| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 213,918 | C→T | 10.4% | P6475L (CCG→CTG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 213,918 | 0 | C | T | 10.4% | 262.9 / 14.3 | 94 | P6475L (CCG→CTG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (41/41); new base T (5/4); total (48/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01 | |||||||||||
TCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAA > AE015451/213684‑214140 | tCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGGTCGAGCAACTGACACTCGCCAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGAACCCCAGGCAACGAAGGCGACCCg > 1:183453/1‑236 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCa > 1:950411/1‑236 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCa > 1:509359/1‑236 (MQ=255) gCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGCGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCa > 1:756961/1‑236 (MQ=255) ccGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCTGGTCAAg > 2:526376/1‑221 (MQ=255) gTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCAcggcc < 2:1006515/234‑1 (MQ=11) gTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCAcggcc < 1:808965/234‑1 (MQ=255) ccGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCTAGTCAAGGTCACCATCAccgcc < 2:574236/222‑1 (MQ=255) cGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgcc < 1:1111096/221‑1 (MQ=255) cGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAccgcc > 2:1111095/1‑221 (MQ=255) aaCGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGc > 2:674776/1‑233 (MQ=255) aaCGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCga > 1:390105/1‑236 (MQ=255) aaCGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCAACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGc > 1:706958/1‑233 (MQ=255) cGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:692095/235‑1 (MQ=255) gTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCa > 2:567171/1‑208 (MQ=255) cACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 2:390104/233‑1 (MQ=255) aCCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAc > 1:315430/1‑236 (MQ=255) ccGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGa > 2:686620/1‑201 (MQ=255) tGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCGGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCAGAGAACGTCAAACCGACCTTCACtgtg > 1:638731/1‑236 (MQ=255) aCTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACCCCAGGCCACGAAGGCGACCCGGTCAAGGt > 2:146091/1‑176 (MQ=255) gCTCCGGACAACGTCTACGTCGGTACCTACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAAcc < 1:1067803/218‑1 (MQ=255) gCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACg > 1:791240/1‑235 (MQ=255) cGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACAATCACCGCCGACCAGACCTCg > 2:493601/1‑194 (MQ=255) cGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGGCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCggc > 1:286197/1‑195 (MQ=255) aCGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCAc < 2:1072778/166‑1 (MQ=255) cGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACg < 1:330164/200‑1 (MQ=255) tACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCGACCGTTGAAGGCGCGGACGTTGGCAAGTTCGAGCAACTGACGCTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGa < 2:100852/205‑1 (MQ=255) cGGTACCAACGGCCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCgg < 2:323000/146‑1 (MQ=255) aCCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCg > 2:776400/1‑222 (MQ=255) ccAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTg > 1:142545/1‑236 (MQ=255) cAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAg > 1:649672/1‑166 (MQ=255) cAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGGCACCATCACCGCCGACCAGACCTCGGTGGCCcagaca > 2:284369/1‑182 (MQ=255) cAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACGGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGCCCTTCCCTGTGCACGt > 2:1128267/1‑210 (MQ=255) ggccatGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 2:308041/178‑1 (MQ=255) cTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGc < 2:950410/210‑1 (MQ=255) ntCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTCCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt < 2:872736/235‑1 (MQ=255) tCGTGATGTTGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt < 1:62554/235‑1 (MQ=255) tCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGa < 1:159920/220‑1 (MQ=255) cGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTAGcc > 1:863049/1‑166 (MQ=255) tGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 2:505798/170‑1 (MQ=255) cGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCtt > 2:910291/1‑174 (MQ=255) cGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGc < 1:88716/194‑1 (MQ=255) cGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACCACg > 2:485408/1‑233 (MQ=255) cGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGcc > 1:1096840/1‑235 (MQ=255) tACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg < 1:1040633/235‑1 (MQ=255) aaGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCGCCAt > 1:586019/1‑235 (MQ=255) aaGGAGCGGACGTTGGCAAGTTAGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTGCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCAcc > 2:213765/1‑116 (MQ=255) gCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCCGGCAACGAAGGCGACCGGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACCTCAAACCGa > 1:844093/1‑154 (MQ=255) gCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaac > 1:64198/1‑213 (MQ=255) ggACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGct > 1:515736/1‑234 (MQ=18) ggACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGc > 2:507994/1‑234 (MQ=255) gACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCACGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGACCGTCAACCCGACCtc > 2:360198/1‑154 (MQ=255) ccGTTGGCAAGTTCGAGCAGCTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:157100/142‑1 (MQ=255) cGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGAccc > 2:925521/1‑93 (MQ=255) cGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGAccc < 1:925522/93‑1 (MQ=255) cGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGcc > 2:842607/1‑214 (MQ=255) gCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGaaa < 2:707256/234‑1 (MQ=255) aaGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 1:646999/235‑1 (MQ=255) aGTTCGAGCAACTGACACTCTACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGGAACCAgc < 1:78204/236‑1 (MQ=255) aGTTCGAACAACTGACACGCGACAAGACCCCGGTCAGCACGTCGGTCACCGCCGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgc < 1:374256/236‑1 (MQ=255) aCTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGc > 1:218335/1‑187 (MQ=40) acacTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTACACGTCAACCAGCCGCTGGCCCACGAc > 2:716837/1‑165 (MQ=255) cgcGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 2:561732/215‑1 (MQ=255) cccGGTCAGCACTCCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCg < 1:174881/236‑1 (MQ=255) cccGGCCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGcc < 2:389272/213‑1 (MQ=255) ccGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGa > 1:1126993/1‑236 (MQ=255) cGGTCAGCACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCg < 1:619488/234‑1 (MQ=255) tCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACCACGCCCAGGTCAc > 2:743441/1‑181 (MQ=255) tCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGt < 2:649671/210‑1 (MQ=255) tCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCac > 1:470678/1‑218 (MQ=39) aGCACTTCGGTCACCGACGGGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCac > 2:151383/1‑181 (MQ=255) aCTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCGACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTa < 1:408009/236‑1 (MQ=255) ggTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTc < 1:247304/91‑1 (MQ=37) ggTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTa < 1:675859/231‑1 (MQ=255) cgacgaGCCGGGTACCCCAGGCAACGAAGGCGACCGGGTCAAGGTCACCATCCCCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTgg < 2:276123/134‑1 (MQ=255) cgacgaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACt < 2:43877/100‑1 (MQ=40) gacgaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCagg > 1:396079/1‑209 (MQ=255) gacgaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCagg < 2:396078/211‑3 (MQ=255) gacgaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCg > 2:561180/1‑214 (MQ=255) gaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAg < 1:918819/39‑1 (MQ=40) gaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTacac > 1:346062/1‑194 (MQ=38) gaGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGATGCTGGcc < 1:674777/235‑1 (MQ=255) aGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCg < 1:1097626/210‑1 (MQ=255) gCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGCGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTa > 1:514983/1‑189 (MQ=255) gCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCGCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcg < 1:360199/182‑1 (MQ=39) gCCGGGCACCCCGGGCACCGAAGGCGGCCCGGCCAAGGTCACCCCCACCGCCGACCAGACCTCGGTGGCCGAGAGCGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGc < 2:300013/117‑1 (MQ=14) ccGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCac > 1:966679/1‑195 (MQ=39) cGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATc < 1:561181/236‑1 (MQ=255) gggTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTAGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGGGACCTTgta > 1:270713/1‑136 (MQ=11) ccccAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAAcc < 2:64198/170‑1 (MQ=40) aGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgc < 1:524533/169‑1 (MQ=39) aCGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTg > 2:99225/1‑122 (MQ=38) aGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg < 2:592013/163‑1 (MQ=39) aGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg > 1:592014/1‑163 (MQ=39) gacGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCaa < 2:470677/228‑1 (MQ=255) gCGACCCGGTCAAGGTCACCCTCCCCGCCGGCCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGc < 2:142545/185‑1 (MQ=255) cccGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACgc > 1:477376/1‑188 (MQ=255) | TCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGTACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGTACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGAAGGTGCGGACGTTGGCAAGTTCGAGCAACTGACACTCGACAAGACCCCGGTCAGCACTTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCCGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAA > AE015451/213684‑214140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |