New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5395583	78 (1.260)	4 (0.070)	4/330	NT	5.0%	coding (61/1470 nt)	hsdM	Type I restriction enzyme EcoEI M protein
	?	AE015451	= 5395587	78 (1.310)					coding (65/1470 nt)	hsdM	Type I restriction enzyme EcoEI M protein
Rejected: Frequency below/above cutoff threshold.

CTGGTTAAAGCCTTTGGCGGCAAAGCTGGCTACAACAAAGCTATCCATGAACTGGAAGACGCGCTGTACGCCAGTTAATCCGCTCTACCCGTTGGGTGGGCAACGGCGGAGCCTTGTCCACCTAAGCAGCCAACTCCATTACAATCCCCCGCTTTGCCACACCGATTGAGAATTCGTTATGTCCATCAGCTCCACCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGGCGTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5395345‑5395583 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgtcgCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGGCTCCGCCGTTGCCCACCCAACGGGTAGAGCGGATTAACTGGCGTACAGCGCGTCTTCCAGTTCATGGATAGCTTTGTTGTAGCCAGCTTTGCCGCCAA  <  AE015451/5395587‑5395358                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       CTGGTTAAAGCCTTTGGCGGCAAAGCTGGCTACAACAAAGCTATCCATGAACTGGAAGACGCGCTGTACGCCAGTTAATCCGCTCTACCCGTTGGGTGGGCAACGGCGGAGCCTTGTCCACCTAAGCAGCCAACTCCATTACAATCCCCCGCTTTGCCACACCGATTGAGAATTCGTTATGTCCATCAGCTCCACCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGG                                                                                                                                                                                                                                             >  2:585571/1‑234   GGTTAAAGCCTTTGGCGGCAAAGCTGGCTACAACAAAGCTATCCATGAACTGGAAGACGCGCTGTACGCCAGTTAATCCGCTCTACCCGTTGGGTGGGCAACGGCGGAGCCTTGTCCACCTAAGCAGCCAACTCCATTACAATCCCCCGCTTTGCCACACCGATTGAGAATTCGTTATGTCCATCAGCTCCACCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGGCGT                                                                                                                                                                                                                                          <  1:1094930/235‑1                                                                                                                                     ACTCCATTACAATCCCCCGCTTTGCCACACCGATTGAGAATTCGTTATGTCCATCAGCTCCACCATCAAGTCCATCCAGGACATCATGCGCCAAGACGTCGGCGTCGCCATCGACGCCGACGT                                                                                                                                                                                                                        >  1:1072958/1‑123                                                                                                                                                                        GAGAATTCGTTATGTCCATCAGCTCCACCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGGCGTCGCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTG                                                                                                                                                    >  2:818365/1‑156                                                                                                                                                                                                   CCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGGCGTCGCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGG                                                                                                    <  1:495990/177‑1                                                                                                                                                                                                   CCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGGCGTCGCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGG                                                                                                    >  2:495989/1‑177                                                                                                                                                                                                                                          GCGTCGCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGGCTCCGCCGTTGCCCACCCAACGGGTAGAGCGGATTAACTGGCGTACAGCGCGTCTTCCAGTTCATGGATAGCTTTGTTGTAGCCAGCTTTGCCGCCAA  >  1:435134/1‑236                                                                                                                                                                                                                                           CGTCGCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGGCTCCGCCGTTGCCCACCCAACGGGTAGAGCGGATTAACTGGCGTACAGCGCGTCTTCCAGTTCATGGATAGCTTTGTTGTAGCCAGCTTTGCCGCCA   >  2:485895/1‑234                                                                                                                                                                                                                                            GTCGCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGGCTCCGCCGTTGCCCACCCAACGGGTAGAGCGGATTAACTGGCGTACAGCGCGTCTTCCAGTTCATGGATAGCTTTGTTGTAGCCAGCTTTGCCGCCAA  >  2:1003309/1‑234                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       CTGGTTAAAGCCTTTGGCGGCAAAGCTGGCTACAACAAAGCTATCCATGAACTGGAAGACGCGCTGTACGCCAGTTAATCCGCTCTACCCGTTGGGTGGGCAACGGCGGAGCCTTGTCCACCTAAGCAGCCAACTCCATTACAATCCCCCGCTTTGCCACACCGATTGAGAATTCGTTATGTCCATCAGCTCCACCATCAAGTCCATCCAGGACATCATGCGCAAAGACGTCGGCGTCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/5395345‑5395583 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcgtcgCCATCGACGCCGACGTCTTTGCGCATGATGTCCTGGATGGACTTGATGGTGGAGCTGATGGACATAACGAATTCTCAATCGGTGTGGCAAAGCGGGGGATTGTAATGGAGTTGGCTGCTTAGGTGGACAAGGCTCCGCCGTTGCCCACCCAACGGGTAGAGCGGATTAACTGGCGTACAGCGCGTCTTCCAGTTCATGGATAGCTTTGTTGTAGCCAGCTTTGCCGCCAA  <  AE015451/5395587‑5395358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.