| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 215,383 | A→G | 6.9% | E6963E (GAA→GAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 215,383 | 0 | A | G | 6.9% | 261.5 / 8.5 | 100 | E6963E (GAA→GAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (28/64); new base G (2/5); total (30/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
CAAGTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATCACC > AE015451/215152‑215614 | cAAGTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 2:873731/236‑1 (MQ=31) aGTTCGACCAGCTGACACTCGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGCGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCa < 1:585160/234‑1 (MQ=9) gCTGGACAAGCCGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg < 1:93990/223‑1 (MQ=14) ggTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCCGCGCGCCATCCACCCa > 1:60450/1‑219 (MQ=17) tCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCa > 2:61109/1‑209 (MQ=31) ggTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGcc > 1:411908/1‑198 (MQ=31) gacgaGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCt < 1:784876/234‑1 (MQ=37) gCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAgcgcg < 2:710245/184‑1 (MQ=32) gCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGcc < 2:1077627/233‑1 (MQ=39) ccAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGa < 1:486151/235‑1 (MQ=37) cAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATc < 1:434791/236‑1 (MQ=38) gCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCt < 2:891911/190‑1 (MQ=34) gAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCAc < 2:971172/233‑1 (MQ=37) aaGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTc < 1:92252/235‑1 (MQ=37) aGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCg < 1:1006956/236‑1 (MQ=39) aCTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCGACGACCTGGTCGTGACCCTGAGCAACAACGCCCACGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCGCCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGAt > 2:698539/1‑211 (MQ=255) cTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtgt > 2:1078758/1‑234 (MQ=39) gTCCCCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACgg < 1:139766/236‑1 (MQ=37) cACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCg < 1:550256/212‑1 (MQ=38) aCCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 1:926485/236‑1 (MQ=38) ccgccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 2:779786/235‑1 (MQ=38) gccgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTc < 2:1075079/206‑1 (MQ=37) gccgACCAGACCTCGGTAGCCGAGAACGTCAAGCCGACCTTCACCGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCg < 2:427574/160‑1 (MQ=16) cgACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 1:897460/231‑1 (MQ=35) cgACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCAc < 2:530200/201‑1 (MQ=17) aGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGAGGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGCCCGCtggg > 1:372372/1‑204 (MQ=25) ccGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACt < 1:646556/235‑1 (MQ=25) cGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCt < 2:835590/137‑1 (MQ=34) ggTGGCTGAGTACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAGGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCTGCGCGCCATATACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 1:698540/235‑1 (MQ=16) ggTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 1:1078759/235‑1 (MQ=37) ggTGGCCGAGAACGTCAAACCGACCTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCGGGCCAGATCAGCCTGGGCATCGCGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 1:490021/235‑1 (MQ=255) gaACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGGCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTg < 1:184612/226‑1 (MQ=21) aaCGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCgg < 1:61109/235‑1 (MQ=33) aCGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGGGGATGTAGACGGTCGCACCTTCGAAAACCTGCaa > 2:292252/1‑221 (MQ=21) aCGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAACGCGATGACGTCTACAaggacg > 1:362349/1‑153 (MQ=25) tCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGcc < 2:220843/156‑1 (MQ=35) gcAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAGCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACAccc < 1:817505/116‑1 (MQ=25) caGCCGACCGTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGGGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGAGGCTGGCCAGATCAGCCTGGGCATCACGTCCg < 2:23842/177‑1 (MQ=255) aGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCa > 2:675794/1‑99 (MQ=18) gCCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCtg < 2:793788/179‑1 (MQ=19) ccGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:478963/142‑1 (MQ=34) cGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGCCCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCg < 2:984105/174‑1 (MQ=18) cGACCTACACCGGGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 2:136222/199‑1 (MQ=18) ttCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGGTGCGCAAGGCGATGACGTCTAGAACGACGCTGACCAGATAAGCCTGGGCATCa < 1:816646/163‑1 (MQ=255) ttCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGtt > 2:228258/1‑228 (MQ=32) cACCGTCCACGTCAACCAGCCGCTGGCGCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTa < 2:891602/129‑1 (MQ=25) ggCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCa < 2:319319/138‑1 (MQ=21) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACAcc < 1:692216/95‑1 (MQ=18) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAGACCAGCGCGCCATACAcc > 2:692215/1‑95 (MQ=18) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 2:320293/158‑1 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCaa > 2:734600/1‑221 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCaa < 1:734601/221‑1 (MQ=33) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATg > 2:664358/1‑163 (MQ=33) aaCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGACACTGTGCGGTGCGGCt > 1:43877/1‑207 (MQ=17) aCCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCAt < 2:1039283/145‑1 (MQ=34) tGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGc > 1:936355/1‑235 (MQ=33) gCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGCCGGTCGCACGTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAagtagt > 2:715982/1‑230 (MQ=18) gCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACCTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATac < 2:1079073/215‑1 (MQ=17) ccACGAACTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACATCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGAc < 1:469776/207‑1 (MQ=17) caCCGGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCGCGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 2:260963/160‑1 (MQ=18) acgtGGTCGTGGCCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATc < 1:990400/113‑1 (MQ=12) cTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGCCCGCTGTGGATGTAGACGGTCGCACCTTCGACAACCt > 2:277761/1‑167 (MQ=18) tGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACCCCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAagtagt < 1:57652/220‑1 (MQ=17) gggTCGTGACCCTGAGCAACAACGCCCAGGTCACCAGCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACt < 2:386988/171‑1 (MQ=18) gTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACcg < 2:1089453/232‑1 (MQ=32) tCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:880428/96‑1 (MQ=18) gTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCg > 2:476531/1‑184 (MQ=33) cccTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGTATCAAGTCGGCGGTGGATGTAGACGGTGGTACGTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcg < 1:545972/201‑1 (MQ=11) ccTGAGCAACAACGCCCAGGTCACCATCACGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGtc > 2:82120/1‑132 (MQ=21) cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTTTACAacg > 2:255292/1‑88 (MQ=18) cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAacg < 2:1045639/88‑1 (MQ=35) cTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGATGCGCAAGGCGATGACGTCTACAacg > 1:1045640/1‑88 (MQ=18) caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc < 2:162867/115‑1 (MQ=33) caacaaCGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTcc > 1:162867/1‑115 (MQ=33) aacaacGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCg < 1:1030070/235‑1 (MQ=32) caacGCTCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGATGACGTCTACAACGATGCGGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACcgacga < 1:58260/195‑1 (MQ=11) cacGCCCCGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGAc < 2:154868/178‑1 (MQ=17) aacGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGc < 1:914589/161‑1 (MQ=33) cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGc > 1:220690/1‑159 (MQ=32) cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAgg < 1:461157/234‑1 (MQ=31) cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCCGCTGTGGATGGCGACGGCCGCACCTTCGAGAACCTGCAACTGGGCGGTGCGGCTACCGTTCa > 2:243046/1‑171 (MQ=255) cGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCCGCTGTAGATGtcaacg > 2:545971/1‑120 (MQ=255) cccAGGTCCCCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGc < 1:192023/236‑1 (MQ=14) aGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCAAGTCCGCTGTAGATGTAGACGGTGGTACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTgg > 2:1018141/1‑232 (MQ=9) cACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGAc < 1:698441/207‑1 (MQ=31) aCCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGtaa > 1:83499/1‑215 (MQ=14) ccATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCAc < 1:765184/235‑1 (MQ=31) cATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCAc < 2:501824/234‑1 (MQ=31) aTCAAGGCCGGCGAAACCAGCGCGCCGTACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCtt < 2:365470/122‑1 (MQ=17) aTCAAGGACGGCGAAACCAGCGCGCCAGACACCCACGCTGCGCAAGGCGATTACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCg < 2:99788/124‑1 (MQ=12) cAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGc < 1:552139/46‑1 (MQ=38) aGGCCGGCGAGACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTaca < 1:168492/234‑1 (MQ=14) ggCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGaa < 2:268843/175‑1 (MQ=31) gctggCGATACCAGCGCGCCGTACACCCACGCTGCGCAGGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGAGGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGAc < 2:235804/188‑1 (MQ=9) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCgg < 1:449610/141‑1 (MQ=32) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCgg > 2:449609/1‑141 (MQ=32) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCCCCGaa > 2:510129/1‑212 (MQ=14) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCgag > 2:46834/1‑221 (MQ=31) ccGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATc < 1:228259/236‑1 (MQ=31) cGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATCAcc < 1:757898/235‑1 (MQ=11) | CAAGTTCGAACAGCTTACGCTGGACAAGACGCCGGTCAGCACGTCGGTGACCGACGAGCCAGGTACGCCGGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACCGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCCGGCGAAACCAGCGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCGCACCTTCGAAAACCTGCAACTGGGCGGTGCGGCTACCGTTCAAGTGACCGATACCACCGACGAAGTAGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATCACC > AE015451/215152‑215614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |