| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 5,988,844 | C→G | 33.3% | intergenic (+138/‑408) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 5,988,844 | 0 | C | G | 33.3% | 49.9 / 35.5 | 33 | intergenic (+138/‑408) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base C (7/15); new base G (3/8); total (10/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
CCCAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATC > AE015451/5988609‑5989072 | cccAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGgc < 1:223449/236‑1 (MQ=255) tgaCCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATcgcg < 1:891672/235‑1 (MQ=255) gtgtttggcgggaggttttcagcgcctgggaggtgttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 1:562563‑M2/123‑1 (MQ=255) gCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 1:1118235/235‑1 (MQ=255) gggagcGGTGGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTgg < 1:843150/218‑1 (MQ=255) ttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTaca > 2:1013476‑M2/79‑183 (MQ=255) ttcgccgggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 1:1098194‑M2/123‑1 (MQ=255) tGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgcg < 2:1031696/201‑1 (MQ=255) aTTCGGCTGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTa < 1:487629/234‑1 (MQ=255) gggggattttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAg < 1:1013477‑M2/159‑1 (MQ=255) gggtGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc < 1:477333/189‑1 (MQ=255) ttcagcgcctgggaggtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 2:799278‑M2/123‑1 (MQ=255) gAGGCCGTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACg < 2:984018/145‑1 (MQ=255) ccTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCGCGCGAGCCCCCTGGCGCACg < 2:339862/141‑1 (MQ=255) ggCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTg < 1:770800/178‑1 (MQ=255) gCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTCCGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATc < 1:237073/138‑1 (MQ=255) gCCTTGAGGGTTGTAGTGCCTGTGAGATTAGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 1:317833/161‑1 (MQ=255) ttcagcgCCTGGGAGATCGAGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑Gaaacaa < 1:952802/179‑1 (MQ=2) tgggagatcgagcgccgcccgcgCGCCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTg < 1:391296‑M2/128‑1 (MQ=255) aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATc > 1:264607/1‑112 (MQ=255) aTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATc < 2:264606/112‑1 (MQ=255) cTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCg < 2:890695/85‑1 (MQ=255) ccTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGaa < 1:952/126‑1 (MQ=255) tACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACag > 2:29530/1‑108 (MQ=255) tCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAACAACTATCGCCAGGCa > 2:930578/1‑197 (MQ=255) tCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgc > 1:983013/1‑236 (MQ=255) gggCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgat < 2:904564‑M2/164‑32 (MQ=255) gggCCAATTATTCCTGCGGGATTTGCGCGCGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGGTTACCCGCGAAgcgc > 2:1058992/1‑144 (MQ=2) gatttgcgcgcgaccgCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaaacacctcaaaattccaacgccgtcccctcaa < 1:88567‑M2/198‑91 (MQ=255) gcgcgcGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaa > 1:453382‑M2/1‑108 (MQ=255) gcgcgcGACCGCCTTGGGGCATGTCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGACTGGTGCAGGAGGGACTGGCC‑GAAACAAACGTAGGAGCGGGTTTAcccgcgaagcgccgcgcgggcggcgctcgatctcacaggcgctgaaaatcctccggcaa < 2:453381‑M2/167‑60 (MQ=255) gcACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATGCTGCAATTTc > 1:910230/1‑194 (MQ=255) aCGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAAcc > 1:965376/1‑234 (MQ=255) cccTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATc > 1:708067/1‑235 (MQ=255) cgcACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTcgccg > 1:463318/1‑195 (MQ=255) | CCCAGGGGCAAGTGCGTGATGACCGGGCCAAGGTGATGCAGACGGCCAAGGAGGCGCGGGTGGAGCTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGCGCCTGGCACAGGAGGGATTGGCCAGAAACAAACGTAGGAGCGGGTTTACCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCATGGGCGCCCAAAAACTATCGCCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATC > AE015451/5988609‑5989072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |