Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 1,070,246:1 +GA 100% intergenic (+46/+96) aroP‑I → / ← PP_0928 aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,070,2461.G97.5% 292.6 / 0.2 80intergenic (+46/+96)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (36/42);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*AE0154511,070,2462.A97.5% 297.1 / ‑0.3 80intergenic (+46/+96)aroP‑I/PP_0928aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger related‑protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (36/41);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATG  >  AE015451/1070037‑1070456
                                                                                                                                                                                                                    ||                                                                                                                                                                                                                  
ggCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCgcggg                                                                                                                                                                                                  <  2:1095974/230‑1 (MQ=255)
ggCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑Gagatc                                                                                                                                                                                                              >  2:1011179/1‑212 (MQ=255)
       gCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtct                                                                                                                                                                                     >  1:789860/1‑236 (MQ=255)
             cAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCcgcgcg                                                                                                                                                                                                    <  2:920596/215‑1 (MQ=255)
                 tACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGa                                                                                                                                                                            <  1:669335/235‑1 (MQ=255)
                    cTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGa                                                                                                                                                                            <  2:688219/232‑1 (MQ=255)
                                           gATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGCGCGCCGCgcgggcg                                                                                                                                                                                                <  2:1012280/189‑1 (MQ=255)
                                                   tAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAc                                                                                                                                         <  1:1053847/236‑1 (MQ=255)
                                                    aGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTGACAGGCGATGaaa                                                                                                                                                                       >  2:583526/1‑205 (MQ=255)
                                                    aGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATg                                                                                                                                                                          >  2:1129785/1‑202 (MQ=255)
                                                          gTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTg                                                                                                                                           <  1:93491/227‑1 (MQ=255)
                                                           tACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTc                                                                                                                                   >  2:1101533/1‑234 (MQ=255)
                                                                gACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCcgcgcg                                                                                                                                                                                                    >  2:709108/1‑164 (MQ=255)
                                                                     cgccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCt                                                                                                                                                                                           >  2:13398/1‑168 (MQ=255)
                                                                          aTTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTCAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATgc                                                                                                                                                                         >  2:234943/1‑180 (MQ=255)
                                                                          aTTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCGGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCt                                                                                                                                                     >  2:978457/1‑201 (MQ=255)
                                                                                   tcGGTAATGCTGATCCCGGCATGGATCGCTGTTCTGTGGGGGGCTTTCAAGCTGAAGAAGGGTCGCCAGGCCAAGGGGTGATCGCGTGTGTTCGTAAGGGGTATGCCGTTAGGTGTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCgcgggcgg                                                                                                                                                                                               <  1:1210620/150‑1 (MQ=255)
                                                                                    cGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc                                                                                                        <  1:496109/236‑1 (MQ=255)
                                                                                          tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGAGCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCaa                                                                                                  <  1:318497/236‑1 (MQ=255)
                                                                                             tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCCTGCCCCTCAATGCTGCCTCCGCCAAg                                                                                                 >  1:433776/1‑234 (MQ=255)
                                                                                             tGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGccccgcc                                                                                                                  >  1:290423/1‑214 (MQ=255)
                                                                                              gATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                              <  1:391595/236‑1 (MQ=255)
                                                                                               aTCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGAGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGcc                                                                                                                       >  1:1209034/1‑210 (MQ=255)
                                                                                                tCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgc                                                                                            <  1:440765/236‑1 (MQ=255)
                                                                                                  ccGGCAGGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa                                                                                                   <  1:6586/227‑1 (MQ=255)
                                                                                                    ggCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCGGCCTCCGCCCAGCACGcaca                                                                                         >  1:288749/1‑235 (MQ=255)
                                                                                                    ggCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCGCGa                                                                                                                                                                                        >  1:411210/1‑140 (MQ=255)
                                                                                                      cATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                      <  1:1011184/236‑1 (MQ=255)
                                                                                                       aTGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                      >  1:1200408/1‑235 (MQ=255)
                                                                                                                     tgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAacac                                                                        >  1:837818/1‑235 (MQ=255)
                                                                                                                     tgtgGGTGGCTTTCAAGCTGAAGAAGACTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGc                                                                                                                                                         >  2:854619/1‑154 (MQ=255)
                                                                                                                      gtgggtggCTTTCAAGCTGAAGAAGGCTCGCCGGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                      <  1:51258/220‑1 (MQ=255)
                                                                                                                               tttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCCGGCGGCGTCCGATCTCACAGGCGAt                                                                                                                                                                           >  2:34541/1‑126 (MQ=38)
                                                                                                                                ttCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAc                                                            <  1:655605/236‑1 (MQ=255)
                                                                                                                                    aGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                          >  2:950618/1‑234 (MQ=255)
                                                                                                                                     gcggaagaaGGCTCGCCGGGCCAAGGGCTGTTCGGGTTTGTGCGTAAGGCGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTg                                                                                                                                           <  1:759141/149‑1 (MQ=37)
                                                                                                                                            aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                              <  1:947901/190‑1 (MQ=255)
                                                                                                                                            aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGcaca                                                                                         >  1:98869/1‑195 (MQ=255)
                                                                                                                                            aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                      <  1:943490/198‑1 (MQ=255)
                                                                                                                                              ggCTCGCCAGGCCAAGGGCTGGTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATa                                                <  2:574624/234‑1 (MQ=255)
                                                                                                                                                cTCGCCAGGCCAAGGGCTGATCGCGTTTGTTCGTGAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCg                                                                 <  2:1191914/215‑1 (MQ=255)
                                                                                                                                                   gCCAGGCCAAGGGCTGATCGGGTTTGTGCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTcc                                                                                                       <  2:498066/174‑1 (MQ=255)
                                                                                                                                                    ccAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                          <  1:634175/218‑1 (MQ=255)
                                                                                                                                                    ccAGGCCAAGGGCTGAGCGGGGTTGTTCGGAAGGGGTATGCCGTTAGGTTTTCGGCGCCGGTGA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgc                                                                                                <  2:311124/180‑1 (MQ=39)
                                                                                                                                                       ggCCAAGGGCTGATCCGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                              <  2:1058661/179‑1 (MQ=255)
                                                                                                                                                         caaaGGGCTGATCGGGTTTGTTCTTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCa                                                                            <  2:774548/193‑1 (MQ=255)
                                                                                                                                                                cTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCa                            <  1:224590/236‑1 (MQ=255)
                                                                                                                                                                   aTCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa                                                                                                   <  2:447703/162‑1 (MQ=255)
                                                                                                                                                                    tctggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAggc                         <  1:611665/232‑1 (MQ=255)
                                                                                                                                                                     ggggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGc                                                                                                                        <  2:185543/138‑1 (MQ=255)
                                                                                                                                                                     cGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAggcg                        >  1:712688/1‑235 (MQ=255)
                                                                                                                                                                      gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                                                                                                                  <  2:1181911/80‑1 (MQ=25)
                                                                                                                                                                      gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                                                                                                                  >  1:1181916/1‑80 (MQ=21)
                                                                                                                                                                      gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCCATGCTGCCTCCGCCAAgcacgcac                                                                                          >  2:1150317/1‑168 (MQ=255)
                                                                                                                                                                      gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGaa                                             >  1:498623/1‑213 (MQ=255)
                                                                                                                                                                      gggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAgtcggc                      >  1:318884/1‑236 (MQ=255)
                                                                                                                                                                        gTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                          <  2:778484/198‑1 (MQ=255)
                                                                                                                                                                         tttGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGgcc                     <  1:1234016/234‑1 (MQ=255)
                                                                                                                                                                           tGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAg                                                                              >  1:363233/1‑175 (MQ=255)
                                                                                                                                                                                gTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                              <  1:963707/154‑1 (MQ=255)
                                                                                                                                                                                gTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                              >  2:963702/1‑154 (MQ=255)
                                                                                                                                                                                 tAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGc           <  1:1129790/236‑1 (MQ=255)
                                                                                                                                                                                    ggggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAc                                                            <  2:911450/184‑1 (MQ=255)
                                                                                                                                                                                     gggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGc                                                                                                     >  2:277419/1‑142 (MQ=255)
                                                                                                                                                                                     gggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgc                                                                                                >  2:961949/1‑147 (MQ=255)
                                                                                                                                                                                     gggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                      >  2:284210/1‑157 (MQ=255)
                                                                                                                                                                                     gggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg                                                                                      <  1:284214/157‑1 (MQ=255)
                                                                                                                                                                                     gggTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATAc       >  1:260297/1‑236 (MQ=255)
                                                                                                                                                                                          tGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATg  >  1:293856/1‑236 (MQ=255)
                                                                                                                                                                                           gCCGTGAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGc                                                        <  1:277842/181‑1 (MQ=255)
                                                                                                                                                                                            ccGTTAGGTGTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCCTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCg                                                          <  1:1013808/178‑1 (MQ=255)
                                                                                                                                                                                             cGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCtt                                                                                                                              <  1:1243343/109‑1 (MQ=255)
                                                                                                                                                                                             cGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCtt                                                                                                                              >  2:1243338/1‑109 (MQ=255)
                                                                                                                                                                                             cGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggcg                                                                                   >  2:340136/1‑150 (MQ=255)
                                                                                                                                                                                             cGTTAGCTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCAATCGCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGt                                                                                                                                     >  2:973523/1‑102 (MQ=25)
                                                                                                                                                                                              gTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacgca                                                                                           <  2:363228/143‑1 (MQ=255)
                                                                                                                                                                                               ttAGGTTTTCGGCGCC‑‑TGTGCGATCGAGCGCCGCGCGGGCGGCGCTCGCTCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCa                                                                                                   <  2:855602/134‑1 (MQ=255)
                                                                                                                                                                                                   gTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcac                                                                                              >  2:887687/1‑135 (MQ=255)
                                                                                                                                                                                                   gTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCGAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacg                                                                                             >  2:448104/1‑136 (MQ=255)
                                                                                                                                                                                                          gcgcC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCAcc                                                           <  2:281401/163‑1 (MQ=255)
                                                                                                                                                                                                                    ||                                                                                                                                                                                                                  
GGCACCCGCTGACCAACTACCTGTGCCTGGCGTTCATCGTGCTGATCCTGGTAGTGATGTACCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAACACCGTAGCGAGCACCGGCAGTACATAGAACAGCATCAATCGCTCCAGGCGGCCAGCCAACGGCATACCCATG  >  AE015451/1070037‑1070456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑