| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 210,088 | A→G | 6.3% | E5198E (GAA→GAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 210,088 | 0 | A | G | 6.3% | 175.9 / 2.8 | 63 | E5198E (GAA→GAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (21/38); new base G (2/2); total (23/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01 | |||||||||||
GTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAC > AE015451/209860‑210306 | gTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAgcg < 1:925982/236‑1 (MQ=31)gTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAg < 1:993249/234‑1 (MQ=31) tGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCt < 1:1068407/236‑1 (MQ=32) gACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTgc < 1:686297/236‑1 (MQ=33) gACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTgc < 1:645600/236‑1 (MQ=33) aaGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTgcg > 1:170685/1‑234 (MQ=32) aaGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCa > 1:315596/1‑236 (MQ=32) aaGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGCCACCATCAAGGCTGGCGAAACCAgca > 1:253081/1‑213 (MQ=14) cGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACAccc < 1:935417/222‑1 (MQ=32) tCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGtg < 1:659759/231‑1 (MQ=32) gTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAACCCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACa > 1:884855/1‑236 (MQ=18) tCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTa < 1:927004/233‑1 (MQ=33) gaGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGcc < 1:517341/235‑1 (MQ=33) gCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAg > 1:512754/1‑235 (MQ=33) aCGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGc < 2:1108114/225‑1 (MQ=33) cGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGTGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTggg < 1:807340/235‑1 (MQ=18) gCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCAt > 2:690364/1‑235 (MQ=11) gAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGc < 2:59402/209‑1 (MQ=32) aGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTcg < 1:564698/235‑1 (MQ=37) gACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTcggcg < 2:534574/234‑1 (MQ=35) aCCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCtt < 1:652707/236‑1 (MQ=35) cgccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCa < 2:170685/191‑1 (MQ=31) aCCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTg > 1:576028/1‑219 (MQ=34) ccAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGaa > 1:613751/1‑234 (MQ=14) agcccTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTcg < 1:147767/197‑1 (MQ=21) agaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCg > 1:955158/1‑236 (MQ=33) cGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACGCCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCt < 1:152341/204‑1 (MQ=14) cGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCt > 1:286904/1‑161 (MQ=31) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTggg < 2:825858/148‑1 (MQ=32) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTggg > 1:825859/1‑148 (MQ=32) cGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGt < 1:1101387/162‑1 (MQ=17) tCAACCAGCCGCTGGCCCACGACCTGGTCGTGACTCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTGCACCCACTCTGCGCAGAGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGt < 1:121820/220‑1 (MQ=19) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCt < 1:186825/151‑1 (MQ=31) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACCCCAc > 2:96979/1‑167 (MQ=14) cAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTggg < 2:633226/140‑1 (MQ=32) gCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTg > 1:152964/1‑164 (MQ=31) gCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGcgg > 1:283157/1‑188 (MQ=14) cttGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCAcc < 1:959525/140‑1 (MQ=11) cACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGGACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTACGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGcc > 1:783517/1‑191 (MQ=16) aCCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCGGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGcc < 1:325770/227‑1 (MQ=12) ccTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTgg > 2:235405/1‑140 (MQ=32) tCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCgaga < 1:536048/159‑1 (MQ=32) aGCCACAACGCACAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGtt < 1:262038/177‑1 (MQ=25) gCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGc > 2:657261/1‑127 (MQ=32) gCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGCCGGTGGACGCCACTGGTGCGAcc > 2:1013221/1‑140 (MQ=17) caacaaCGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCg > 2:604889/1‑161 (MQ=31) caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGa > 2:931521/1‑153 (MQ=11) caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGa < 1:931522/153‑1 (MQ=14) aacaacGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCg > 2:559378/1‑160 (MQ=14) aacaacGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTg < 2:193572/133‑1 (MQ=32) caacGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTg < 2:969646/215‑1 (MQ=38) cGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGCCGCCAc > 2:228704/1‑123 (MQ=11) ccAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAggcggc < 1:450764/235‑1 (MQ=255) cAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAggcggc < 2:703243/234‑1 (MQ=255) agctCACCATCAAGGCTGGCGAAGCCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAggcggc < 1:739200/230‑1 (MQ=255) aGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGCGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGGTCAAGTGAcc > 2:270074/1‑173 (MQ=25) tcgccATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATc < 1:230404/233‑1 (MQ=255) aCCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc < 1:550157/236‑1 (MQ=255) ccATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc < 1:986873/235‑1 (MQ=255) cAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGcc < 1:57507/187‑1 (MQ=37) ggCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGACGGTCGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAc < 1:633460/228‑1 (MQ=255) cTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGCCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCCAGATCAc < 1:959098/226‑1 (MQ=255) gCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAgtggt < 1:624112/175‑1 (MQ=18) aaaCCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGGGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCgag > 1:279377/1‑215 (MQ=255) | GTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCAC > AE015451/209860‑210306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |