Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	2,550,125	G→A	50.0%	noncoding (1436/1522 nt)	PP_16SF →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	2,550,125	0	G	A	50.0%	16.8 / 64.0	40	noncoding (1436/1522 nt)	PP_16SF	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (11/9);  new base A (9/11);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01

CCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGCAGTTCAAATCTG  >  AE015451/2550052‑2550360                                                                          |                                                                                                                                                                                                                                            ccGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGa                                                                           <  1:684339/236‑1 (MQ=11)          gTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTta                                                                   <  2:93349/235‑1 (MQ=31)          gTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTtat                                                                  >  1:451724/1‑236 (MQ=14)            acacacCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTtatat                                                                >  2:529935/1‑235 (MQ=14)                 ccGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTg                                                           <  1:315976/236‑1 (MQ=14)                 ccGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTg                                                           <  1:354402/236‑1 (MQ=14)                  cGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAAcgcg                                                                            >  1:569863/1‑218 (MQ=11)                   gCCCGTCCCACCATGGGAGTGGGTTGCATCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAAcgcg                                                                            <  2:266268/217‑1 (MQ=11)                   gCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCt                                                            >  1:36763/1‑233 (MQ=31)                   gCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTa                                                         <  1:562735/236‑1 (MQ=14)                       gTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTc                                                     >  1:463219/1‑236 (MQ=31)                       gTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTc                                                             >  2:359488/1‑228 (MQ=14)                          acacCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAAcgcg                                                                            <  2:566298/210‑1 (MQ=11)                           cacCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGc                                                       <  2:730529/230‑1 (MQ=31)                                  ggAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTaga                                          <  1:631078/236‑1 (MQ=14)                                    aGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAgc                                        <  1:467642/236‑1 (MQ=31)                                    aGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAgc                                        <  1:93911/236‑1 (MQ=31)                                     gTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAgc                                        <  1:374845/235‑1 (MQ=31)                                         gTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGc                                                       <  1:225529/216‑1 (MQ=31)                                         gTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGc                                                       >  2:225529/1‑216 (MQ=31)                                         gTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCAcc                                   >  1:467351/1‑236 (MQ=31)                                         gTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCAcc                                   >  1:690447/1‑236 (MQ=14)                                          ttGCACCAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAgcgc                                      <  1:281053/232‑1 (MQ=14)                                           tGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTtata                                                                 >  1:636341/1‑204 (MQ=31)                                           tGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTtata                                                                 <  2:636340/204‑1 (MQ=31)                                               ccAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCAc                                    <  2:459434/229‑1 (MQ=14)                                                cAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATa                            >  1:747731/1‑236 (MQ=31)                                                cAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATa                            >  1:98869/1‑236 (MQ=31)                                                cAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTACCGCGACCCTGTTATAGGTCTGTAGCTCAg                                                   >  1:29093/1‑213 (MQ=14)                                                cAGAAGTAGCTAGTCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATTGGTCTGTAGCTCAGTTgtt                                              >  1:55922/1‑216 (MQ=14)                                                     gTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTa                                                         <  1:669407/202‑1 (MQ=31)                                                             tCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTAATAGGTCTGTa                                                         >  2:560382/1‑194 (MQ=14)                                                             tCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAg                                                                <  2:305964/187‑1 (MQ=14)                                                             tCTAACCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAg                                                                >  1:305965/1‑187 (MQ=17)                                                                aaCCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGCCGATGCTGTACCGCGACCCTGTTATAGGTCTGTAGCTCCGTTGGTTagag                                         >  2:209244/1‑207 (MQ=14)                                                                aaCCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCCCCCCTGATa                            >  1:247789/1‑220 (MQ=14)                                                                aaCCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATaa                           >  1:53966/1‑221 (MQ=31)                                                                aaCCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATAAGGGTGAGGt                  >  1:502936/1‑230 (MQ=14)                                                                 aCCTTCGGGAGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGCa             <  1:59761/234‑1 (MQ=14)                                                                  ccTTCGGGAGGACGGTTACCACGGTGTGATTCGTGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAgcgc                                      <  2:208339/208‑1 (MQ=14)                                                                       gggaggACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGCAGTTCa        <  2:385889/233‑1 (MQ=14)                                                                         ggggACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAATGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATAAGGGTGa                     >  2:293723/1‑218 (MQ=14)                                                                          gggACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACGCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGGTAAGGGGGAGGTCGGCAGTGCAAGTCTg  >  1:140836/1‑236 (MQ=9)                                                                          |                                                                                                                                                                                                                                            CCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCACCAGAAGTAGCTAGTCTAACCTTCGGGGGGACGGTTACCACGGTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTAAAAGACGATGCTGTAACGCGACCCTGTTATAGGTCTGTAGCTCAGTTGGTTAGAGCGCACCCCTGATAAGGGTGAGGTCGGCAGTTCAAATCTG  >  AE015451/2550052‑2550360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑