Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 1,184,561 A→T 5.3% intergenic (‑177/‑235) mltF ← / → purL membrane‑bound lytic murein transglycosylase F/Phosphoribosylformylglycinamidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154511,184,5610AT 5.3% 279.6 / 5.7 75intergenic (‑177/‑235)mltF/purLmembrane‑bound lytic murein transglycosylase F/Phosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base A (37/34);  new base T (2/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

GAAGCCATTTGGCGCAACGCTGGCGCAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCC  >  AE015451/1184339‑1184791
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      
gaAGCCATTTGGCGCAACGCTGGCGCAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGTGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCG‑CAGGTGTCCGGCTCGACACATTCTGCGCCTGTGAGATc                                                                                                                                                                                                                          >  1:1132062/1‑236 (MQ=255)
                  gCTGGCGCAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCgcgggcg                                                                                                                                                                                                          >  1:1025642/1‑235 (MQ=255)
                   cTGGCGCAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCgcggg                                                                                                                                                                                                            <  2:777634/232‑1 (MQ=255)
                      gcgcAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGTGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCG‑CAGGTGTCCGGCTCGACACATTCTGCGCCTGTGAGATCGAGCGCCGCCCGCGCGgcgc                                                                                                                                                                                                      <  2:1132057/234‑1 (MQ=37)
                       cgcAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGgcggcg                                                                                                                                                                                                       <  2:916377/233‑1 (MQ=255)
                         cAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGAGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCt                                                                                                                                                                                                                                  <  2:723236/204‑1 (MQ=255)
                               aGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCgcgggcgg                                                                                                                                                                                                         >  2:940834/1‑223 (MQ=255)
                                 tgtgGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                                                                                           <  1:259870/235‑1 (MQ=255)
                                   tgGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGgcgc                                                                                                                                                                                                      >  1:308742/1‑222 (MQ=255)
                                   tgGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgc                                                                                                                                                                                        >  1:151086/1‑236 (MQ=255)
                                              aGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAAtgtg                                                                                                                                                                              >  1:115467/1‑235 (MQ=255)
                                                  tGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGa                                                                                                                                                                          >  1:1221210/1‑235 (MQ=255)
                                                    cAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGcgccg                                                                                                                                                                                                                  <  2:859899/193‑1 (MQ=255)
                                                        atccGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGGGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGGCTCGACACATTCTGCGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCATAGgcgct                                                                                                                                                                                     <  1:330722/215‑2 (MQ=25)
                                                           cGCAAAGGACTGGCCCAACCATATCTCGGCAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTc                                                                                                                                                                            <  2:1054372/224‑1 (MQ=255)
                                                           cGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtctc                                                                                                                                                                                              <  1:611990/206‑1 (MQ=255)
                                                            gCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGACTGTGTCGAGCCGGacac                                                                                                                                                                 >  2:681810/1‑234 (MQ=255)
                                                                 ggACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAAtgtg                                                                                                                                                                              >  1:812781/1‑216 (MQ=255)
                                                                 ggACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCAGCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGgcgccc                                                                                                                                                                                                    >  1:907387/1‑192 (MQ=255)
                                                                    cTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTc                                                                                                                                                                            >  1:8456/1‑215 (MQ=255)
                                                                      ggCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtctc                                                                                                                                                                                              >  2:867382/1‑195 (MQ=255)
                                                                       gCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCg                                                                                                                                                     <  1:867387/235‑1 (MQ=255)
                                                                           aaCCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGAtctc                                                                                                                                                <  1:859309/236‑1 (MQ=255)
                                                                               atatCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATgtgt                                                                                                                                                                             >  2:512383/1‑203 (MQ=255)
                                                                               atatCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGac                                                                                                                                                                   >  1:981122/1‑213 (MQ=255)
                                                                                    tcGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGaca                                                                                                                                                                  >  1:525274/1‑209 (MQ=255)
                                                                                      gACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGcc                                                                                                                                      <  1:1057073/235‑1 (MQ=255)
                                                                                       aCAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAgg                                                                                                                                                                                         <  2:609371/183‑1 (MQ=255)
                                                                                       aCAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGcc                                                                                                                                      <  1:1208634/234‑1 (MQ=255)
                                                                                       aCAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCgg                                                                                                                                    <  1:472447/236‑1 (MQ=255)
                                                                                       aCAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGCCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGcc                                                                                                                                      <  2:724198/234‑1 (MQ=255)
                                                                                          aCGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGa                                                                                                                                                                          >  2:1286015/1‑195 (MQ=255)
                                                                                          aCGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGaca                                                                                                                                 >  1:446971/1‑236 (MQ=255)
                                                                                          aCGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGac                                                                                                                                  <  1:664419/235‑1 (MQ=255)
                                                                                           cGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGgcgcgc                                                                                                                                                                                                    >  2:114105/1‑166 (MQ=255)
                                                                                                          gTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGAcc                                                                                                                 >  1:29853/1‑236 (MQ=255)
                                                                                                                   tgtgtgGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTGGAGATATTCAGCTCCTGTTAGATGGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGGGCCGCgcgggcg                                                                                                                                                                                                          >  2:913212/1‑138 (MQ=255)
                                                                                                                               ggAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTgc                                                                                                                                                             >  2:1262407/1‑171 (MQ=255)
                                                                                                                                  ggggggATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAgcg                                                                                                                                                                                                                     >  1:950370/1‑112 (MQ=255)
                                                                                                                                       gATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTgcg                                                                                                                                                            >  2:967163/1‑164 (MQ=255)
                                                                                                                                           gCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTgc                                                                                                                                                             >  2:329800/1‑159 (MQ=255)
                                                                                                                                              tCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAg                                                                                      <  2:1221205/227‑1 (MQ=255)
                                                                                                                                                aGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTgc                                                                                                                                                             <  2:1243487/154‑1 (MQ=255)
                                                                                                                                                    cTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCgg                                                                                                                                    <  1:233898/175‑1 (MQ=255)
                                                                                                                                                       ccAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATGGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACAcc                                                                                                                                                                <  2:609763/144‑1 (MQ=255)
                                                                                                                                                         aGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCg                                                                                                                                                                                                                         >  2:802496/1‑85 (MQ=255)
                                                                                                                                                           gTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGaa                                                                                                            <  2:115464/192‑1 (MQ=255)
                                                                                                                                                           gTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGcc                                                                 <  1:1030366/235‑1 (MQ=255)
                                                                                                                                                                 ggCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGAtcc                                                                                                                                                 >  1:70061/1‑148 (MQ=255)
                                                                                                                                                                      gagaTATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATc                                                       <  1:719944/234‑1 (MQ=255)
                                                                                                                                                                         atatTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGc                                                                  <  1:27069/220‑1 (MQ=255)
                                                                                                                                                                           atTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAAtt                                                  <  2:446966/234‑1 (MQ=255)
                                                                                                                                                                             tCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTgcgg                                                                                                                                                           <  2:1105397/127‑1 (MQ=255)
                                                                                                                                                                                gcgcCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCg                                                                                   >  2:1028310/1‑196 (MQ=255)
                                                                                                                                                                                 cgcCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAgg                                               <  2:1025637/231‑1 (MQ=255)
                                                                                                                                                                                    cTGTGAGATCGAGCGCCGCCATGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCcgcgcg                                                                                                                                                                                                              >  2:19026/1‑69 (MQ=255)
                                                                                                                                                                                    cTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGa                                                               >  2:857327/1‑212 (MQ=255)
                                                                                                                                                                                    cTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATg                                       <  1:193759/236‑1 (MQ=255)
                                                                                                                                                                                       tgAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCAGCGCTCGATCTCGTCTCAAGCCgg                                                                                                                                    >  2:724837/1‑140 (MQ=255)
                                                                                                                                                                                         agaTCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACgg                                  >  1:668754/1‑236 (MQ=255)
                                                                                                                                                                                           cgcgcgcgcCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGAGCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGc                                                                        <  2:351033/191‑1 (MQ=255)
                                                                                                                                                                                           aTCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGa                                                               <  2:1055867/205‑1 (MQ=255)
                                                                                                                                                                                            tCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGcc                                <  1:1154119/235‑1 (MQ=255)
                                                                                                                                                                                                  gccgccAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGCAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCCTAGAGCa                                                                       >  2:201369/1‑190 (MQ=255)
                                                                                                                                                                                                   ccgccAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGCTCTCACAGGCGCAGAATGTGTCGAGCCCGa                                                                                                                                                                    >  2:91235/1‑96 (MQ=255)
                                                                                                                                                                                                              ccGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGc                                                                               <  2:1245725/170‑1 (MQ=255)
                                                                                                                                                                                                                gCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGCAAGCCATCGAATTAGGCTAGCATGCACGGCCTCTAAGcac                       >  1:369613/1‑224 (MQ=255)
                                                                                                                                                                                                                 ccTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGa                                                                                                             >  2:1273589/1‑137 (MQ=255)
                                                                                                                                                                                                                  cTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTcc              >  2:1243475/1‑231 (MQ=255)
                                                                                                                                                                                                                   ttAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGcc                                <  1:818499/212‑1 (MQ=255)
                                                                                                                                                                                                                   gtAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGTCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGc                                      <  1:1193723/205‑1 (MQ=255)
                                                                                                                                                                                                                     gacacatTCTGCGCCTGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAAt                                                   >  2:1234300/6‑191 (MQ=255)
                                                                                                                                                                                                                       gATAATCAGCGCCTATGAGATCGCGCGCCGCGCGGGCGGCGCTCGATCTCACACGCGCAGAATGTGTCgcgc                                                                                                                                                                        >  2:10327/1‑72 (MQ=21)
                                                                                                                                                                                                                        atatTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGt     >  2:978428/1‑234 (MQ=255)
                                                                                                                                                                                                                         tatTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTccc  <  1:1243480/236‑1 (MQ=255)
                                                                                                                                                                                                                              aGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCCTAGAGCAGGTGCCGAAAGCCATCGAAtt                                                  >  1:922315/1‑183 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                      
GAAGCCATTTGGCGCAACGCTGGCGCAAAGCAGTGTGGGCGAACATAGGTTGCAGTATACGCAAAGGACTGGCCCAACCATATCTCGACAACGGTTAGCTTGTCTGGTAGCGGGTTGTGTGGTTGGGGGAGGGGGGATTGCATCAGGGCTGCCAGGTGGCCGGCTTGAGATATTCAGCGCCTGTGAGATCGAGCGCCGCCAGGTGTCCGCCTTAAGATATTCAGCGCCTATGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCAGAATGTGTCGAGCCGGACACCTGCGGCGCTCGATCTCGTCTCAAGCCGGACACCTCTGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCC  >  AE015451/1184339‑1184791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑