Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	1,531,732	Δ107 bp	29.3%	coding (38‑144/630 nt)	PP_1344 ←	conserved protein of unknown function

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1531731	6 (0.100)	7 (0.120)	7/330	NT	29.3%	coding (145/630 nt)	PP_1344	conserved protein of unknown function
	?	AE015451	1531839 =	28 (0.470)					coding (37/630 nt)	PP_1344	conserved protein of unknown function

GCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCGACTCATGGGGCCGCCCTCCATCCCTAAGTGTGGAAGATC  >  AE015451/1531612‑1532065                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   tcggtaaccaccagcaacaaagtaccatcgcgccaggacgccacatggcagtgctcgcgggcagccggttgcaactggctttccagcaggcgctgcaagtgctccaggcgctcggcctggttcagcagcaggcgcagcgggcgcacctggcgcagcagggcggaaggtggctgggcgggggagggtttgtaggccatgggggtacgcgtgaagtgacgtgggcacagGCAGCCaaa                                                                                                                                                                                                                            <  1:982243‑M2/9‑1 (MQ=255)                                                           gggcagccggttgcaactggctttccagcaggcgctgcaagtgctccaggcgctcggcctggttcagcagcaggcgcagcgggcgcacctggcgcagcagggcggaaggtggctgggcgggggagggtttgtaggccatgggggtacgcgtgaagtgacgtgggcacagGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGc                                                                                                                                                                  >  1:1105637‑M2/170‑236 (MQ=255)                                                                                        gggcgctgcaagtgctccaggcgctcggcctggttcagcagcaggcgcagcgggcgcacctggcgcagcagggcggaaggtggctgggcgggggagggtttgtaggccatgggggtacgcgtgaagtgacgtgggcacagGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATg                                                                                                                                                                   <  2:811739‑M2/66‑1 (MQ=255)                                                                                           gggggTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAgga                                                                                                                                                 >  1:343015/1‑220 (MQ=255)                                                                                                                                      ttGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCGCAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCa                                                                                                                                                                            >  2:505757/1‑151 (MQ=255)                                                                                                                                      ttGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTg                                                                                                                                                                                            >  2:773752/1‑135 (MQ=255)                                                                                                                                      ttGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAg                                                                                                                                                                                       >  1:43519/1‑140 (MQ=255)                                                                                                                                      ttGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGaa                                                                                                                                                                       >  1:565263/1‑156 (MQ=255)                                                                                                                                      ttGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTaaa                                                                                                                                                       >  1:273734/1‑172 (MQ=255)                                                                                                                                      ttGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTa                                                                                                                                                         >  2:387800/1‑170 (MQ=255)                                                                                                                                      gcagcgggcgcacctggcgcagcagggcggaaggtggctgggcgggggagggtttgtaggccatgggggtacgcgtgaagtgacgtgggcacagGCAGCCAAAGACAAGACTGGTTCTCGATCGCTTTCATGTTGCCACGCCTTGAACt                                                                                                                                                                              >  2:85887‑M2/95‑149 (MQ=255)                                                                                                                                       tGCCTGTGCCGGCCTCTTCGCGGGTGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGGTCTCGATAGCTCTCATGTTGTGAAGCCTGGaa                                                                                                                                                                                >  1:525427/1‑146 (MQ=25)                                                                                                                                            gtgCCGGCCTCTTCGCGGGTGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTc                                                                                                                                                                                                  >  2:280103/1‑123 (MQ=255)                                                                                                                                                      ctTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTa                                                                                >  2:790211/1‑227 (MQ=255)                                                                                                                                                       ttCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGCCCCCTGCCAAAACGCCGTATCAGCATCGTGGAAATCCACCACTCTCCTCACCATCGt                                                                                         >  2:453307/1‑217 (MQ=255)                                                                                                                                                          gcgGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGACCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCg                                                                   >  1:245525/1‑236 (MQ=255)                                                                                                                                                                gAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACg                                                                                                                                    >  1:187825/1‑165 (MQ=255)                                                                                                                                                                    ccGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTg                                                                                                                                            >  2:539282/1‑153 (MQ=255)                                                                                                                                                                      gCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTAt                                                       >  1:920935/1‑236 (MQ=255)                                                                                                                                                                                        ggTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGtt                                                                     >  2:1123872/1‑204 (MQ=255)                                                                                                                                                                                         gTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCg                                         >  2:397496/1‑231 (MQ=255)                                                                                                                                                                                               tGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGc                                                                                                                                           >  1:158895/1‑127 (MQ=255)                                                                                                                                                                                               tGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGc                                                                                                                                           <  2:158895/127‑1 (MQ=255)                                                                                                                                                                                               tGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCg                                                                   >  2:815199/1‑199 (MQ=255)                                                                                                                                                                                               tGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCg                                                                   <  1:815200/199‑1 (MQ=255)                                                                                                                                                                                                       cGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAAAGCt                                                                                                                                  >  2:251856/1‑128 (MQ=255)                                                                                                                                                                                                         ggTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAAc                                                                                                                                                                               <  2:568024/81‑1 (MQ=255)                                                                                                                                                                                                          gTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCGACTCATGGGgcc                             >  1:796630/1‑226 (MQ=255)                                                                                                                                                                                                           ttCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGc                                                   >  1:140923/1‑203 (MQ=255)                                                                                                                                                                                                              gcgtgaagtgacgtgggcacagGCAGCCAGAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGc                                                                                                                                           >  2:175636‑M2/23‑112 (MQ=255)                                                                                                                                                                                                               cgtgaagtgacgtgggcacagGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCGACTCATGGGGCCGCCCTCCATCCCTa               >  1:585079‑M2/22‑235 (MQ=255)                                                                                                                                                                                                                  gcgAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGc                                                   >  2:927964/1‑196 (MQ=255)                                                                                                                                                                                                                     gtgacgtgggcacagGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACggg                                           <  2:1105636‑M2/186‑1 (MQ=255)                                                                                                                                                                                                                      agagGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGACATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCCCa                                                                 >  2:537901/1‑178 (MQ=255)                                                                                                                                                                                                                       gagGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCGACTCATGGGGCCGCCCTCCAt                    >  2:171765/1‑222 (MQ=255)                                                                                                                                                                                                                        agGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTAt                                                       >  1:1113545/1‑186 (MQ=255)                                                                                                                                                                                                                           tgggcacagGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCGACTCATGGGGCCGCCCTCCATCCCTAAGTGTGGAAGATc  >  1:35035‑M2/10‑236 (MQ=255)                                                                                                                                                                                                                              gcacagGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCt                                                  <  2:830366‑M2/179‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   GCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTAGGAGCGGGTTCACCCGCGAAGAGGCCGGCACAGGCAGCCAAAGACAAGACTGGTTCTCGATAGCTTTCATGTTGCCAAGCCTTGAACTCAGTGAAAATGCCCCTATCTAAAACAGGCCCCTGCCAAAACGCTGTATCAGCATCGTGGAAATCCACCACTTTCCTCACCATCGTTTCCGGGTAGAATGCTCGTTCGCATGCGGCCTATGGGCTGCACGGGCGACTCATGGGGCCGCCCTCCATCCCTAAGTGTGGAAGATC  >  AE015451/1531612‑1532065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.