Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	2,087,690	G→A	23.1%	intergenic (+503/+415)	PP_1865 → / ← aroH	transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	2,087,690	0	G	A	23.1%	68.1 / 21.5	39	intergenic (+503/+415)	PP_1865/aroH	transposase/Phospho‑2‑dehydro‑3‑deoxyheptonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (15/15);  new base A (5/4);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01

AGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACA  >  AE015451/2087465‑2087922                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           aGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCAt                                                                                                                                                                                                                                  >  1:653868/1‑236 (MQ=255) aGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGc                                                                                                                                                                                                                                    >  2:805890/1‑234 (MQ=255)              aTCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTc                                                                                                                                                                                                                       >  2:405957/1‑234 (MQ=255)                                        gCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGcc                                                                                                                                                                                            <  1:405958/235‑1 (MQ=21)                                        gCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCt                                                                                                                                                                                           <  1:805891/236‑1 (MQ=21)                                                     cGGTCATCTGGATCAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTCCCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAGGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCaa                                                                                                                                                                                                                     <  1:131437/197‑1 (MQ=255)                                                                            ccGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCa                                                                                                                                                                                                                      >  2:664256/1‑173 (MQ=255)                                                                            ccGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgggg                                                                                                                                                                       >  1:217197/1‑217 (MQ=255)                                                                             cGGCATGGCTGCCCTCGTCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCGTTTGCTTCTACGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCacctccctc                                                                                                                                                                                                              <  1:59371/180‑9 (MQ=255)                                                                                   ggCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGGTGCACGTc                                                                                                                                                                                                                       >  2:648543/1‑165 (MQ=255)                                                                                            cGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTg                                                                                                                                                                                                               >  1:637456/1‑164 (MQ=18)                                                                                             ggCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTGCTCCCGGGAGCAAGGCCGGAGCGAGGGGAcc                                                                                                                                                 >  1:716763/1‑224 (MQ=11)                                                                                             ggCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCATGGATGGGGCCTGCAGCGC‑GTGCTCCCGGGAGCAAGGCCGGAGCGAGGGGAcc                                                                                                                                                 <  2:716762/224‑1 (MQ=11)                                                                                                 tGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAgg                                                                                                                                <  1:231182/236‑1 (MQ=255)                                                                                                      tACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCCAGCGCTG‑GGCCCCA‑gg                                                                                                                                                                                                    >  1:647244/1‑163 (MQ=255)                                                                                                            gaagaaCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATGGATGCCCGTCAAGCGCTGAGGCCCCAGGGATGGGGCCTGCAGCGCggg                                                                                                                                                                                 >  2:125003/1‑177 (MQ=11)                                                                                                                 aCGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGa                                                                                                                 >  1:437419/1‑235 (MQ=255)                                                                                                                  ctccGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTACGCGCGCGGTATTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTg‑gg                                                                                                                                                                                                            <  1:56926/142‑1 (MQ=255)                                                                                                                     cGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCg                                                                                                             >  1:1032702/1‑235 (MQ=40)                                                                                                                      gTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGAc                                                                                                           >  1:384922/1‑236 (MQ=39)                                                                                                                                 tcttgctcttgctTTGGCTTTTGCTTCTAGGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgc                                                                                                                                                                                                                   <  1:1065955/123‑1 (MQ=25)                                                                                                                                        cttgctTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCgg                                                                                                                                                                         <  1:664257/156‑1 (MQ=31)                                                                                                                                              ttGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGGCGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCg                                                                                                                                              <  1:496446/177‑1 (MQ=14)                                                                                                                                              ttGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGGCAAGCGCTG‑GGCCCCA‑GGAAGGGGCCt                                                                                                                                                                                           >  2:919088/1‑132 (MQ=17)                                                                                                                                              ttGGCTTTTGCTTCTAAGCGCGCGGTAGTCCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGtttt                                                                                                     <  1:898839/218‑1 (MQ=21)                                                                                                                                                        cTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCTTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGcgtagcgtag                                                                                                                                 >  1:1074319/1‑180 (MQ=17)                                                                                                                                                            tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACttttt                                                                                          <  1:597924/215‑3 (MQ=25)                                                                                                                                                            tAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACttttt                                                                                          >  2:597923/1‑213 (MQ=21)                                                                                                                                                                cgcgcgGTAGTTCAGGCGACGCAGATTGCGCCTTCAGGAGGCGGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAgcg                                                                                                                                                                                                                  <  1:386116/93‑1 (MQ=9)                                                                                                                                                                          tccAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGTGGAGGCATTGCGGAGGGAGATGACGGGCATCCATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACg                                                                                                          <  1:823046/183‑1 (MQ=11)                                                                                                                                                                                    gCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGt                                                                                                        >  1:116690/1‑177 (MQ=32)                                                                                                                                                                                    gCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGGGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGACGATGGGAGCCGACGGt                                                                                                        >  1:30286/1‑177 (MQ=14)                                                                                                                                                                                           gCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGt                                                                                                        >  1:910579/1‑170 (MQ=32)                                                                                                                                                                                                ttCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGtttt                                                                                                     <  2:173915/168‑1 (MQ=33)                                                                                                                                                                                                    ggaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGAc                                                                                                           <  2:161649/158‑1 (MQ=31)                                                                                                                                                                                                     gaggCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGa                                                   <  2:653867/213‑1 (MQ=33)                                                                                                                                                                                                               gcAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGGGaa                                                  >  1:1107809/1‑204 (MQ=17)                                                                                                                                                                                                                cAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGa                                                   <  1:498849/202‑1 (MQ=33)                                                                                                                                                                                                                       ggCGGAGGGAGGTGACGGGCATGGAGGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCa                                                                                       <  1:108877/158‑1 (MQ=18)                                                                                                                                                                                                                           gggggaggTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAgc       <  1:1019963/233‑1 (MQ=37)                                                                                                                                                                                                                           gagggagaTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGaaa         >  1:106529/1‑233 (MQ=25)                                                                                                                                                                                                                                aggTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGcaca  >  1:977610/1‑235 (MQ=37)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                           AGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGTCATCTGGATAAGGATATCGCCGGCATGGCTGCCCTCGGCCTGGTGTACCTGGAAGAACGCCGTTGCTCTTGCTCTTGCTCTTGCTTTGGCTTTTGCTTCTAAGCGCGCGGTAGTTCAGGCGACGCAGATTGCGACTTCAGGAGGCCGAGCGCAGGGCTTGCGGAGGGAGGTGACGGGCATGGATGCCCGTCAAGCGCTG‑GGCCCCA‑GGATGGGGCCTGCAGCGCGGTCCTCCCGGGAGCAAGCCCGGAGTGAGGGGACCCCGGAGCGTAGCGTAGGGGCCGGATGATGGGAGCCGACGGTTTTGGTTACTTTGGCCACGACCAAAGTAACCCGCCGGAAGGGCGGAAAGGTGAATAGGCGCCTACATCGTGAATGAATGTCTACTTGATGCGAAAGCGCACA  >  AE015451/2087465‑2087922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑