BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	1,530,160	C→T	100%	R395C (CGT→TGT) noncoding (49/244 nt)	ftsZ → PP_mr19 →	Cell division protein FtsZ Xoo8 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	1,530,160	0	C	T	93.9%	341.9 / 8.3	99	R395C (CGT→TGT) noncoding (49/244 nt)	ftsZ PP_mr19	Cell division protein FtsZ Xoo8 ncRNA
Reads supporting (aligned to +/- strand): ref base C (5/1); new base T (27/65); total (33/66)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 101 total aligned reads displayed.

CACGCATCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTT  >  AE015451/1529930‑1530385

cacgcaTCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCgttgtc                                                                                                                                                                                                                                <  1:988029/234‑1 (MQ=255)

   gcaGCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCt                                                                                                                                                                                                                           <  1:503368/236‑1 (MQ=255)

    caTCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCt                                                                                                                                                                                                                           >  1:987685/1‑235 (MQ=255)

    caTCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTg                                                                                                                                                                                                                          >  1:795174/1‑236 (MQ=255)

                 gtgAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAAtt                                                                                                                                                                                                              <  1:495510/235‑1 (MQ=255)

                    aaGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  1:773750/235‑1 (MQ=255)

                    aaGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  1:507152/235‑1 (MQ=255)

                     aGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATCCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  2:1224799/234‑1 (MQ=255)

                      ggtggtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  2:987680/233‑1 (MQ=255)

                      ggtggtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  2:1164118/233‑1 (MQ=255)

                       gtggtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAAtt                                                                                                                                                                                                              <  2:453968/229‑1 (MQ=255)

                       gtggtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTAt                                                                                                                                                                                                           <  2:861229/232‑1 (MQ=255)

                          gtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGt                                                                                                                                                                                                    <  1:1171200/236‑1 (MQ=255)

                          gtCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGt                                                                                                                                                                                                    <  1:1002681/236‑1 (MQ=255)

                            cGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGtat                                                                                                                                                                                                  >  1:889506/1‑236 (MQ=255)

                            cGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGCCAGGCTGATTAATGGAATTTATCAGGGGtat                                                                                                                                                                                                  >  1:1174164/1‑236 (MQ=255)

                              aCAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGtata                                                                                                                                                                                                 <  1:708023/235‑1 (MQ=255)

                                aaTACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTTCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGtata                                                                                                                                                                                                 <  2:1197743/233‑1 (MQ=255)

                                     ccTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGCATAAGGGGGAt                                                                                                                                                                                         >  2:265903/1‑236 (MQ=255)

                                              tGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATtca                                                                                                                                                                                <  1:431583/236‑1 (MQ=255)

                                               gCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGa                                                                                                                                                                                          >  2:1094388/1‑225 (MQ=255)

                                                cccAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCGGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGt                                                                                                                                                                                                    <  1:435013/214‑1 (MQ=255)

                                                 ccAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATtcatca                                                                                                                                                                             >  2:781950/1‑236 (MQ=255)

                                                 ccAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATtca                                                                                                                                                                                >  2:591007/1‑233 (MQ=255)

                                                   agcagGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATtcatca                                                                                                                                                                             >  2:1243607/1‑234 (MQ=255)

                                                       ggCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGt                                                                                                                                                                        <  1:125082/235‑1 (MQ=255)

                                                           tACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTggg                                                                                                                                                                    >  1:1230415/1‑235 (MQ=255)

                                                                aGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               <  1:729400/235‑1 (MQ=255)

                                                                 gCGTCCAGTCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCgttgtc                                                                                                                                                                                                                                <  2:1168800/169‑1 (MQ=255)

                                                                  cGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTAGGTCTGTTa                                                                                                                                                             >  2:1194842/1‑235 (MQ=255)

                                                                   gTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  2:974523/188‑1 (MQ=255)

                                                                     ccAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTatca                                                                                                                                                          >  1:706264/1‑235 (MQ=255)

                                                                      cAAGCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCaaa                                                                                                                                                                           <  1:178754/217‑1 (MQ=255)

                                                                           cTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                                   <  1:781955/236‑1 (MQ=255)

                                                                           cTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                                   <  1:265907/236‑1 (MQ=255)

                                                                              cgcCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGAGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGcc                                                                                                                                                 <  1:1215668/235‑1 (MQ=255)

                                                                               gcCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCtt                                                                                                                                               >  1:1119463/1‑236 (MQ=255)

                                                                                 cGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATtc                                                                                                                                                                                 >  2:1003328/1‑200 (MQ=255)

                                                                                 cGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTttg                                                                                                                                             >  1:651989/1‑236 (MQ=255)

                                                                                   gTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCaaa                                                                                                                                                                           <  2:1037760/204‑1 (MQ=255)

                                                                                     gcgcCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               <  2:600180/214‑1 (MQ=255)

                                                                                      cgcCAGGAGCAGCCAGCGGTCACCTACCGTGACCGGGAGCGCCCGACCGTCATGCGCAACCAGGGGCATGCAGGTGCTGCCGAAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                                   <  1:285650/225‑1 (MQ=255)

                                                                                                            aCTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTg                                                                                                                   <  1:1244481/235‑1 (MQ=255)

                                                                                                               aCCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  2:388390/144‑1 (MQ=255)

                                                                                                                 cGTGACCTGGAGCGCCAGACCGTCATGCGCACCCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCg                                                                                                              <  1:340104/235‑1 (MQ=255)

                                                                                                                 cGTGACCTGCAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               <  1:19552/186‑1 (MQ=255)

                                                                                                                     aCCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                         <  1:425921/236‑1 (MQ=255)

                                                                                                                     aCCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCaa                                                                                                         <  1:701176/236‑1 (MQ=255)

                                                                                                                       cTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               <  2:634436/180‑1 (MQ=255)

                                                                                                                             cgcCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTg                                                                                                                                   >  2:386265/1‑202 (MQ=255)

                                                                                                                              acccGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGAt                                                                                                <  2:889501/235‑1 (MQ=255)

                                                                                                                               gccGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTgcg                                                                                                                      <  1:136993/212‑1 (MQ=255)

                                                                                                                               cccGAACGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAAGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCt                                                                                                                    <  2:105934/215‑1 (MQ=255)

                                                                                                                                 cGACCGTCATGCGCAATCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAGCCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTgg                                                                                                                                                                     <  2:687359/164‑1 (MQ=255)

                                                                                                                                 cGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               <  1:61377/170‑1 (MQ=255)

                                                                                                                                  gACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTAt                                                                                                                                                                                                           <  1:993700/125‑1 (MQ=255)

                                                                                                                                  gACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTGATCATcc                                                                                                                                                       >  2:174693/1‑177 (MQ=255)

                                                                                                                                   aCCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                                   <  1:1239462/180‑1 (MQ=255)

                                                                                                                                   aCCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaac                                                                                           <  1:191218/236‑1 (MQ=255)

                                                                                                                                    ccGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAg                                                                                                                                                   <  2:198622/179‑1 (MQ=255)

                                                                                                                                    ccGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaac                                                                                           <  1:46284/235‑1 (MQ=255)

                                                                                                                                     cGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                          <  1:302932/235‑1 (MQ=255)

                                                                                                                                            cgcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                                  <  1:1073804/236‑1 (MQ=255)

                                                                                                                                             gcAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCt                                                                                 <  1:143374/236‑1 (MQ=255)

                                                                                                                                               aaCCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAAttt                                                                                                                         <  2:768263/194‑1 (MQ=255)

                                                                                                                                                  cAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                          <  1:1185814/222‑1 (MQ=255)

                                                                                                                                                  cAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                          >  2:1185809/1‑222 (MQ=255)

                                                                                                                                                  cAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTgaaga                                                                            <  1:315782/236‑1 (MQ=255)

                                                                                                                                                   aGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                          <  2:178196/221‑1 (MQ=255)

                                                                                                                                                    ggCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCAGGATTAAACAACGCACCCTGAAGAAt                                                                          >  1:820708/1‑236 (MQ=255)

                                                                                                                                                       gcATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                                  <  1:543238/225‑1 (MQ=255)

                                                                                                                                                       gcATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATAcc                                                                       <  1:577836/236‑1 (MQ=255)

                                                                                                                                                        cATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTaa                                                                                             >  2:571801/1‑213 (MQ=255)

                                                                                                                                                           gCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTaa                                                                                             <  1:224976/210‑1 (MQ=255)

                                                                                                                                                              ggTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAggc                                                                                                                                                                        >  1:286073/1‑131 (MQ=255)

                                                                                                                                                               gTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                                      <  1:109840/213‑1 (MQ=255)

                                                                                                                                                                tgctgcCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                          <  2:108379/208‑1 (MQ=255)

                                                                                                                                                                 gctgcCGCAGCCGCTAAACTGAGCCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTggt                                                                                                                                                                    <  2:142312/133‑2 (MQ=255)

                                                                                                                                                                  ctgcCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCCCCCTGAAGAATACCATCCGTGcccc                                                            >  2:57195/1‑234 (MQ=255)

                                                                                                                                                                     ccGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTGGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGGATTCATCAAAGGTTgg                                                                                                                                                                     >  2:421511/1‑128 (MQ=255)

                                                                                                                                                                        cAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAATGCAc                                                                                    >  1:1270549/1‑206 (MQ=255)

                                                                                                                                                                          gCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGc                                                                                      <  1:987961/202‑1 (MQ=255)

                                                                                                                                                                                  aCTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCAc                                            <  1:1094393/236‑1 (MQ=255)

                                                                                                                                                                                        gccTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCggg                                      <  1:534463/235‑1 (MQ=255)

                                                                                                                                                                                              ggATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               <  1:567082/109‑1 (MQ=255)

                                                                                                                                                                                              ggATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGt                                                                                                                                                               >  2:567077/1‑109 (MQ=255)

                                                                                                                                                                                              ggATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGc                                                               <  2:1104828/205‑1 (MQ=255)

                                                                                                                                                                                                      tGGACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTaa                                                                                             >  2:647209/1‑167 (MQ=255)

                                                                                                                                                                                                        ggcTACCGCGATAGCCCGGCATTACTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGGCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAg                                                          <  2:386682/198‑1 (MQ=255)

                                                                                                                                                                                                        gACTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGa                      >  1:395759/1‑236 (MQ=255)

                                                                                                                                                                                                         aCTACCTCGATATCCCGGCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGAc                     <  1:591012/236‑1 (MQ=255)

                                                                                                                                                                                                                cGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTCATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAaaca                                                                                          >  2:929314/1‑160 (MQ=255)

                                                                                                                                                                                                                      cccGGCACTACTGCGTTGTCAGGCTGATTAATGGACTTCATCAGGGGTACAAAGGTGATTGGTATTCCTCAAAGGTTGGGTCTGCTATCATCCCCATCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAccc                                                                                  <  1:889833/162‑1 (MQ=255)

                                                                                                                                                                                                                        cGGCATTCCTGCGTTGTCAGGCTGATTAATGGAAtt                                                                                                                                                                                                              <  2:870549/36‑1 (MQ=255)

                                                                                                                                                                                                                          gCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGgt                                                        >  2:1083718/1‑184 (MQ=255)

                                                                                                                                                                                                                          gCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCt                                                >  2:667386/1‑192 (MQ=255)

                                                                                                                                                                                                                          gCATTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGCATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCAcc                                                                                   >  2:10782/1‑157 (MQ=255)

                                                                                                                                                                                                                            aTTCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGtt  >  1:602517/1‑236 (MQ=255)

                                                                                                                                                                                                                             ttCCTGCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCa                                             <  2:1103397/192‑1 (MQ=255)

                                                                                                                                                                                                                                  gCGTTGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCgg                                       <  2:482406/193‑1 (MQ=255)

CACGCATCGAGAAGCCTGTGAAGGTGGTCGACAATACCCTGCAGACTGCCCAGCAGGCGTACGAAGCGTCCAATCCTGCGCCGGTGCGCCAGGAGCAGCCAGCGGTCAACTACCGTGACCTGGAGCGCCCGACCGTCATGCGCAACCAGGCGCATGCAGGTGCTGCCGCAGCCGCTAAACTGAACCCTCAGGATGACCTGGACTACCTCGATATCCCGGCATTCCTGCGTCGTCAGGCTGATTAATGGAATTTATCAGGGGTATAAAGGTGATTGGTATTCATCAAAGGTTGGGTCTGTTATCATCCCCAGCCTTTGTTGATACCTGTTCGCAATTTGCGCTGAAGCGGCCAATGCCATGATTAAACAACGCACCCTGAAGAATACCATCCGTGCCACAGGTGTCGGTCTGCACTCCGGGGAGAAGGTTTACCTGACCCTCAAGCCTGCACCTGTT  >  AE015451/1529930‑1530385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`