Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	5,201,568	Δ98 bp	39.5%	intergenic (‑54/‑29)	PP_4582 ← / → PP_4583	conserved membrane protein of unknown function/putative Peptidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5201567	40 (0.560)	20 (0.290)	17/344	NT	39.5%	intergenic (‑53/‑127)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase
	?	AE015451	5201666 =	22 (0.310)					intergenic (‑152/‑28)	PP_4582/PP_4583	conserved membrane protein of unknown function/putative Peptidase

CGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCC  >  AE015451/5201334‑5201776                                                                                                                                                                                                                                          |                                                                                                                                                                                                                  cGAAGCTGTCCTTGACCGGGTCGGCGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                 <  1:1210826/236‑1 (MQ=255) cGAAGCTGGCCTTGACCGGGTCGACGCTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                 <  1:1032075/236‑1 (MQ=255)      cTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAG‑TGCCTATGATAAACACTTTTCCGGGCacaggca                                                                                                                                                                                                            <  1:588646‑M1/235‑8 (MQ=255)            ttGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGtt                                                                                                                                                                                                       >  1:387863/1‑235 (MQ=255)            ctGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACCCTTTTCCGGgcgc                                                                                                                                                                                                                 <  1:1106890/224‑1 (MQ=255)                         cgaTGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacagg                                                                                                                                                                                                              <  1:1063901‑M1/215‑6 (MQ=255)                                 ggggTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTcgcg                                                                                                                                                                                 >  1:957803/1‑236 (MQ=255)                                  gggTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTcgcg                                                                                                                                                                                 <  1:942125/235‑1 (MQ=255)                                  gggTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCgg                                                                                                                                                                                <  1:864043/236‑1 (MQ=255)                                       agcagcagcACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaa                                                                                                                                                                            <  1:1143679/235‑1 (MQ=255)                                           gcagcaCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTc                                                                                                                                                                                    >  1:659106/1‑223 (MQ=255)                                               caCCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGtt                                                                                                                                                                                                       <  1:176765/200‑1 (MQ=255)                                                cccgCCAGCCCCCCGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCCCCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatca                                                                                                                                                                                                  <  1:416232‑M1/203‑18 (MQ=255)                                                  cGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagaga                                                                                                                                                                                             >  2:851441‑M1/1‑185 (MQ=255)                                                    ccAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGGGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCgg                                                                                                                                                                                >  1:261944/1‑218 (MQ=255)                                                      aGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCg                                                                                                                                                            <  1:303152/236‑1 (MQ=255)                                                         accacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGgcgc                                                                                                                                                                                                                 >  1:72519/1‑180 (MQ=255)                                                         accacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGt                                                                                                                                                          <  1:667235/235‑1 (MQ=255)                                                         accacGCGTAGCGGTGCGCTCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCaca                                                                                                                                                                                                                <  1:115878‑M1/181‑4 (MQ=255)                                                         accacGCGTAGCGGTACGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCTGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATGAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGt                                                                                                                                                          <  1:1209160/235‑1 (MQ=255)                                                           cacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGc                                                                                                                                                        <  1:463686/235‑1 (MQ=255)                                                           cacGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGACTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGc                                                                                                                                                        <  1:713128/235‑1 (MQ=255)                                                            acGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGc                                                                                                                                                        <  1:119518/234‑1 (MQ=255)                                                               cgTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATcc                                                                                                                                                   >  1:158976/1‑236 (MQ=255)                                                                     ggTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTg                                                                                                                                             <  1:1085434/236‑1 (MQ=255)                                                                      gTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccagg                                                                                                                                            <  1:851049‑M1/236‑72 (MQ=255)                                                                       ttCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCctc                                                                                                                                                                                       <  2:387858/192‑1 (MQ=255)                                                                             cagccagcGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGcc                                                                                                                                                                                             <  2:506763/180‑1 (MQ=255)                                                                                ccagcGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTg                                                                                                                                             <  2:957798/225‑1 (MQ=255)                                                                                 cagcGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGGTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCGCCTACCTCGGTGCGATCCCt                                                                                                                                                 >  1:210483/1‑220 (MQ=255)                                                                                  agcGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaa                                                                                                                                                                                                 <  2:396739‑M1/171‑19 (MQ=255)                                                                                    cgcCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggcca                                                                                                                                         <  1:544056‑M1/225‑75 (MQ=255)                                                                                      ccACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaa                                                                                                                                                                            <  2:315352/188‑1 (MQ=255)                                                                                             cAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTCGTCGTACAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagacaccttcaatgcc                                                                                                                                                                                  >  2:1167404‑M1/1‑142 (MQ=255)                                                                                                      aGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTCTTCCGGGCGCAGACAGTGTTTGCCTCTGc                                                                                                                                                                                              >  2:93437/1‑154 (MQ=255)                                                                                                         ttGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCcgc                                                                                                                             <  1:373690/216‑1 (MQ=255)                                                                                                         ttGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGa                                                                                                                           >  1:634233/1‑218 (MQ=255)                                                                                                             aGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTcc                                                                                                     >  1:1193811/1‑236 (MQ=255)                                                                                                              gCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccc                                                                                                    >  1:592729‑M1/1‑125 (MQ=255)                                                                                                                                 aaGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatg                                                                                                                             <  1:605985‑M1/192‑87 (MQ=255)                                                                                                                                  aGGCGGCTACGGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGAGGCCTATGATAACCAC‑TTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTaa                                                                                                                                                                            <  2:296041/143‑1 (MQ=255)                                                                                                                                    gcggcTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATTGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGtt                                                                                                                                    <  1:5856/182‑1 (MQ=255)                                                                                                                                     cggcTTCTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGCTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACTCTTTTCCGGGCacaggca                                                                                                                                                                                                            <  2:398228‑M1/109‑8 (MQ=255)                                                                                                                                         tACTGCGTCCAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggcc                                                                                                                                          <  1:196599‑M1/171‑74 (MQ=255)                                                                                                                                           cTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCCGCCTCTTCGCGGGTAAACCAGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGt                                                                                                                                     >  2:229470/1‑174 (MQ=255)                                                                                                                                              cGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGtt                                                                                                                                              <  2:1266205/162‑1 (MQ=255)                                                                                                                                               gTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGcccc                                                                     >  1:1256876/1‑234 (MQ=255)                                                                                                                                               gTACAAGCCCCAGGCCAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTAAGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacctccgattgcccaagccgaccaggccaccgacccgtatg                                                                                                                             <  2:717023‑M1/178‑87 (MQ=255)                                                                                                                                                tACAAGCCCCAGGCGAAGGTGTGGCCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACCCTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccacccaagccccaacccc                                                                                                                                                                 <  2:217703‑M1/141‑51 (MQ=255)                                                                                                                                                 aCAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGccaac                                                                             >  1:527151/1‑224 (MQ=255)                                                                                                                                                   aaGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAAcccc                                                               >  1:764523/1‑236 (MQ=255)                                                                                                                                                   aaGCCCCAGGCCAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgccc                                                                                                                                                        <  1:9369‑M1/147‑60 (MQ=255)                                                                                                                                                                  gtgtAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCtt                                                                                       >  1:1112674/1‑197 (MQ=255)                                                                                                                                                                    gtAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgatcgcccaagccgaccagg                                                                                                                                            >  2:483723‑M1/1‑71 (MQ=255)                                                                                                                                                                                ggTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGcaa                                                                                                               >  2:1026513/1‑158 (MQ=255)                                                                                                                                                                                  tCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCACTCAAGAGGCCTTc                                                                                      >  2:242066/1‑182 (MQ=255)                                                                                                                                                                                             gtggacgccaggcAACGAGATGCCTATGATAAACACGTATCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggcca                                                                                                                                         <  1:784972‑M1/114‑75 (MQ=255)                                                                                                                                                                                             gTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgct                                                                                       >  2:192135‑M1/1‑46 (MQ=255)                                                                                                                                                                                              tGCAAGCCAGGCAAAGAGAGGCCTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcc                    <  1:374320‑M1/236‑192 (MQ=255)                                                                                                                                                                                               gCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGACCCAAc                                                                  >  1:324993/1‑189 (MQ=255)                                                                                                                                                                                                       tgCAAAGAGCTGCCTATGATAAACACTTTTCCGTGCGCAGACAGTTTTTGCCTGGGCCGGCCTCTTCGCGGGTCAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGtt                                                                                                                                    <  1:68174/114‑1 (MQ=38)                                                                                                                                                                                                                    cTATGATAAACACTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcctgttc               >  2:775890‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                     tATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAAca                                                                 >  2:699550/1‑167 (MQ=255)                                                                                                                                                                                                                               acTTTTCCGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcac                                   <  2:634228‑M1/188‑177 (MQ=255)                                                                                                                                                                                                                                cTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCg                                                             >  2:894354/1‑161 (MQ=255)                                                                                                                                                                                                                                    ggcggGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccacc                                                                                                                                       <  1:892097‑M1/81‑77 (MQ=255)                                                                                                                                                                                                                                     ccGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGa                                                                                                                                           >  2:655195/1‑78 (MQ=255)                                                                                                                                                                                                                                      cGGGCacaggcaactccaatcaagagcccttcaatgccaaccaagccccaacccccgattgcccaagccgcccaggccaccgacccgtatgtctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcctgttcgaagaaatcaagg  <  1:195091‑M1/214‑210 (MQ=255)                                                                                                                                                                                                                                      cGGGCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcg                                                                                                             <  2:329683‑M1/107‑103 (MQ=255)                                                                                                                                                                                                                                       gggCacaggcaactccaatcaagagaccttcaatgccaaccacgccccaacccccgatggcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcctgtt                <  2:1112669‑M1/199‑196 (MQ=255)                                                                                                                                                                                                                                       gggCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggcca                                                                                                                                         <  1:655200‑M1/78‑75 (MQ=255)                                                                                                                                                                                                                                        ggCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggcca                                                                                                                                         <  2:1074669‑M1/77‑75 (MQ=255)                                                                                                                                                                                                                                         gcgcAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCaa                >  1:977667/1‑197 (MQ=255)                                                                                                                                                                                                                                         gCacaggcaactccaatcaagagaccttcaatgccaaccaagccccaacccccgattgcccaagccgaccaggccaccgacccgtatgcctggctgcaacagcgcgacacccccgaggtactcgcttacctgcaagctgaaaacgcctaccaggaagcctgcctggccgaccaggcaccgttgcgtgagcgcctgttcgaagaaatcaagg  <  2:977662‑M1/211‑210 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                  CGAAGCTGTCCTTGACCGGGTCGACGATGGTCGGGGTCAGCAGCAGCACCGCCAGCACCACGCGTAGCGGTTCGCGCAGCCAGCGCCACATCCAGCCGGTGAGCTTGAAGCCCACCAGCAGGCACCCTAAGGCGGCTACTGCGTACAAGCCCCAGGCGAAGGTGTAGTCGTTCTCGGTCATGGTGTTCGTGCAAGCCAGGCAAAGAGATGCCTATGATAAACACTTTTCCGGGCGCAGACAGTGTTTGCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACCTCGGTGCGATCCCTGTTGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACTCCAATCAAGAGACCTTCAATGCCAACCAAGCCCCAACCCCCGATTGCCCAAGCCGACCAGGCCACCGACCCGTATGCCTGGCTGCAACAGCGCGACACCCC  >  AE015451/5201334‑5201776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.