| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 210,343 | G→A | 8.6% | P5283P (CCG→CCA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 210,343 | 0 | G | A | 8.6% | 272.8 / 7.9 | 90 | P5283P (CCG→CCA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (45/37); new base A (6/2); total (51/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCAC > AE015451/210110‑210558 | gCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGAt < 1:684971/236‑1 (MQ=255) ggTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATcaacaa < 2:576087/233‑1 (MQ=255) ggTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACca > 1:842352/1‑236 (MQ=255) gaCGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCCCCGACGAAGTGGTGGCCAAGCTGACTGCGCCCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGc < 1:255523/236‑1 (MQ=255) cGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCAt > 1:1116399/1‑236 (MQ=255) tACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGAc < 1:420431/236‑1 (MQ=255) tACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGAc < 1:188571/236‑1 (MQ=255) aCAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGCACGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCAATCAACAACCACAGTGCATTGAc > 1:859223/1‑235 (MQ=255) ccAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGc > 1:857512/1‑235 (MQ=255) aTCCGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCATCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATc < 2:512753/198‑1 (MQ=255) cTGGGCATCCCCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAAc < 1:235406/236‑1 (MQ=255) gggCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGCTCAACAACCACAGTg > 2:732683/1‑203 (MQ=255) cTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACTGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCt > 1:1107835/1‑203 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCGCAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCg > 1:67380/1‑236 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCg > 1:413045/1‑222 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGcc > 1:630626/1‑235 (MQ=255) ggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaa < 1:1100662/216‑1 (MQ=255) ggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaa > 2:1100661/1‑216 (MQ=255) aCGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAAc > 2:577195/1‑236 (MQ=12) aCGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAACACCGTCATCACCGTGCCGGCCaa > 1:625448/1‑235 (MQ=255) gCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACg > 1:553691/1‑235 (MQ=255) cTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCAcc < 1:888523/236‑1 (MQ=255) ggtgacaCCTTCGAGAACCTGGAGCTGGGCGGTGCCGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCaacaac < 1:800763/162‑1 (MQ=255) ggtcgcaCCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACg < 2:1111745/193‑1 (MQ=255) gTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCAcc < 2:553690/233‑1 (MQ=255) cGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGc < 1:96979/236‑1 (MQ=255) cGACCGTCGAGAGCCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCg < 2:1056260/218‑1 (MQ=255) ccTTCGAGAACCTGGAGCTGGGCGGCGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCAATCAACAACCACAGTGCATTGAcc > 1:619209/1‑176 (MQ=9) ctccGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAAcc < 2:152964/158‑1 (MQ=255) ttCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGTCCTGCCGATCAACAACCACAGTGCATTGACCTTCa > 1:568907/1‑178 (MQ=255) aaCCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGt > 1:761249/1‑236 (MQ=255) aaCCTGGAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCActg < 1:270075/235‑3 (MQ=255) cgAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTAGTGGCCAAGCTGACTGCGACCCCGTCGGTGACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGG‑‑CTGCCACCGTCACTGcc > 1:301002/2‑236 (MQ=11) gAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTg < 1:122983/235‑1 (MQ=255) cTGGGCGGTGCGGCTTCGGTTCAAGTGACCGCCACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAAc < 2:54956/204‑1 (MQ=255) cTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGcccc < 1:316891/236‑1 (MQ=255) ggCGGTGCGGCGTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCg < 2:991372/234‑1 (MQ=255) cgcggcgTCGGTCCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCGTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTCACTGCCCCGGACaat < 2:774870/234‑2 (MQ=11) ggCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTc > 1:841653/1‑235 (MQ=40) ggCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCAATCAACAACca > 1:1025332/1‑135 (MQ=18) ggCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCg > 2:843128/1‑200 (MQ=11) gcccGACACCCCCGACGAAGTGGTGGCCAACCTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGt < 2:280762/122‑1 (MQ=11) aCCGACACCCTCGACGAAGTGGTGGCCAAACTGACTGCCACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGc < 2:393274/180‑1 (MQ=255) aCCGACACCACCGACGAAGTGGTGGCCAAGCTGACGGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTg < 2:103552/205‑1 (MQ=255) tacacCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCAACACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGc < 2:126394/146‑1 (MQ=37) caccacCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCgc > 2:846587/1‑140 (MQ=37) caccacCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCCTCACCGTGCCGGCCAACGGCCACGTGGGCACt > 2:104221/1‑187 (MQ=255) caccacCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTg > 1:457993/1‑236 (MQ=255) gagacaggtggCCAAACTGACCGCCACCCCGTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGTAAGACCGTCATCACCGTGCCGGCCa < 1:600086/155‑1 (MQ=11) aGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAATAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGcccc < 1:942254/192‑1 (MQ=255) gtggCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGa > 2:722902/1‑226 (MQ=255) gtggCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATc < 2:955157/234‑1 (MQ=255) aaGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTGACTGCCCCGGACAACGCCCACgc > 2:133618/1‑196 (MQ=255) accgCGACCCCGTCCGTCACCGAAGGCGGCGAGATCACTTACACCAGCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCa < 2:198520/115‑1 (MQ=1) tGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCgg > 1:572579/1‑234 (MQ=255) ccccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGc < 2:315595/110‑1 (MQ=35) ccgccgGTCACCGAAGGCGGCGAGCTCACCTACACCATCACCCTGACCCACAAAGACGGTCTGCCGCTcc > 2:308927/1‑69 (MQ=11) ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGc > 1:480836/1‑108 (MQ=38) ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGc < 2:480835/108‑1 (MQ=38) ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACg > 2:301124/1‑181 (MQ=37) ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTc < 2:311362/183‑1 (MQ=37) ccGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTc > 1:311363/1‑183 (MQ=37) cGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCg > 2:883263/1‑201 (MQ=255) gacGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTTCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACTGTGCCGCCCAACGGCACTGTGGGCACt < 2:695373/146‑1 (MQ=255) cGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGt > 1:573339/1‑236 (MQ=255) gTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGc > 2:1081637/1‑125 (MQ=38) gTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGt > 2:1010054/1‑234 (MQ=255) gTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCAACCGt > 2:606148/1‑215 (MQ=255) cACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTa > 2:618228/1‑233 (MQ=255) gAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTg < 1:183659/114‑1 (MQ=17) gccagaTCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCCACTGTCACTGCCCCGGATAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGa < 2:946892/189‑1 (MQ=255) agaTCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGc < 1:536701/86‑1 (MQ=33) cACCTACACCATCACCCTGACCAACAAAGCCGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCGCGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCa < 2:48923/122‑1 (MQ=255) cccTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGCGCGACGTCAAGACCGTCATCACCGTGCCGGCCAACgtcc > 2:68496/1‑96 (MQ=255) cccTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGa > 2:70885/1‑130 (MQ=38) cccTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCg > 2:367460/1‑180 (MQ=255) cccTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCg > 2:1054960/1‑233 (MQ=255) cccTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTc > 1:191321/1‑236 (MQ=255) cccTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCg > 2:228358/1‑233 (MQ=255) cccTGACCAACAAAGACGGCCTGCCGATCAACAACAACAGCGCATTGACCTTCACGCTGAGCGACGGCCAGACCGTCATCACCGTGCCGGCCCACGGCACCGTGGGCa > 2:727255/1‑108 (MQ=11) cTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCa > 1:1024817/1‑235 (MQ=255) aacaaAGACGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCg < 2:577598/109‑1 (MQ=18) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGt < 2:507067/95‑1 (MQ=34) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGt > 1:507068/1‑95 (MQ=33) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGt > 2:480129/1‑128 (MQ=39) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCAc > 2:630522/1‑233 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGCGGTGCGGACGTCGGGAAGTTCGAGCAACTGACGCTg > 1:181515/1‑213 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGAcc > 2:969652/1‑221 (MQ=17) gCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTGGGCGGCGCGGACGTCGGTCAGTTCGAGCAACTGACGCTGGACAAGACGCCgg > 1:310314/1‑216 (MQ=255) cGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAAc > 1:342906/1‑128 (MQ=38) | GCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCAC > AE015451/210110‑210558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |