255) cGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGtt < 1:252787/126‑1 (MQ=255) cTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTg < 1:641198/223‑1 (MQ=255) cTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTg > 2:641197/1‑223 (MQ=255) cTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCATCTGaa > 1:654195/1‑236 (MQ=255) gtaaaGGGAACGGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAg < 1:273413/222‑1 (MQ=255) gCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCATCTGAATACCTTTGc > 1:599901/1‑235 (MQ=255) aCCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGt > 2:314781/1‑162 (MQ=255) aCCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGt < 1:314782/162‑1 (MQ=255) ggggggCGACGAGGCCGGTATAGGGACCGGCCtcgt > 2:486937/1‑36 (MQ=255) gggggCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGt > 1:45543/1‑147 (MQ=255) gggggCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGt < 2:45543/147‑1 (MQ=255) cgacgaGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCATCTGAATACCTATGCCACGGGCt > 1:747625/1‑231 (MQ=255) gacgaGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGAtc < 1:513805/170‑1 (MQ=255) gacgaGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGAtc > 2:513804/1‑170 (MQ=255) acgaGGCCGGTATAGGGACCGGCCtcg < 2:210556/27‑1 (MQ=255) aGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGc > 2:633911/1‑181 (MQ=255) ggataGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCAt < 2:536038/196‑1 (MQ=255) gTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCATCTGAATACCTATGCCACGGGCTGCGGAGTCGTCGGTCa > 1:477435/1‑236 (MQ=255) aGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGTCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGGTGTACAGCATCTGAATACCTATGCCACGGGc > 1:235385/1‑215 (MQ=255) | AATACGATCCGCAGTTGGCTATCGATTTGCATTACGACGGCAATGGCAACCTGATCAGTGATGAGCAGGGGCGGGTACTGACGTATGACGGGCTCAACCGTTTGCTCAGGGTCGAGACCCCTGACGGTGAACGCTGTCGCTACAACTATGACCCAGAGAACATTCTGTCCGGCACGGCCCCTGGCTAAGGGCACCGGGGGGGCGACGAGGCCGGTATAGGGACCGGCCTCGTCGCATTCAGCGGTAGCGAATGGTGAAGTTCAAGGCACCCTTGGCCAAGCCCGTGCGCAGGCTGCTGTTGGGTTCTGTCTGGAAGAAGCGGGCCTGAAAATTCAAAGGCACGTTATCGCTCGTCAGGGGTACCAGGTCGATCTCTTTGTTCAGGGGCATCGATACCCCGTTGTACAGCATCTGAATACCTATGCCACGGGCTGCGGAGTCGTCGGTCA > AE015451/2128315‑2128763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |