| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 258,519 | A→G | 5.6% | N18S (AAC→AGC) | PP_0207 → | putative Nitrate ABC transporter, periplasmic nitrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 258,519 | 0 | A | G | 5.6% | 243.2 / 4.3 | 72 | N18S (AAC→AGC) | PP_0207 | putative Nitrate ABC transporter, periplasmic nitrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (31/36); new base G (2/2); total (33/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-02 | |||||||||||
GCCATGCGAGAAGGACTGCCCCACCGGTGCGGTGAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCGGCAAGCTCGACTTCGGCGCCATGGCCGA > AE015451/258284‑258747 | gccatgcGAGAAGGACTGCCCCACCGGTGCGGTGAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCGCACTGGCC‑‑TGaa > 1:187462/1‑236 (MQ=255) cACCGGTGCGGTGAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGACACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGc > 1:666923/1‑218 (MQ=255) cACCGGTGCGGTGAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCa > 2:430123/1‑232 (MQ=255) cACCGGTGCGGTGAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATAc > 1:781511/1‑235 (MQ=255) gtgcggtgAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCg > 1:293975/1‑236 (MQ=255) gtgAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAgcg < 1:1046827/213‑1 (MQ=255) tgAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCa < 2:1046389/233‑1 (MQ=255) aGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCa > 1:744072/1‑236 (MQ=255) ggTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGc < 2:716697/234‑1 (MQ=255) tCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCTCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCAt < 1:836378/228‑1 (MQ=255) gACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCg < 1:1028666/228‑1 (MQ=255) gACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCACCGAAACCATACGCATCGCCATCGGCACCCAg > 1:671189/1‑236 (MQ=255) aCATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCg < 2:129201/206‑1 (MQ=255) cATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg < 1:1125275/235‑1 (MQ=255) cATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg < 1:394058/235‑1 (MQ=255) ttCCGTACCTGCTGCCCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg < 2:698299/233‑1 (MQ=255) ccTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCAt > 1:116567/1‑236 (MQ=255) cgcTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGcc > 1:361646/1‑188 (MQ=255) gCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCct < 1:430124/236‑1 (MQ=255) aTCCCGCGCACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg < 2:781924/202‑1 (MQ=255) gCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGcgcagttgatg < 1:160058/236‑11 (MQ=255) cGAACCCCCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCa < 2:893224/184‑1 (MQ=255) aCGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGc < 2:666922/171‑1 (MQ=255) aCGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCCGCCTCGCGCTGGCGCCCACTGGCC‑‑TGAACGCCa > 2:794099/1‑149 (MQ=255) gCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGcc < 2:867598/234‑1 (MQ=255) cctcctGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCctgct < 1:967857/236‑1 (MQ=255) cctGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTgg > 1:823789/1‑235 (MQ=255) gctgTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCAt > 2:721755/1‑152 (MQ=255) gctgTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGc < 1:563483/225‑1 (MQ=255) gtgCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAgc < 2:587476/138‑1 (MQ=255) gCCCCTCGTTCCCCCCCACCCAAGCCGGGCGGCTGAGACGAAGCCCACTCTTATAACGACTCGAGGGGAGACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTg < 2:193101/186‑1 (MQ=255) cccTCGTTTCCCACCACCCAAGCCGGGTGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCctgc > 1:1069086/1‑201 (MQ=255) cccTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCct < 2:1029015/220‑1 (MQ=255) gTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGTTCA‑‑GGCCAGTGAGCGCCAGCGCCGAAACCATACGCATCGCCa < 1:808970/151‑1 (MQ=255) gTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGTTCA‑‑GGCCAGTGAGCGCCAGCGCCGAAACCATACGCATCGCCa > 2:808969/1‑151 (MQ=255) gTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCctgct > 2:711275/1‑197 (MQ=255) caccCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCct < 1:792121/185‑1 (MQ=255) cGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGa > 1:81002/1‑236 (MQ=255) gggCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGAc < 1:31634/236‑1 (MQ=255) cggAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg > 2:75810/1‑138 (MQ=255) cggAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg < 1:75810/138‑1 (MQ=255) ggAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCacgac < 1:550686/214‑1 (MQ=255) ggAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCAcc < 1:788011/235‑1 (MQ=255) agaCGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCac > 2:1014044/1‑209 (MQ=255) aCGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTAc > 1:762489/1‑234 (MQ=255) aCGAAGCCAACTCTTATAACGAGTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTAcc < 1:190516/235‑1 (MQ=255) ccAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCAGACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCct < 2:103834/158‑1 (MQ=255) aaCGATTCGAGGGGAAACACCCAGGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGc < 2:114835/109‑1 (MQ=255) cGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAg > 1:889475/1‑192 (MQ=255) cGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGCGTGGAAGAACTTCACCa > 1:108691/1‑235 (MQ=255) aGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAg > 1:763364/1‑217 (MQ=255) aaaCACCCATGCGCCTTGTTGCAACCGTCGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGACCGCCAGCGCCGAAACCATACGCAt < 2:333327/85‑1 (MQ=255) cacCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGcgcc < 1:949000/235‑1 (MQ=255) ccATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCATGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGc < 2:1029182/168‑1 (MQ=255) gcCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGc > 2:1082316/1‑119 (MQ=255) gcCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGc < 1:1082317/119‑1 (MQ=255) cTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGcc > 1:468504/1‑139 (MQ=255) tgttgCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCCCCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGt < 2:116567/197‑1 (MQ=255) tgCAACCGTGGCCGGCCTCGCGCTGTCGCTCACTCGCC‑‑TGAACGcc > 2:1103129/1‑46 (MQ=255) tgCAACCGTGGCCGGCCTCGCGCTGGCGTTCA‑‑GGCCAGTGAGCGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGc < 1:548462/128‑1 (MQ=38) tgCAACCGTGGCCGGCCTCGCGCTGGCGTTCA‑‑GGCCAGTGAGCGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGc > 2:548461/1‑128 (MQ=38) cAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTc > 1:968043/1‑236 (MQ=255) gTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCCCCCCGGACACCACCATCAACTGCGCCACCGGTGGCCCGCTGAGCCgcg > 2:46721/1‑116 (MQ=255) ccggccTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGgaag < 2:688860/188‑1 (MQ=255) ccggccTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGgaag > 1:688861/1‑188 (MQ=255) ccggccTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCGGCaac > 1:653795/1‑235 (MQ=255) ccggccGCGCGCGGGCGCTGACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCACCACCGGTGGCCTGCGGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTAcc < 2:97355/174‑1 (MQ=255) ggccTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCg > 1:874809/1‑229 (MQ=255) cgcgCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAgg > 2:1118946/1‑155 (MQ=255) gcTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTg > 1:1102948/1‑168 (MQ=255) tGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCGGCAAGCTCGACTTCGGCGCCATg < 2:968042/232‑1 (MQ=255) tGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCGGCAAGCTCGACTTCGGCGCCATGGcc < 1:711276/235‑1 (MQ=255) ggCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCGGCAAGCTCGACTTCGGCGCCATGGCCGa > 1:763173/1‑236 (MQ=255) | GCCATGCGAGAAGGACTGCCCCACCGGTGCGGTGAAGGTCGACATTCCGTACCTGCTGCGCTGATTCATCCCGCCATCCGGCGAACCACCGGACGCCCTCCTGCTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCC‑‑TGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACCAGCGGTGCGCCGCTGACCAACGAAATGGTCGCCGGCAAGCTCGACTTCGGCGCCATGGCCGA > AE015451/258284‑258747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |