Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	1,932,638:1	+C	100%	intergenic (‑105/+45)	rluA ← / ← minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,932,638	1	.	C	100.0%	150.6 / NA	41	intergenic (‑105/+45)	rluA/minE	23S rRNA (746) and tRNA (32) pseudouridine synthase/Cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/18);  total (23/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCC  >  AE015451/1932420‑1932857                                                                                                                                                                                                                            |                                                                                                                                                                                                                            cAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCa                                                                                                                                                                                                              <  1:572031/235‑1 (MQ=255)     cAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGc                                                                                                                                                                                                          >  1:691661/1‑235 (MQ=255)            tgttgtCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCaa                                                                                                                                                                                                  <  1:191053/236‑1 (MQ=255)                      ggCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAc                                                                                                                                                                                         >  1:869381/1‑235 (MQ=255)                         cgggCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGc                                                                                                                                                                                                               <  2:310952/208‑1 (MQ=255)                            gCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGc                                                                                                                                                                                                       <  1:224930/215‑1 (MQ=255)                                          gcagcagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccgcg                                                                                                                                                                                                                       >  1:150181/1‑180 (MQ=255)                                              cagGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccgcg                                                                                                                                                                                                                       >  2:1122176/1‑176 (MQ=255)                                                            tgATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCg                                                                                                                                                                           >  2:958215/1‑211 (MQ=255)                                                                                   tCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCaattgt                                                                                                                                                                                              >  1:130638/1‑165 (MQ=255)                                                                                            aTCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGcccc                                                                                                                                                                                                    <  2:1018586/154‑1 (MQ=255)                                                                                                             cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg                                                                                                  <  2:691660/235‑1 (MQ=255)                                                                                                              ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAATGATATGCGTCCCCTGTAGGAGCGGGCTTG‑ccc                                                                                                                                                                                                                          <  2:236573/113‑2 (MQ=255)                                                                                                              ggCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatcat                                                                                                    <  2:869380/232‑1 (MQ=255)                                                                                                                 aTTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cc                                                                                                                                                                                                                           >  2:568546/1‑109 (MQ=255)                                                                                                                     cAGGTCTCACGAAAATAGCAGTCAACGCTGGGTCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGAGATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGCCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                           <  2:51801/170‑1 (MQ=38)                                                                                                                        gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAGGGGACGCATATCCTTTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGCGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTggg                                                                                                                            >  2:227083/1‑197 (MQ=25)                                                                                                                            cACGAAAATAGCAGTAACCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAACGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                                                                                                       <  2:905393/199‑1 (MQ=255)                                                                                                                             aagAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt                                                                                       <  1:255760/228‑1 (MQ=255)                                                                                                                             aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc                                                                                                           >  2:901785/1‑210 (MQ=255)                                                                                                                              cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                           <  1:930150/161‑1 (MQ=255)                                                                                                                                aaaaTAGCAGTAAACGCTGGGGCCGCTTTGCTGCCCTTCGCGTGCAAGCCCGACCCTACAAAGGATATGCGTCCCCTGTAGGTGCAGGCTTG‑ccc                                                                                                                                                                                                                          >  1:812534/1‑94 (MQ=255)                                                                                                                                   aTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCg                                                                           <  1:948664/236‑1 (MQ=255)                                                                                                                                                 tGGGGCCGCTTTGCGGCCCTTAGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg                                                                                                                                                                      <  2:617527/131‑1 (MQ=255)                                                                                                                                                     gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACCAAGGATCTGCGTCCCCTGTCGGAGCGGGCTTGCCCGCCAAggg                                                                                                                                                                                                                   >  2:9994/1‑82 (MQ=21)                                                                                                                                                     gCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACt                                                                    >  1:918492/1‑225 (MQ=255)                                                                                                                                                      ccGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGCTGtt                                                                                       >  2:1027950/1‑205 (MQ=255)                                                                                                                                                          gttGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc                                                                                                                       <  2:1116438/168‑1 (MQ=255)                                                                                                                                                            tGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGc                                                  <  1:901786/236‑1 (MQ=255)                                                                                                                                                                  ccTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTGGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                           <  2:329002/125‑1 (MQ=255)                                                                                                                                                                       gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg                                                                                                            >  2:31921/1‑167 (MQ=255)                                                                                                                                                                         gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCAc                                                                     >  1:598885/1‑204 (MQ=255)                                                                                                                                                                         gggCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGGTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt                                                                                       >  1:148516/1‑186 (MQ=255)                                                                                                                                                                                   gCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGt                             >  1:89630/1‑234 (MQ=255)                                                                                                                                                                                             aGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGGTCAGCGATCCGGCAGGgaga                                                                                                                                                             >  2:348053/1‑96 (MQ=37)                                                                                                                                                                                                atatTCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg                                                                                                                                                           <  2:921095/95‑1 (MQ=255)                                                                                                                                                                                                  atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGCAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCCTCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGCTCTTTCTGCAGcgcg                                            >  2:297463/1‑204 (MQ=255)                                                                                                                                                                                                  atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGttctttct                                                    >  2:1062592/1‑196 (MQ=255)                                                                                                                                                                                                    gCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCCGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCCGCAGTTCTTTCTCCAGCGCggg                                          >  1:305075/1‑204 (MQ=255)                                                                                                                                                                                                    gCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCcgcg            >  2:898284/1‑234 (MQ=255)                                                                                                                                                                                                     cGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCt          >  1:1084550/1‑235 (MQ=255)                                                                                                                                                                                                            tGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTgcgc   >  1:639592/1‑235 (MQ=255)                                                                                                                                                                                                            tGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTgcgc   >  1:978234/1‑235 (MQ=255)                                                                                                                                                                                                               aGGAGCGGGCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGcc  <  2:978233/233‑1 (MQ=255)                                                                                                                                                                                                                      ggCTTGCCCGCGAAGGGCTGCAATGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCGGGGAGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGCTTTCCAGCTCGTTATGCACATCATCGTTGCCGCTGTTca                                                                                     >  2:333301/1‑143 (MQ=255)                                                                                                                                                                                                                       gCTTGCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTGGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGc                >  2:763646/1‑211 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                            CAGGCAGTCCTTGTTGTCCTCGGCACGGCCAGGTACCGACAGCAGCAGGGTCGGTTTGTTGATCACCAGGATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTTGCGGATCACTTCCAGCAGTTCTTTCTGCAGCGCGGGCAGGTAGTCCGGTTCGCTGCGTTGGCCGCGCTCGTGCGCC  >  AE015451/1932420‑1932857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑