Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 6,132,525 T→C 17.8% E443G (GAG→GGG)  copA‑II ← copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154516,132,5250TC17.8% 164.6 / 5.7 74E443G (GAG→GGG) copA‑IIcopper resistance protein A
Reads supporting (aligned to +/- strand):  ref base T (38/21);  new base C (9/5);  total (47/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTTAGGCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGCCGTGAT  >  AE015451/6132294‑6132735
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  
aaCTTAGGCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGc                                                                                                                                                                                                                >  1:1116233/1‑236 (MQ=255)
  cTTAGGCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGcc                                                                                                                                                                                                               <  1:1102551/235‑1 (MQ=255)
       gCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCAtttt                                                                                                                                                                                                          <  1:572048/235‑1 (MQ=255)
               cTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCAtct                                                                                                                                                                                                                    >  2:1197990/1‑217 (MQ=255)
               cTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCAtct                                                                                                                                                                                                                    <  1:1197995/217‑1 (MQ=255)
               cTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGa                                                                                                                                                                                                   >  1:790574/1‑234 (MQ=255)
                     gTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCAtct                                                                                                                                                                                                                    >  1:4823/1‑211 (MQ=255)
                                                    ctGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCCGCCAtt                                                                                                                                                            >  1:406478/1‑236 (MQ=255)
                                                         gCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCa                                                                                                                                                                                               <  2:559091/196‑1 (MQ=255)
                                                          cTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCTTGGTCCATCTCAGCCAtttt                                                                                                                                                                                                          >  2:1094216/1‑184 (MQ=255)
                                                          cTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCtgtg                                                                                                                                                                                                    >  2:808963/1‑190 (MQ=255)
                                                                           gcgcCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCgg                                                                                                                                                                                         <  2:961055/184‑1 (MQ=255)
                                                                                  ccATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTCTGGTCCATACCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGCTCCATGCCGGCCATGCCGCCGTGGTCCATCTCCGCCa                                                                                                                                                                                                              >  1:849711/1‑156 (MQ=255)
                                                                                    aTACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGcc                                                                                                                                                                                       >  1:200005/1‑177 (MQ=255)
                                                                                         ttCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGc                                                                                                                                                        >  1:964200/1‑203 (MQ=255)
                                                                                         ttCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGc                                                                                                                                >  1:89182/1‑227 (MQ=255)
                                                                                             aTGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCgg                                                                                                                                                                                         >  2:638810/1‑166 (MQ=255)
                                                                                              tgctgcTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATccc                                                                                                                   >  1:1104731/1‑235 (MQ=255)
                                                                                               gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTTcc                                                                                                                               >  1:990094/1‑222 (MQ=255)
                                                                                               gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAt                                                                                                                                                                                     >  1:431547/1‑168 (MQ=255)
                                                                                               gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAg                                                                                                                 >  1:653990/1‑236 (MQ=255)
                                                                                               gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGGCg                                                                                                                                                                                          >  2:653780/1‑163 (MQ=255)
                                                                                                     gtgGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCgg                                                                                                                                                                                         >  2:1170131/1‑158 (MQ=255)
                                                                                                      tgGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGt                                                                                                                                        >  1:881765/1‑206 (MQ=255)
                                                                                                      tgGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAttt                                                                                                           >  1:386574/1‑235 (MQ=255)
                                                                                                              atCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATACCGGTCATGCTGCCCTCGTCCACCCCAGCCAtttt                                                                                                                                                          >  2:785859/1‑180 (MQ=14)
                                                                                                               tCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATcc                                                                                                                    <  1:960415/217‑1 (MQ=255)
                                                                                                                cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGt                                                                                                  >  1:679591/1‑234 (MQ=255)
                                                                                                                 ggCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCtgtg                                                                                                                                                                                                    <  2:595417/135‑1 (MQ=255)
                                                                                                                 ggCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAtgctg                                                                                                                                                                                 <  2:63388/154‑1 (MQ=255)
                                                                                                                     aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGcc                                                                                                                                                                                       <  2:371516/144‑1 (MQ=255)
                                                                                                                     aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCgg                                                                                                                                         >  1:225724/1‑190 (MQ=255)
                                                                                                                     aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGc                                                                                                                                >  1:1172017/1‑199 (MQ=255)
                                                                                                                     aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCGTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCt                                                                                                                                                                                                       >  2:276892/1‑128 (MQ=255)
                                                                                                                         cgccTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGTTCCATGCCGGCCATGCTGCCATGGGCCATCCCAGCCATTTTGCtggg                                                                                                                                                    >  1:372025/1‑173 (MQ=255)
                                                                                                                          gccTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGcc                                                                                                                                                                                       >  2:1211412/1‑139 (MQ=255)
                                                                                                                          gccTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATCTTGCTGTGGTCCATTCCGGTCATGTTGc                                                                                                                                >  1:85196/1‑194 (MQ=255)
                                                                                                                               ggTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                                                                       <  1:172654/182‑1 (MQ=255)
                                                                                                                               ggTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCa                                                                                                                       >  1:265240/1‑198 (MQ=255)
                                                                                                                               ggTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCAGGGTCCATCCCAGCCATTTTTCtg                                                                                                                                                      >  1:310372/1‑167 (MQ=255)
                                                                                                                                              tCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATccc                                                                                                                                                                   >  2:516754/1‑139 (MQ=255)
                                                                                                                                              tCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGc                                                                                        >  1:485216/1‑214 (MQ=255)
                                                                                                                                                 tGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                                                                       <  1:889716/164‑1 (MQ=255)
                                                                                                                                                  gCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCg                                                                                                                                          >  2:489365/1‑160 (MQ=255)
                                                                                                                                                  gCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGCTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCcgt                                                                                                                                                     >  2:79438/1‑149 (MQ=255)
                                                                                                                                                    ggtGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt                                                                                                     <  2:485211/193‑1 (MQ=255)
                                                                                                                                                               tCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                                                            <  1:84048/193‑1 (MQ=255)
                                                                                                                                                                cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                          >  1:21831/1‑226 (MQ=255)
                                                                                                                                                                cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTAATCCATGCCGGCCAtg                                                                                                                                                                                    >  1:25572/1‑104 (MQ=255)
                                                                                                                                                                   ccATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                                                                          <  2:1116228/127‑1 (MQ=255)
                                                                                                                                                                     aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCCGCCATTTTGCTGTGGTCCATGCCGGCCCTGCTGCCGTGGTCCATCCCCGACCTTTTGCTGTGGTGCATggc                                                                                                                                           >  2:702112/1‑138 (MQ=14)
                                                                                                                                                                     aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCAt                                                                                                                      >  2:977534/1‑161 (MQ=255)
                                                                                                                                                                      tGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                                          <  1:937769/172‑1 (MQ=255)
                                                                                                                                                                          gccTTGGTCCATCCCATCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                                                                       <  2:905101/139‑1 (MQ=255)
                                                                                                                                                                          gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTc                                                                                                                         >  2:727396/1‑153 (MQ=255)
                                                                                                                                                                               ggTCCATCCCATCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTcc                                                                                                                        <  2:458435/149‑1 (MQ=255)
                                                                                                                                                                                   cATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGcc                                                                                                                                                               <  2:294738/106‑1 (MQ=255)
                                                                                                                                                                                   cATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt                                                                                                     <  1:817345/164‑1 (MQ=255)
                                                                                                                                                                                         aGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAttt                                                                                                           >  1:795173/1‑152 (MQ=255)
                                                                                                                                                                                              tCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTg                                                                                 >  2:131892/1‑173 (MQ=255)
                                                                                                                                                                                                    tctgGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGcc                                                                                                                                                                                           <  2:141791/59‑1 (MQ=255)
                                                                                                                                                                                                       ggTCCATGCCGGCCATGCCGCCGGGGTCCATCGCAGCCATTTTGCTGTGATCCAGGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                                                            <  2:97611/153‑1 (MQ=255)
                                                                                                                                                                                                        gTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGc                                                                                        >  2:661810/1‑156 (MQ=255)
                                                                                                                                                                                                           cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCTGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCgt                                         >  1:906352/1‑199 (MQ=255)
                                                                                                                                                                                                           cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCa                                                                                                              >  2:180215/1‑131 (MQ=255)
                                                                                                                                                                                                           cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTCGTCCATCCCAGCCAtt                                                            >  2:359277/1‑181 (MQ=255)
                                                                                                                                                                                                              gCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCCtgtctcttatac  >  1:347128/1‑226 (MQ=255)
                                                                                                                                                                                                              gCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATACCGGCCAtgctg                                                                                                                                                                                 <  2:173629/61‑1 (MQ=255)
                                                                                                                                                                                                                cGGCCATGCCGCCGTGGTCCATCTCCGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCCGTGGTCCATGCCGGCCATGTTGCCGTGGTCCACACCAg                                                                                                                 >  2:1038041/1‑123 (MQ=14)
                                                                                                                                                                                                                cGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCCTTTTGCTGTGGTCCATGCCGGTCATGATGCCGTGGTCCATcc                                                                                                                    >  2:302860/1‑120 (MQ=255)
                                                                                                                                                                                                                 ggCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATcc                                                                    <  2:1096808/167‑1 (MQ=255)
                                                                                                                                                                                                                  gCCATGCCGCCGTGGTCCAACTCACCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                                                                          <  1:493248/80‑1 (MQ=255)
                                                                                                                                                                                                                         cgccgTCGTCCATCCCATCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGc                                                                                        <  2:1197612/139‑1 (MQ=11)
                                                                                                                                                                                                                          gccgTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATcccg                                                                                                                  >  2:563338/1‑111 (MQ=255)
                                                                                                                                                                                                                           ccgTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                                                                                                            <  2:849706/85‑1 (MQ=255)
                                                                                                                                                                                                                               cgTCCATCTCAGCCATTTTGCTGTGTTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                          <  2:984364/162‑1 (MQ=255)
                                                                                                                                                                                                                                 cccATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                                          <  2:239014/112‑1 (MQ=255)
                                                                                                                                                                                                                                    acctcAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGtt                                  <  1:276896/180‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  
AACTTAGGCGTTGGACTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGCCGTGAT  >  AE015451/6132294‑6132735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: