| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 1,499,506:1 | +C | 100% | intergenic (+83/‑81) | trpS → / → zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 1,499,506 | 1 | . | C | 94.6% | 110.9 / 1.0 | 37 | intergenic (+83/‑81) | trpS/zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (6/28); total (7/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCAC‑G‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCAC > AE015451/1499275‑1499700 | gCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAc‑g‑ccc < 1:976396/236‑2 (MQ=255) cGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAC‑GCCC‑GCTCCCACAGAc < 2:963624/234‑1 (MQ=255) gCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCAtt < 1:505143/235‑1 (MQ=255) gCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAc‑g‑c < 1:185207/183‑1 (MQ=255) gAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCGATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCGAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCgg < 1:628820/212‑1 (MQ=38) gTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAc‑g‑c < 2:1023403/175‑1 (MQ=255) gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGt < 1:263457/215‑1 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa > 1:685743/1‑236 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCGGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 2:442639/206‑1 (MQ=37) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 1:549513/236‑1 (MQ=39) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:64500/229‑1 (MQ=255) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAACCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:182658/236‑1 (MQ=38) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:702210/223‑1 (MQ=255) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATc > 1:1074459/1‑214 (MQ=38) ggCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACCGCAGGACTGGGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:898299/215‑1 (MQ=37) ccATTCGGAGCCTGAACGACGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCggg < 2:412883/161‑1 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GACC‑GCTCCCACAGACTGATTGCCATTAAGCgg < 2:687781/158‑1 (MQ=25) tGAACGAAGCCTGGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGGTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTc < 1:136674/186‑1 (MQ=37) aCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 1:973616/236‑1 (MQ=255) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:1109014/215‑1 (MQ=38) ccacAGCAGGACTGAGGCGGCCAAACGGTGGGGGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:97662/182‑1 (MQ=37) cagcagGACTGAGGCTGCCGAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:692351/179‑1 (MQ=25) gcagGACGGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAc < 1:255544/232‑1 (MQ=38) gACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGgcgc < 1:711367/235‑1 (MQ=255) cTGGCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGGCCGTGTTCG‑CGGGCACGG‑CC‑GCCCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:1009008/175‑1 (MQ=37) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑G‑CCTGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATCACTCCCTTAgcc > 2:97913/1‑176 (MQ=25) tGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:896619/174‑1 (MQ=25) tGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTGTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 1:607356/184‑1 (MQ=37) ggggCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:659287/149‑1 (MQ=25) gCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTT‑CGCGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGc > 1:576409/1‑222 (MQ=39) gTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAgcgc > 2:1082936/1‑234 (MQ=255) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGGGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCAt > 1:924186/1‑193 (MQ=255) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:143629/130‑1 (MQ=37) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGAGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATc < 2:1130619/151‑1 (MQ=37) cgcgGTGCATGGCACCGGCTTTGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCAc < 1:916416/236‑1 (MQ=255) tGCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGCCTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAgc < 1:642733/192‑1 (MQ=255) gCCGGTGTTCG‑CGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt < 2:657196/132‑1 (MQ=39) gttttcgcGGGCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCATATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGActt > 2:846228/8‑143 (MQ=255) gggCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGc < 1:1043081/162‑1 (MQ=255) ggCAC‑GCCC‑GCTCCCACAGACTGATTGCCATTGAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGGCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTAcgacga < 1:770177/191‑1 (MQ=255) | GCGAGGCCGATGTCCGCATCGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGTGTT‑T‑CGGGCAC‑G‑CC‑GCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCAC > AE015451/1499275‑1499700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |