Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	3,142,272	C→T	100%	A74V (GCG→GTG)	PP_2758 →	putative Ribose ABC transporter, periplasmic ribose‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	3,142,272	0	C	T	100.0%	120.7 / NA	33	A74V (GCG→GTG)	PP_2758	putative Ribose ABC transporter, periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/17);  total (16/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGAGGTGACCAATGAGTGACAACGACTGCTCCGGCATGTCCCGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGCGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATCCCGCTGTTCACCATCGAGGTGCCGTCGCAGT  >  AE015451/3142041‑3142487                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        cGAGGTGACCAATGAGTGACAACGACTGCTCCGGCATGTCCCGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCa                                                                                                                                                                                                                      <  1:614729/235‑1 (MQ=255)                          cTGCTCCGGCATGTCCCGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCg                                                                                                                                                                                                                 >  1:633029/1‑215 (MQ=255)                                 ggCATGTCCCGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGcc                                                                                                                                                                                     <  1:642198/236‑1 (MQ=255)                                     tGTCCCGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTgg                                                                                                                                                                                               <  1:130392/222‑1 (MQ=255)                                        cccGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGa                                                                                                                                                                                                                <  1:336732/202‑1 (MQ=255)                                                          gcgcGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCaa                                                                                                                                                             >  1:620245/1‑235 (MQ=255)                                                               tCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTa                                                                                                                                                                                                         >  1:283266/1‑186 (MQ=255)                                                                                                         cgcgCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCa                                                                                                                                                                                                                      >  2:675582/1‑131 (MQ=255)                                                                                                         cgcgCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCa                                                                                                                                                                                                                      <  1:675583/131‑1 (MQ=255)                                                                                                            gcCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCg                                                                                                           <  1:632287/235‑1 (MQ=255)                                                                                                              cATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATg                                                                                                        <  1:652502/236‑1 (MQ=255)                                                                                                                    ggcTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGCATCTGGTGa                                                                                                                       >  2:604062/1‑215 (MQ=255)                                                                                                                           gCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCacac                                                                                           >  1:585974/1‑236 (MQ=255)                                                                                                                                      tGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGggcg                                                                                                                                                                                            >  2:377173/1‑128 (MQ=255)                                                                                                                                                   gCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATTGTGACCCAGTTGCa                                                                                                                                  >  1:49218/1‑173 (MQ=255)                                                                                                                                                   gCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGCAGCCCGATGCAGTTATTca                                                                                              >  1:664330/1‑209 (MQ=255)                                                                                                                                                     cacaGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATgg                                                                                                                                               >  2:380853/1‑158 (MQ=255)                                                                                                                                                           aCCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTGCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTg                                                                                                                                             <  2:177388/154‑1 (MQ=255)                                                                                                                                                               tCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCaa                                                                                                                                                             <  2:334120/134‑1 (MQ=255)                                                                                                                                                               tCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCaa                                                                                                                                                             >  1:334121/1‑134 (MQ=255)                                                                                                                                                                 gCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGcc                                                                                                                                                                                     <  2:480725/108‑1 (MQ=255)                                                                                                                                                                             gcgcATCGACATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCAGGTGACGCTGAAGCCCGATGCAGtt                                                                                                   >  1:415581/1‑178 (MQ=255)                                                                                                                                                                              cgcATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCa                                                                                                                                                                                                                      <  2:283265/62‑1 (MQ=255)                                                                                                                                                                                     ccATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCa                                                  <  2:620244/219‑1 (MQ=255)                                                                                                                                                                                          aGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGcgccg                                         <  1:211571/223‑1 (MQ=255)                                                                                                                                                                                                gTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAAcca                                                                                                                                                          >  2:6554/1‑102 (MQ=255)                                                                                                                                                                                                     cACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCa                                                  >  2:377424/1‑203 (MQ=255)                                                                                                                                                                                                              cTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATc                                 <  2:336792/211‑1 (MQ=255)                                                                                                                                                                                                              cTACTTCGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATc                                 >  1:336793/1‑211 (MQ=255)                                                                                                                                                                                                                    cGACAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATCCCGCTGTTCACCATCGAGGTGCCGt        <  1:43875/230‑1 (MQ=255)                                                                                                                                                                                                                      aCAGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATCCCGCTGTTCACCATCGAGGTGCCGTCGCAGt  <  2:585973/234‑1 (MQ=255)                                                                                                                                                                                                                        aGCCGGGCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATCCCGCTgg                          >  2:183034/1‑207 (MQ=255)                                                                                                                                                                                                                             ggCCTTCAAGGTGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATCCCg                              <  1:135340/199‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                        CGAGGTGACCAATGAGTGACAACGACTGCTCCGGCATGTCCCGGCGCTGCCTGCTCGGCGCGTCCTCGGCGATGCTCGCTCTCGGGCTTGCCGCACCATTGACCCGCGCCATGGCGGCTGGAGCCGACAGCGGTGCCGAGGCCAGTGCCACAGTACCTTCGCTGAAGGGCAAGCGCATCGCCATCAGCACCGTGGGCACCAGCATCTACTTCGACAGCCGGGCCTTCAAGGCGCAGGTCGAGGAAGTACAGCGCCTGGGCGGCACGCCGATCACCTTGGATGCCGGGCGCAACGACAAGGCCATGGTGACCCAGTTGCAGAATCTGGTGACGCTGAAGCCCGATGCAGTTATTCACACCCTCGGCACGCTGAGCATCATCGAGCCCTGGTTCAAGCGCATCAGCGCCGCCGGCATCCCGCTGTTCACCATCGAGGTGCCGTCGCAGT  >  AE015451/3142041‑3142487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑