| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,318 | T→G | 11.0% | T1608T (ACT→ACG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,318 | 0 | T | G | 11.0% | 205.5 / 7.2 | 80 | T1608T (ACT→ACG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (30/39); new base G (3/6); total (35/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
TCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGA > AE015451/199083‑199521 | tCACTAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACt < 1:661786/236‑1 (MQ=14) ttCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCCGGCAACGAAGGCGACATCGt > 1:263650/1‑236 (MQ=18) ttCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGt > 1:611411/1‑236 (MQ=35) gATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAg > 2:685352/1‑228 (MQ=34) gATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAg < 1:685353/228‑1 (MQ=34) tactGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCt < 1:781466/235‑1 (MQ=34) gtGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCaa > 2:858840/1‑191 (MQ=33) gtGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGCCACCATCACGGCTGACCAGACTTc > 1:242219/1‑236 (MQ=25) tGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACGTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACtt < 2:611410/234‑1 (MQ=37) gCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCAcc > 1:1014300/1‑213 (MQ=34) aGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACagc > 1:364259/1‑197 (MQ=21) ccAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGc < 1:85853/235‑1 (MQ=35) caccGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:957756/235‑1 (MQ=37) gTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:926480/230‑1 (MQ=37) cGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGa > 2:424365/1‑169 (MQ=33) cGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCAt > 2:46862/1‑193 (MQ=34) ggCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCt < 1:354851/236‑1 (MQ=37) ggCCACCGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:915434/226‑1 (MQ=38) aCTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCa > 2:736309/1‑190 (MQ=35) aCTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCg > 2:639325/1‑228 (MQ=14) aCTGCAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCg < 1:639326/228‑1 (MQ=9) ggTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAgg < 2:193651/164‑1 (MQ=18) cgccCCGGACAACGTCTATGGCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 2:524166/214‑1 (MQ=38) ccccGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGcc > 2:1044144/1‑217 (MQ=40) ccccGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGcc < 1:1044145/217‑1 (MQ=39) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCATCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAAcc > 1:628438/1‑235 (MQ=255) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGAccc > 1:177382/1‑184 (MQ=37) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACCCCAGGCaa > 1:1064393/1‑146 (MQ=17) gACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGAACt > 2:767804/1‑138 (MQ=11) caccGTCTATGTCGGCACCAACCAGCCGGTAGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGCGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGACGAGAACGTCAAGCCGACCTTCACCGTGCACGTCa < 1:872624/227‑1 (MQ=255) cTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGcg > 1:178739/1‑171 (MQ=25) tATGTCGGCACCACCCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTc < 1:385998/222‑1 (MQ=255) tATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGTAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGc < 1:100960/236‑1 (MQ=255) tCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGcc < 2:818202/185‑1 (MQ=39) tCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCa < 1:371259/207‑1 (MQ=38) tCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCg > 1:249827/1‑227 (MQ=255) cGGCACCAACCAACCGGTGGTCAATTCCATCGACGAGGTCAGCGGTACAGATGCATGGAAGTTCGAAAACCTGAACCTGCACAAAACCCCTGTTAACACCGATGTCACATACGAGCCAGGCCCTCCAGGCAACGAAGGCCACATc < 2:481296/145‑1 (MQ=11) cACCAACCTGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGACCCTGGACAAGACCCCTGTCAGCACCGAGGTCACCTACGAGCCAGGCACTCCACGCAACGAAGGCGACTTCGTCAAGGTCAccacca < 2:936344/158‑1 (MQ=11) accaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCg > 2:1020729/1‑181 (MQ=37) ccaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGc > 2:837157/1‑219 (MQ=18) ccaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCga > 1:146693/1‑181 (MQ=18) ccaaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGc < 1:837158/219‑1 (MQ=255) caaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACt < 2:898554/118‑1 (MQ=18) caaccaGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACt > 1:898555/1‑118 (MQ=33) cggCCGGTGGTGAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCa < 2:568938/194‑1 (MQ=255) caGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGACACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCAcc > 2:579874/1‑198 (MQ=39) gCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACtt < 2:5829/164‑1 (MQ=18) gtggtCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGAccc > 1:14077/1‑235 (MQ=38) tcgtCAACGCCATCGGCGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCAc < 2:177382/137‑1 (MQ=255) tCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGTACGTCAAGCCGACCTTCACCCTGCACGTCAACCAGc < 2:952103/204‑1 (MQ=255) cATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCAACGAGGTCACCGACGAGACAGGCACACCAGGCAACGAAGGCGAc > 1:76457/1‑115 (MQ=255) aCGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGc > 2:894246/1‑165 (MQ=39) cgGTCAGCGGCGCAGAGGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTg < 2:529671/212‑1 (MQ=39) ggTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg < 1:249538/200‑1 (MQ=38) cAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCg > 2:168500/1‑139 (MQ=37) cAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCa > 1:64130/1‑195 (MQ=38) cggcgCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa < 1:933196/236‑1 (MQ=255) ggcgCAGAGGCAGGGAAGTCCGAAAGCCTGACCCTGGACAAGACCCCGGTCAGCGCCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCAcc < 2:241644/165‑1 (MQ=14) cgcAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaaca > 2:933195/1‑215 (MQ=255) aTGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGc < 2:1064392/177‑1 (MQ=39) aaGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCCACAACGCCCAGGTCACCATCCAGGCTGGTga > 1:32387/1‑230 (MQ=255) aaGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:517607/135‑1 (MQ=21) aGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg < 1:1105387/183‑1 (MQ=255) cGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAgcgc < 1:120824/234‑1 (MQ=40) gaaactctgaacctgGAAAAGACCCCGGTCACCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACCTCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCa < 2:1016008/136‑1 (MQ=11) gAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGc > 2:463921/1‑200 (MQ=37) aaaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa > 1:785776/1‑213 (MQ=38) aaaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTgaga < 1:696965/225‑1 (MQ=40) aaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca > 1:404290/1‑191 (MQ=37) aaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca < 2:404289/191‑1 (MQ=37) aaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGACAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCga > 2:676818/1‑120 (MQ=25) tgaacctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaaca < 2:412757/189‑1 (MQ=35) cctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTg > 2:519790/1‑178 (MQ=35) ggTCAGCACCGAGGTCATCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCTCGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaacaat < 2:584413/172‑2 (MQ=21) ggTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac < 2:1107459/172‑1 (MQ=35) tCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCCGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGa > 1:547247/1‑235 (MQ=255) cAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGa < 2:842363/144‑1 (MQ=255) aGCGCCGAGGTCACCGACGCGCCAGGCACGCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATAACGGCTgg < 2:239956/74‑2 (MQ=11) cACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTga < 2:628437/192‑1 (MQ=255) acccGAGGTCACCGACGGGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCAc < 2:297040/109‑1 (MQ=255) ggggTCACCGACGAGCCAGGTACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGAt < 2:547246/223‑1 (MQ=255) aCCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 2:288877/87‑1 (MQ=38) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAATGCTGGTGAGACCAGCGCGCCGTACACCCacga > 2:812639/1‑203 (MQ=255) acgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCCCCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAg < 2:420175/184‑1 (MQ=255) | TCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACCAGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGA > AE015451/199083‑199521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |