| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,705,425 | G→A | 12.5% | intergenic (‑163/+14) | kdpF ← / ← PP_4162 | potassium‑transporting ATPase F/conserved membrane protein of unknown function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,705,425 | 0 | G | A | 12.5% | 84.6 / 5.7 | 32 | intergenic (‑163/+14) | kdpF/PP_4162 | potassium‑transporting ATPase F/conserved membrane protein of unknown function |
| Reads supporting (aligned to +/- strand): ref base G (20/8); new base A (2/2); total (22/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
GCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGTCCACACCGCTGATGACG > AE015451/4705321‑4705649 | gCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGCGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATtgttgt < 1:926131/128‑1 (MQ=255) ggtgggtATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTc > 1:832717/1‑166 (MQ=255) cGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGcc > 2:945948/1‑64 (MQ=255) cGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAGAGGcc < 1:945953/64‑1 (MQ=255) cGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCact > 1:26934/1‑167 (MQ=255) gTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGcc > 1:978720/1‑152 (MQ=255) gTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTAACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTAc > 2:663492/1‑135 (MQ=255) tAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCa > 2:1108515/1‑174 (MQ=255) gTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGa > 2:599443/1‑182 (MQ=255) ttGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAg < 1:1191845/233‑1 (MQ=255) tCTGCCTGTAACGGCCTCTTCGCGGACTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAg > 1:724893/1‑126 (MQ=255) gCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAac > 2:1191840/1‑174 (MQ=255) cTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTGCAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGa > 2:815745/1‑131 (MQ=255) tAACGGCCCTTTCGCGGGCTTGCCCGCGAAAGGGCCGCTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGGCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAAGCCAATGAAg > 1:1230783/1‑145 (MQ=39) aCGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTa > 1:649410/1‑38 (MQ=255) aCGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCcag > 2:792416/1‑175 (MQ=255) ccTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATc < 1:1108520/211‑1 (MQ=255) ccTCTTCGCGGGCAAGCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAg < 1:488075/213‑1 (MQ=255) tctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGTCcaca < 1:664632/236‑1 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGcc < 2:1258445/129‑1 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGcc > 1:1258450/1‑129 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGaa > 2:524132/1‑159 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGa > 2:1171120/1‑158 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAaca > 2:1106620/1‑161 (MQ=255) ctTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGcc > 2:663084/1‑165 (MQ=255) gggCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACAGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTgccgc > 1:693206/1‑191 (MQ=255) caagCCCGCGAAGAGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGa > 2:488070/4‑149 (MQ=255) tGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGTCCACAcc < 1:663089/226‑1 (MQ=255) ccGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGctgctg > 2:462079/1‑43 (MQ=255) ccGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACAGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGTCCACACCGCTGATGACg < 2:693201/233‑1 (MQ=255) gcgAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGt < 2:1093439/214‑1 (MQ=255) gcgAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGt > 1:1093444/1‑214 (MQ=255) | GCGTAAAGGGGCGAGATCGGGGGGTGGGGGTGGGTATAAAGAATGCGTAAAAATCAGCGTTGTGGTCTGCCTGTAACGGCCTCTTCGCGGGCTTGCCCGCGAAAGGGCCGTTACAGGCTTATTGTTGTGGCGGCTGCTGCGCCGCGTTACGGCTCCAGTCCAGCAGCAGGCTGTAGCCTACCGCCAGCAAGGTCGGCCCGATGAAAAGGCCAATGAAGCCAAAGGCAAGCAAGCCGCCGAACACGCCCAGCAGCACGATCACCAGCGGCAGGTTGCCGCCACGGCTGATCAGGTACGGTTTGAGCACGTTGTCCACACCGCTGATGACG > AE015451/4705321‑4705649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |