Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	2,087,719:1	+A	15.8%	intergenic (+532/+386)	PP_1865 → / ← aroH	transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	2,087,719	1	.	A	15.8%	93.5 / 24.2	38	intergenic (+532/+386)	PP_1865/aroH	transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
Reads supporting (aligned to +/- strand):  ref base . (20/12);  new base A (3/3);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.73e-01

GCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAACCCAAAAGCCCAAAAGCCCAAA  >  AE015451/2087504‑2087945                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    gCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGcc                                                                                                                                                                                                                   <  1:405958/235‑1 (MQ=21) gCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCt                                                                                                                                                                                                                  <  1:805891/236‑1 (MQ=21)                                     ccGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgggg                                                                                                                                                                                              >  1:217197/1‑217 (MQ=255)                                                     cGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTg                                                                                                                                                                                                                                      >  1:637456/1‑164 (MQ=18)                                                      ggCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTGCTCCCGGGAGCAAGGCCGGAGCGAGGGGAcc                                                                                                                                                                        <  2:716762/224‑1 (MQ=11)                                                      ggCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTGCTCCCGGGAGCAAGGCCGGAGCGAGGGGAcc                                                                                                                                                                        >  1:716763/1‑224 (MQ=11)                                                          tGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAgg                                                                                                                                                       <  1:231182/236‑1 (MQ=255)                                                               tACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCCAGCGCTG‑GGCCCCA‑gg                                                                                                                                                                                                                           >  1:647244/1‑163 (MQ=255)                                                                     gaagaaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGGATGGGGCCTGCAGCGCggg                                                                                                                                                                                                        >  2:125003/1‑177 (MQ=11)                                                                          aCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGa                                                                                                                                        >  1:437419/1‑235 (MQ=255)                                                                           ctccGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTACGCGCGCGGTATTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg‑gg                                                                                                                                                                                                                                   <  1:56926/142‑1 (MQ=255)                                                                              cGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCg                                                                                                                                    >  1:1032702/1‑235 (MQ=40)                                                                               gTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGAc                                                                                                                                  >  1:384922/1‑236 (MQ=39)                                                                                                 cttgctTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg                                                                                                                                                                                                <  1:664257/156‑1 (MQ=31)                                                                                                       ttGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGGCGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCg                                                                                                                                                                     <  1:496446/177‑1 (MQ=14)                                                                                                       ttGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGGCAAGCGCTG‑GGCCCCA‑GGAAGGGGCCt                                                                                                                                                                                                                  >  2:919088/1‑132 (MQ=17)                                                                                                       ttGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGtttt                                                                                                                            <  1:898839/218‑1 (MQ=21)                                                                                                                 cTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCTTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGcgtagcgtag                                                                                                                                                        >  1:1074319/1‑180 (MQ=17)                                                                                                                     tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACttttt                                                                                                                 >  2:597923/1‑213 (MQ=21)                                                                                                                     tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACttttt                                                                                                                 <  1:597924/215‑3 (MQ=25)                                                                                                                                   tccAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATCCATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACg                                                                                                                                 <  1:823046/183‑1 (MQ=11)                                                                                                                                             gCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGt                                                                                                                               >  1:116690/1‑177 (MQ=32)                                                                                                                                             gCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGGGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGACGATGGGAGCCGACGGt                                                                                                                               >  1:30286/1‑177 (MQ=14)                                                                                                                                                    gCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGt                                                                                                                               >  1:910579/1‑170 (MQ=32)                                                                                                                                                         ttCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGtttt                                                                                                                            <  2:173915/168‑1 (MQ=33)                                                                                                                                                             ggaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGAc                                                                                                                                  <  2:161649/158‑1 (MQ=31)                                                                                                                                                              gaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGa                                                                          <  2:653867/213‑1 (MQ=33)                                                                                                                                                                        gcAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGGGaa                                                                         >  1:1107809/1‑204 (MQ=17)                                                                                                                                                                         cAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGa                                                                          <  1:498849/202‑1 (MQ=33)                                                                                                                                                                                ggCGGAGGGAGGTGACGGGCATGGAGGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCa                                                                                                              <  1:108877/158‑1 (MQ=18)                                                                                                                                                                                    gggggaggTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAgc                              <  1:1019963/233‑1 (MQ=37)                                                                                                                                                                                    gagggagaTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGaaa                                >  1:106529/1‑233 (MQ=25)                                                                                                                                                                                        cgcttGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACccgc                                                                                             >  1:415863/5‑170 (MQ=255)                                                                                                                                                                                         aggTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGcaca                         >  1:977610/1‑235 (MQ=37)                                                                                                                                                                                                    aTGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTg                                        >  1:836393/1‑209 (MQ=34)                                                                                                                                                                                                    aTGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAAcccaaaagcc              >  1:651737/1‑235 (MQ=37)                                                                                                                                                                                                     tGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAAcccaaaagccca            >  1:405183/1‑236 (MQ=37)                                                                                                                                                                                                                cAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAAcccaaaagcccaaaagccca    >  2:862964/1‑233 (MQ=39)                                                                                                                                                                                                                 aaGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAAcccaaaagcccaaaagcccaaa  >  2:899731/1‑234 (MQ=40)                                                                                                                                                                                                                  aGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAAcccaaaagcccaaaag        >  1:936981/1‑227 (MQ=37)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                    GCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACAACCCAAAAGCCCAAAAGCCCAAA  >  AE015451/2087504‑2087945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑