| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| JC | AE015451 | 4,980,585 | +GGC | 100% | intergenic (‑24/+44) | PP_4387 ← / ← flgE | conserved exported protein of unknown function/Flagellar hook protein FlgE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AE015451 | = 4980585 | 0 (0.000) | 33 (0.540) +GGC |
28/338 | NT | 100% | intergenic (‑24/+44) | PP_4387/flgE | conserved exported protein of unknown function/Flagellar hook protein FlgE |
| ? | AE015451 | 4980586 = | 0 (0.000) | intergenic (‑25/+43) | PP_4387/flgE | conserved exported protein of unknown function/Flagellar hook protein FlgE | |||||
CGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCG > AE015451/4980378‑4980798 | ggcaggcggcgttgttgtaggggccagagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCg > 1:391873‑M2/209‑217 (MQ=255) taagagacaggaggggcgcgcccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAatcat < 1:329241‑M2/44‑1 (MQ=255) agagtggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCtt > 1:315654‑M2/184‑188 (MQ=255) tggcgcacccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGaa < 1:1129974‑M2/57‑1 (MQ=255) acccagcctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCa > 2:1031356‑M2/174‑201 (MQ=255) cctttgcccgggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAac < 2:1128768‑M2/24‑1 (MQ=255) ggttgtgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAatc < 1:122853‑M2/42‑1 (MQ=255) tgggccgaacacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCa < 2:529327‑M2/34‑1 (MQ=255) cacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATgg > 2:427218‑M2/144‑206 (MQ=255) cacaggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATgg < 1:430735‑M2/93‑1 (MQ=255) aggtagcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAat < 1:31322‑M2/41‑1 (MQ=255) gcggccggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTgcatgga > 2:953193‑M2/136‑206 (MQ=255) cggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCt > 1:516280‑M2/131‑196 (MQ=255) cggtgaccaggctttgccattgatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGc > 1:952561‑M2/131‑236 (MQ=255) gatagaacggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCt < 1:616716‑M2/126‑1 (MQ=255) ggggcgggggcagcgggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacaacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGc < 2:466114‑M2/68‑1 (MQ=255) ggggcaggcagaaccagcaccagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCAtt < 2:190986‑M2/103‑1 (MQ=255) cagcaaggggaacaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCGTTg > 2:971425‑M2/67‑73 (MQ=255) acaaggccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggaCGc > 2:21679‑M2/56‑58 (MQ=255) ccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAac < 1:963691‑M2/24‑1 (MQ=255) ccagtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAac > 2:963690‑M2/50‑73 (MQ=255) gtaacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGc > 1:510262‑M2/47‑224 (MQ=255) taacgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATc > 1:651539‑M2/46‑236 (MQ=255) acgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAg > 2:542586‑M2/44‑74 (MQ=255) acgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAg < 1:542587‑M2/31‑1 (MQ=255) acgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTg > 2:1074491‑M2/44‑159 (MQ=255) acgacagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTgc > 2:895549‑M2/44‑165 (MQ=255) acagccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTg < 2:651538‑M2/193‑1 (MQ=255) gccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCt < 2:1016392‑M2/79‑1 (MQ=255) gccgcttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCt > 1:1016393‑M2/38‑116 (MQ=255) cttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTg > 1:913383‑M2/34‑119 (MQ=255) cttcattcgcgtgaaccttcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTg < 2:913382‑M2/86‑1 (MQ=255) tcacattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCATGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCg > 1:1031658‑M2/16‑228 (MQ=255) cattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGGCTTGATCAGGTTTa > 1:901956‑M2/13‑150 (MQ=255) cattcttggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTAGCCGGTCAGGTCGACGTTGGAGtcc > 2:678047‑M2/13‑182 (MQ=255) tggggcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTGGATCAGGGTTACCAGCTCGGCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGCAATCTGGCCGAGGGTAccgggg > 2:123745‑M2/7‑213 (MQ=255) gcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCAt > 2:291980‑M2/3‑54 (MQ=255) gcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCt > 2:413442‑M2/3‑128 (MQ=255) gcCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCt < 1:413443‑M2/126‑1 (MQ=255) | CGCGGCAGGCGGCGTTGTTGTAGGGGCCAGAGTGGCGCACCCAGCCTTTGCCCGGGTTGTGGGCCGAACACAGGTAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCAGCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGCGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAGCTCGCCGGTCAGGTCGACGTTGGAGTCCTCCAGGGCACCACCGGAAATCTGGCCGAGGGTACCGGTGTCCG > AE015451/4980378‑4980798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |