New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	3784356 =	61 (0.990)	3 (0.050)	3/326	NT	4.9%	coding (680/822 nt)	nikE	Nickel transporter ATP‑binding protein
	?	AE015451	3784455 =	58 (0.990)					coding (779/822 nt)	nikE	Nickel transporter ATP‑binding protein
Rejected: Frequency below/above cutoff threshold.

CTTGATCGGAGTCGGTCTTGGCTGAACAGGGTTATGCGCGCAGATCAGTCTGGTGAACATAGCCTGTTTTCCGACGATCCGAAAGCCAGTCTGTGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/3784596‑3784356 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcgcccgcAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGACTGATCTGCGCGCATAACCCTGTTCAGCCAAGACCGACTCCGATCAAGGAAACGATCCATGCAGTGACGTGAAATGCTGGAAACCGTCGCGGGTGCCGTAGCCTTGGCGATGGGCACATTGTCGTTACACGCCCAC  >  AE015451/3784455‑3784684                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         CTTGATCGGAGTCGGTCTTGGCTGAACAGGGTTATGCGAGCAGATCAGTCTGGTGAACATAGCCTGTTTTCCGACGATCCGAAAGCCAGTCTGTGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCG                                                                                                                                                                                                                                             >  1:105355/1‑236                                                         ACATAGCCTGTTTTCCGACGATCCGAAAGCCAGTCTGTGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCG                                                                                                                                                                                                                                         <  2:825950/184‑1                                                                     TTCCGACGATCCGAAAGCCAGTCTGTGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCACAGCACAGGGCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCG                                                                                                                                                                                                                                         >  2:304980/1‑172                                                                                           CTGTGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCG                                                                                                                                                                                                                                             >  2:632928/1‑146                                                                                            tgcGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCCCAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCG                                                                                                                                                                                                                                         <  2:897341/146‑1                                                                                                                                            TCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAAC                                                                                                                                                                                                         <  2:648500/133‑1                                                                                                                                                                      GCCTGCAGCTGCCGGCCCCCGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCGCAGATGAGGCCATCACCGCAAGGCCTGCCAACAGCTG                                                                                                                                                                                                    <  1:38956/112‑1                                                                                                                                                                                    GCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGCCTGATCTGCGCGCATAACCCTGTTCAGCCA                                                                                                            >  2:188354/1‑186                                                                                                                                                                                                                                            GCCCGCAGCGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCG                                                                                                                                                                         <  2:534066/69‑1                                                                                                                                                                                                                                            GCCCGCAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGACTGATCTGCGCGCATAACCCTGTTCAGCCAAGACCGACTCCGATCAAGGAAACGATCCATGCAGTGACGTGAAATGCTGGAAACCGTCGCGGGTGCCGTAGCCTTGGCGATGGGCACATTGTCGTTACACGCCCAC  >  1:591110/1‑236                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         CTTGATCGGAGTCGGTCTTGGCTGAACAGGGTTATGCGCGCAGATCAGTCTGGTGAACATAGCCTGTTTTCCGACGATCCGAAAGCCAGTCTGTGGTGTCAGTGTGCAGCTGTTGGCAGGCCTTGCGGTGATGGCCTCCTCTGCGGGCGCACAGGCAACACCGCCGCCTGCAGCTGCCGGCCCACGGGGTGCGCAAAGCACAGGTCGTGCGTGACGGGGCGTTCCTCCACGATGCGCCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/3784596‑3784356 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgcgcccgcAGAGGAGGCCATCACCGCAAGGCCTGCCAACAGCTGCACACTGACACCACAGACTGGCTTTCGGATCGTCGGAAAACAGGCTATGTTCACCAGACTGATCTGCGCGCATAACCCTGTTCAGCCAAGACCGACTCCGATCAAGGAAACGATCCATGCAGTGACGTGAAATGCTGGAAACCGTCGCGGGTGCCGTAGCCTTGGCGATGGGCACATTGTCGTTACACGCCCAC  >  AE015451/3784455‑3784684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.