Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	3,951,161	A→T	100%	intergenic (‑125/+70)	PP_3486 ← / ← PP_3487	putative cytochrome c/conserved exported protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	3,951,161	0	A	T	100.0%	171.9 / NA	46	intergenic (‑125/+70)	PP_3486/PP_3487	putative cytochrome c/conserved exported protein of unknown function
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (31/15);  total (31/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCC  >  AE015451/3950935‑3951383                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               cAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACg                                                                                                                                                                                                                        <  1:1045644/235‑1 (MQ=255)                  tGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACaaa                                                                                                                                                                                                      <  1:944183/236‑1 (MQ=255)                            cTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGCAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAg                                                                                                                                                                                                                    >  1:1096042/1‑212 (MQ=255)                                        gCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGc                                                                                                                                                                                                                       <  1:894124/197‑1 (MQ=255)                                                       ttCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGc                                                                                                                                                                                                                       <  1:1003249/182‑1 (MQ=255)                                                                              ccagggcTGAGGTCGAAGTTGCCAGGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCGTTTCGCGACGCATGGCCGCTCCcac                                                                                                                                                                                                         >  2:615822/1‑173 (MQ=255)                                                                                    cTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCca                                                                                                                                                                                           >  2:905205/1‑181 (MQ=255)                                                                                    cTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGGGGCAGCTCAAGCGCAGAAAGAGGGGAGAGCTGCGCGCTCCTTTCGCGAc                                                                                                                                                                                                                         >  1:10573/1‑151 (MQ=255)                                                                                          tCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCCCaaa                                                                                                                                                                                                      >  1:167274/1‑164 (MQ=255)                                                                                               gTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCg                                                                                                                                          <  1:617452/219‑1 (MQ=255)                                                                                                                     tAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCAGGGCAATcgccg                                                                                                      >  1:328401/1‑233 (MQ=255)                                                                                                                                gCAAGCCCTGGGGGAGCGGCCTCGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAggc                                                                                                                                                                       <  2:333217/157‑1 (MQ=255)                                                                                                                                 cAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGc                                                                                                                                        >  1:589391/1‑187 (MQ=255)                                                                                                                                      ccTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCCGGCGGTGCa                                                                                           >  1:967278/1‑227 (MQ=255)                                                                                                                                             gAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTcccc                                                                                                                                                                           >  1:664514/1‑140 (MQ=255)                                                                                                                                                  gCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGt                                                                                              <  2:688970/212‑1 (MQ=255)                                                                                                                                                     ttGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACCGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCCGGGTCAACAGCTGCATGCGCCggg                                                                                                               >  1:44414/1‑192 (MQ=255)                                                                                                                                                     ttGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCTGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGCGCCTCCGGGCGAAt                                                                                >  1:206351/1‑223 (MQ=255)                                                                                                                                                     ttGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGc                                                                                            >  1:591776/1‑211 (MQ=255)                                                                                                                                                       gCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAg                                                                                                                                                                         >  2:520664/1‑132 (MQ=255)                                                                                                                                                       gCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTg                                                                                             >  1:195609/1‑208 (MQ=255)                                                                                                                                                          tCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAACGCGGATGCcacc                                                                                                                                                                                         >  2:223128/1‑112 (MQ=255)                                                                                                                                                            gcgAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCt                                                             <  1:798614/235‑1 (MQ=255)                                                                                                                                                             cgAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGc                                                                                            <  1:905206/203‑1 (MQ=255)                                                                                                                                                                aaGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGCTACTCACCGACCGGCCAGCGGCTGGGTCAACAGCt                                                                                                                           >  2:1031065/1‑169 (MQ=255)                                                                                                                                                                aaGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAAc                                                                                                                               >  1:425136/1‑165 (MQ=255)                                                                                                                                                                aaGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCACTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCt                                                                          >  1:627343/1‑218 (MQ=255)                                                                                                                                                                aaGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACCCACCGACCGGCCAGCGGCTGGGTCAACAg                                                                                                                             >  2:886082/1‑167 (MQ=255)                                                                                                                                                                     cTGCGCAGCAGCCCCGGCAGTCGCAGCTCAAGCGCAGAAAGAGGGGAGCGCCGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCcaca                                                                                                                                                                                         <  2:451550/102‑1 (MQ=38)                                                                                                                                                                        cgcAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGt                                                                   >  2:986982/1‑217 (MQ=255)                                                                                                                                                                        cgcAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCg                                                 <  1:780582/235‑1 (MQ=255)                                                                                                                                                                           agcagcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATTCGCCTGGCAATCGCCGCCgg                                                                                                  >  2:286986/1‑183 (MQ=255)                                                                                                                                                                                ccccGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATcc                                                                                        <  1:149341/188‑1 (MQ=255)                                                                                                                                                                                ccccGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATc                                           >  2:1040662/1‑233 (MQ=255)                                                                                                                                                                                  ccGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCCTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTcccc                                                                                                                                                                           <  1:600712/103‑1 (MQ=255)                                                                                                                                                                                   cGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGa                                                                                  >  2:906562/1‑191 (MQ=255)                                                                                                                                                                                       aGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCCCTCCTTCCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCacc                                                                                                                                                      <  2:664513/119‑1 (MQ=39)                                                                                                                                                                                            cAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTc                                                                                                                                  >  2:149341/1‑134 (MQ=255)                                                                                                                                                                                             aGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTc                                                                         >  2:493517/1‑190 (MQ=255)                                                                                                                                                                                             aGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAAc                                                        >  1:12820/1‑207 (MQ=255)                                                                                                                                                                                                         gAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACAt                                                      <  1:205768/197‑1 (MQ=255)                                                                                                                                                                                                                     cgcTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGCATCTTGCTCCAGCGTTTACCTGc                                                           >  1:225163/1‑180 (MQ=255)                                                                                                                                                                                                                       cTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCCTCCGGGCGAATCTTGCTCCAGCGTTTACCTGCCACATCACCGGCAATCAGCTGCg                                    >  1:185555/1‑201 (MQ=255)                                                                                                                                                                                                                       cTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGccg                                      >  2:710518/1‑197 (MQ=255)                                                                                                                                                                                                                       cTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGccc  >  1:512667/1‑235 (MQ=255)                                                                                                                                                                                                                             gcTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCaa                                                         >  1:872937/1‑174 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               CAATTGAGCGTCGCTACTGGAGACGCCCTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGCGCTGGGCAATCGCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCC  >  AE015451/3950935‑3951383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑