Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 6,132,542 A→G 13.6% D437D (GAT→GAC)  copA‑II ← copper resistance protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE0154516,132,5420AG13.6% 173.7 / 4.2 70D437D (GAT→GAC) copA‑IIcopper resistance protein A
Reads supporting (aligned to +/- strand):  ref base A (37/21);  new base G (4/5);  total (43/27)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

CTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGCCGTGATCCATACCCATATCACTCATTGA  >  AE015451/6132309‑6132757
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                       
cTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGa                                                                                                                                                                                                                         >  1:790574/1‑234 (MQ=255)
                                     ctGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCCGCCAtt                                                                                                                                                                                  >  1:406478/1‑236 (MQ=255)
                                          gCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCa                                                                                                                                                                                                                     <  2:559091/196‑1 (MQ=255)
                                                            gcgcCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCgg                                                                                                                                                                                                               <  2:961055/184‑1 (MQ=255)
                                                                     aTACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGcc                                                                                                                                                                                                             >  1:200005/1‑177 (MQ=255)
                                                                          ttCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGc                                                                                                                                                                              >  1:964200/1‑203 (MQ=255)
                                                                          ttCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGc                                                                                                                                                      >  1:89182/1‑227 (MQ=255)
                                                                              aTGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCgg                                                                                                                                                                                                               >  2:638810/1‑166 (MQ=255)
                                                                               tgctgcTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATccc                                                                                                                                         >  1:1104731/1‑235 (MQ=255)
                                                                                gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTTcc                                                                                                                                                     >  1:990094/1‑222 (MQ=255)
                                                                                gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAt                                                                                                                                                                                                           >  1:431547/1‑168 (MQ=255)
                                                                                gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAg                                                                                                                                       >  1:653990/1‑236 (MQ=255)
                                                                                gctgctGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGGCg                                                                                                                                                                                                                >  2:653780/1‑163 (MQ=255)
                                                                                      gtgGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCgg                                                                                                                                                                                                               >  2:1170131/1‑158 (MQ=255)
                                                                                       tgGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGt                                                                                                                                                              >  1:881765/1‑206 (MQ=255)
                                                                                       tgGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAttt                                                                                                                                 >  1:386574/1‑235 (MQ=255)
                                                                                               atCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATACCGGTCATGCTGCCCTCGTCCACCCCAGCCAtttt                                                                                                                                                                                >  2:785859/1‑180 (MQ=14)
                                                                                                tCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATcc                                                                                                                                          <  1:960415/217‑1 (MQ=255)
                                                                                                 cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGt                                                                                                                        >  1:679591/1‑234 (MQ=255)
                                                                                                  ggCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCAtgctg                                                                                                                                                                                                       <  2:63388/154‑1 (MQ=255)
                                                                                                      aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGcc                                                                                                                                                                                                             <  2:371516/144‑1 (MQ=255)
                                                                                                      aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCgg                                                                                                                                                               >  1:225724/1‑190 (MQ=255)
                                                                                                      aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGc                                                                                                                                                      >  1:1172017/1‑199 (MQ=255)
                                                                                                          cgccTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGTTCCATGCCGGCCATGCTGCCATGGGCCATCCCAGCCATTTTGCtggg                                                                                                                                                                          >  1:372025/1‑173 (MQ=255)
                                                                                                           gccTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGcc                                                                                                                                                                                                             >  2:1211412/1‑139 (MQ=255)
                                                                                                           gccTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATCTTGCTGTGGTCCATTCCGGTCATGTTGc                                                                                                                                                      >  1:85196/1‑194 (MQ=255)
                                                                                                                ggTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                                                                                             <  1:172654/182‑1 (MQ=255)
                                                                                                                ggTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCa                                                                                                                                             >  1:265240/1‑198 (MQ=255)
                                                                                                                ggTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCAGGGTCCATCCCAGCCATTTTTCtg                                                                                                                                                                            >  1:310372/1‑167 (MQ=255)
                                                                                                                               tCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCTTGGTCCATccc                                                                                                                                                                                         >  2:516754/1‑139 (MQ=255)
                                                                                                                               tCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGc                                                                                                              >  1:485216/1‑214 (MQ=255)
                                                                                                                                  tGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                                                                                             <  1:889716/164‑1 (MQ=255)
                                                                                                                                   gCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCg                                                                                                                                                                >  2:489365/1‑160 (MQ=255)
                                                                                                                                   gCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGCTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCcgt                                                                                                                                                                           >  2:79438/1‑149 (MQ=255)
                                                                                                                                     ggtGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt                                                                                                                           <  2:485211/193‑1 (MQ=255)
                                                                                                                                                tCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                                                                                  <  1:84048/193‑1 (MQ=255)
                                                                                                                                                 cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                >  1:21831/1‑226 (MQ=255)
                                                                                                                                                 cGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTAATCCATGCCGGCCAtg                                                                                                                                                                                                          >  1:25572/1‑104 (MQ=255)
                                                                                                                                                    ccATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                                                                                                <  2:1116228/127‑1 (MQ=255)
                                                                                                                                                      aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCCGCCATTTTGCTGTGGTCCATGCCGGCCCTGCTGCCGTGGTCCATCCCCGACCTTTTGCTGTGGTGCATggc                                                                                                                                                                 >  2:702112/1‑138 (MQ=14)
                                                                                                                                                      aTGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCAt                                                                                                                                            >  2:977534/1‑161 (MQ=255)
                                                                                                                                                       tGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                                                                <  1:937769/172‑1 (MQ=255)
                                                                                                                                                           gccTTGGTCCATCCCATCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTc                                                                                                                                                             <  2:905101/139‑1 (MQ=255)
                                                                                                                                                           gccTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTc                                                                                                                                               >  2:727396/1‑153 (MQ=255)
                                                                                                                                                                ggTCCATCCCATCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTcc                                                                                                                                              <  2:458435/149‑1 (MQ=255)
                                                                                                                                                                    cATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGcc                                                                                                                                                                                     <  2:294738/106‑1 (MQ=255)
                                                                                                                                                                    cATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCtgt                                                                                                                           <  1:817345/164‑1 (MQ=255)
                                                                                                                                                                          aGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAttt                                                                                                                                 >  1:795173/1‑152 (MQ=255)
                                                                                                                                                                               tCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTg                                                                                                       >  2:131892/1‑173 (MQ=255)
                                                                                                                                                                                     tctgGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGcc                                                                                                                                                                                                                 <  2:141791/59‑1 (MQ=255)
                                                                                                                                                                                        ggTCCATGCCGGCCATGCCGCCGGGGTCCATCGCAGCCATTTTGCTGTGATCCAGGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                                                                                  <  2:97611/153‑1 (MQ=255)
                                                                                                                                                                                         gTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGc                                                                                                              >  2:661810/1‑156 (MQ=255)
                                                                                                                                                                                            cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCTGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCgt                                                               >  1:906352/1‑199 (MQ=255)
                                                                                                                                                                                            cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCa                                                                                                                                    >  2:180215/1‑131 (MQ=255)
                                                                                                                                                                                            cATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTCGTCCATCCCAGCCAtt                                                                                  >  2:359277/1‑181 (MQ=255)
                                                                                                                                                                                               gCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCCtgtctcttatac                        >  1:347128/1‑226 (MQ=255)
                                                                                                                                                                                               gCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATACCGGCCAtgctg                                                                                                                                                                                                       <  2:173629/61‑1 (MQ=255)
                                                                                                                                                                                                 cGGCCATGCCGCCGTGGTCCATCTCCGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCCGTGGTCCATGCCGGCCATGTTGCCGTGGTCCACACCAg                                                                                                                                       >  2:1038041/1‑123 (MQ=14)
                                                                                                                                                                                                 cGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCCTTTTGCTGTGGTCCATGCCGGTCATGATGCCGTGGTCCATcc                                                                                                                                          >  2:302860/1‑120 (MQ=255)
                                                                                                                                                                                                  ggCCATGCCGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATcc                                                                                          <  2:1096808/167‑1 (MQ=255)
                                                                                                                                                                                                   gCCATGCCGCCGTGGTCCAACTCACCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCAtttt                                                                                                                                                                                <  1:493248/80‑1 (MQ=255)
                                                                                                                                                                                                          cgccgTCGTCCATCCCATCCATTTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGc                                                                                                              <  2:1197612/139‑1 (MQ=11)
                                                                                                                                                                                                           gccgTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATcccg                                                                                                                                        >  2:563338/1‑111 (MQ=255)
                                                                                                                                                                                                            ccgTGGTCCATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGc                                                                                                                                                                  <  2:849706/85‑1 (MQ=255)
                                                                                                                                                                                                                cgTCCATCTCAGCCATTTTGCTGTGTTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                <  2:984364/162‑1 (MQ=255)
                                                                                                                                                                                                                  cccATCCCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCAtttt                                                                                                                                <  2:239014/112‑1 (MQ=255)
                                                                                                                                                                                                                     acctcAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGtt                                                        <  1:276896/180‑1 (MQ=255)
                                                                                                                                                                                                                         cAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGCCGTGATCCATACCCATATCACTCATtga  >  1:42510/1‑232 (MQ=255)
                                                                                                                                                                                                                             cATCTTGCTGTGGTCCATGCCGGCCATGCTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGcc                                                                                     <  2:851676/145‑1 (MQ=255)
                                                                                                                                                                                                                              aTTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCa                                                                                                            >  2:895589/1‑121 (MQ=255)
                                                                                                                                                                                                                                      gtgATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGcc                                                             <  1:423509/160‑1 (MQ=255)
                                                                                                                                                                                                                                         aTCCATGCCGGCCATGCTGCCATGGTCCATCCCGGCCTTTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTg                                                       <  1:325533/163‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                       
CTCATAGTCTGCATGTCGACCAAGGGGTTGTTAGTCTCTGAGGCTGGGTGAGACTGCATGGCGCCACCCATACCTTCCATGCTGCTGTGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTATGGTCCATATCGGCCATGCCGCCTTGGTCCATCCCAGCCATCTTGCTGTGGTCCATGCCGGCCATGCCGCCGTGGTCCATCTCAGCCATTTTGCTGTGATCCATGCCGGCCATGCTGCCATGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGTCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATGCCGGCCATGTTGCCGTGGTCCATCCCAGCCATTTTGCTGTGGTCCATACCGGCCATGTTGCCGTGGTCCATGCCGGCCATGCTGCCGTGATCCATACCCATATCACTCATTGA  >  AE015451/6132309‑6132757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑