BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	197,179	A→G	9.4%	V895V (GTA→GTG)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	197,179	0	A	G	9.4%	415.4 / 21.1	150	V895V (GTA→GTG)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand): ref base A (53/81); new base G (5/9); total (59/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

Only 100 of 152 total aligned reads displayed.

GCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCC  >  AE015451/196944‑197384

gCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTa                                                                                                                                                                                                               >  1:590446/1‑236 (MQ=38)

        atcaCCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          <  1:847456/230‑1 (MQ=2)

             cGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCg                                                                                                                                                                                                  >  1:921753/1‑236 (MQ=25)

                   aGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCt                                                                                                                                                                                                        >  2:537977/1‑224 (MQ=12)

                    gCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCt                                                                                                                                                                                           <  1:537982/236‑1 (MQ=16)

                          aaGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGTCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCa                                                                                                                                                                                      <  1:797826/235‑1 (MQ=39)

                                   ggCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCa                                                                                                                                                                            <  1:792361/236‑1 (MQ=21)

                                      aGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc                                                                                                                                                                                                            <  2:921748/201‑1 (MQ=21)

                                         gTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  1:40477/236‑1 (MQ=18)

                                         atCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:886676/235‑1 (MQ=38)

                                           cgacttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          >  2:1125496/5‑198 (MQ=255)

                                           cgacttCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGc                                                                                                                                                                       >  2:1107581/5‑233 (MQ=255)

                                           ccaTTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                <  2:1186876/206‑1 (MQ=255)

                                           cGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                >  1:1186881/1‑208 (MQ=40)

                                                 ccAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCg                                                                                                                                                              <  1:361143/236‑1 (MQ=39)

                                                     aCCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCt                                                                                                                                                                                           <  2:821092/203‑1 (MQ=25)

                                                     aCCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCt                                                                                                                                                                                           >  1:821097/1‑203 (MQ=40)

                                                       cccGGCCACTGACGCCTACAACCACGGTTCGACCGTCCGCGTCCCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          <  2:116385/186‑1 (MQ=11)

                                                        ccGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACg                                                                                                                                                                        <  1:603613/219‑1 (MQ=21)

                                                           gCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGGCACCGAAGGCGGCGTAATCACCTACACCGCGACCCCGAGCa                                                                                                                                                                                      >  1:287385/1‑202 (MQ=255)

                                                           gCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTc                                                                                                                                                    <  1:1009141/236‑1 (MQ=12)

                                                            cGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGAAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          <  2:1077507/181‑1 (MQ=39)

                                                            cGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGAAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          >  1:1077512/1‑181 (MQ=39)

                                                             gAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTaca                                                                                                                                                                                                     >  1:80507/1‑185 (MQ=25)

                                                             gAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTac                                                                                                                                                                                                      >  1:992426/1‑184 (MQ=255)

                                                             gAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTac                                                                                                                                                                                                      <  2:992421/184‑1 (MQ=255)

                                                             gAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCa                                                                                                                                                  >  1:1073825/1‑236 (MQ=255)

                                                                tGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg                                                                                                                                               <  1:581557/236‑1 (MQ=40)

                                                                  aCGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACg                                                                                                                                               <  1:1088853/234‑1 (MQ=40)

                                                                    gTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:242848/209‑1 (MQ=38)

                                                                          aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATc                                                                                                                                                                                                            <  1:723825/165‑1 (MQ=38)

                                                                             aacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca                                                                                                                                                                                                     <  2:1158340/169‑1 (MQ=37)

                                                                                  ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTacac                                                                                                                                                                                                    >  1:325376/1‑165 (MQ=40)

                                                                                  ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca                                                                                                                                                                                                     <  2:1265716/164‑1 (MQ=255)

                                                                                  ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGcc                                                                                                                                            >  1:1013531/1‑221 (MQ=255)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCaaaccattac                                                                                                                                  >  1:820893/1‑220 (MQ=19)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          <  1:749915/157‑1 (MQ=255)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:614481/193‑1 (MQ=39)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  1:873004/193‑1 (MQ=38)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATCACCGTTGa                                                                                                                            >  1:468198/1‑235 (MQ=255)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc                                                                                                                                                                      >  2:523750/1‑193 (MQ=37)

                                                                                    cGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  1:523755/193‑1 (MQ=255)

                                                                                            aGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACg                                                                                                                                                                        <  2:1054975/183‑1 (MQ=37)

                                                                                              cGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGt                                                                                                                                                     <  2:640500/200‑1 (MQ=255)

                                                                                               gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                                                                                                                                                <  1:802724/156‑1 (MQ=9)

                                                                                               gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTg                                                                                                                                                                                                >  2:802719/1‑156 (MQ=18)

                                                                                               gTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGc                                                                                                                                                                                       <  2:325371/165‑1 (MQ=35)

                                                                                               gTCACCATCGAGAACGCCACACGCGGTAAATTCGGGCCGCTCACCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg                                                                                                                                                                                        <  1:974777/164‑1 (MQ=11)

                                                                                                 cACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGcc                                                                                                                                                                              >  1:1047216/1‑172 (MQ=35)

                                                                                                    cATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGcc                                                                                                                                            >  1:665198/1‑203 (MQ=40)

                                                                                                        gagaACGCCACGGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCCCCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa                                                                                                                                                                 <  1:669973/178‑1 (MQ=9)

                                                                                                           aaCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGCCCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGa                                                                                                                                                           <  2:393807/181‑1 (MQ=39)

                                                                                                            aCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCa                                                                                                                                                                                      <  2:418931/153‑1 (MQ=14)

                                                                                                                cacaGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCa                                                                                                                                                                                                           <  2:1004502/128‑1 (MQ=39)

                                                                                                                  caGGCGGTAATTTCGAGCAACTGCCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGGCCGTCGGTCACCGAAGGCGGCGTAATCACGTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGtga                                                                                                                                                                 <  1:1134278/168‑3 (MQ=255)

                                                                                                                             ttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      >  1:1207011/1‑152 (MQ=34)

                                                                                                                                  gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCaaac                                                                                                                                        <  1:624040/177‑4 (MQ=2)

                                                                                                                                  gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          >  2:1254052/1‑111 (MQ=38)

                                                                                                                                  gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCAc                                                                                                                                                                                                          <  1:1254057/111‑1 (MQ=38)

                                                                                                                                  gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCaaac                                                                                                                                        >  2:624035/1‑174 (MQ=25)

                                                                                                                                    aaCTGACCCCGAACCCGACCCCGGCTCAGACCCCGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCaa                                                                                                                                                                                     <  1:243919/130‑1 (MQ=21)

                                                                                                                                                    gACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                <  2:970774/103‑1 (MQ=35)

                                                                                                                                                        ccGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:815571/125‑1 (MQ=32)

                                                                                                                                                        ccGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACAGTGACCCTGTCCAACGGcc                                                                                                                                            >  1:25940/1‑151 (MQ=14)

                                                                                                                                                        ccGGCTCAGACCACGATCAACGACTCGGTCGACGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGcc                                                                                                                                                                              <  1:183016/117‑1 (MQ=17)

                                                                                                                                                            cTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGtca                                                                                                                                      >  2:936626/1‑153 (MQ=18)

                                                                                                                                                               aGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                >  2:570534/1‑92 (MQ=33)

                                                                                                                                                               aGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTg                                                                                                                                                            >  1:923469/1‑128 (MQ=35)

                                                                                                                                                                  ccACGATCAACGACTCGGTCGACCCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCg                                                                                                                       >  1:1059716/1‑162 (MQ=255)

                                                                                                                                                                         cAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca                                                                                                                                                                                                     >  1:158810/1‑77 (MQ=34)

                                                                                                                                                                              aCTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCt                                                                                                                                                                                           >  1:1169038/1‑82 (MQ=33)

                                                                                                                                                                              aCTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCTTGTCCAACGGCCAAGCCATCACCGTTGAAGCCGGTAAAACCCAGGGCAGCGTCGAATTc                                                                                             >  2:1170140/1‑176 (MQ=11)

                                                                                                                                                                                  ggTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATc                                                                                                                                                                                                            >  2:167558/1‑61 (MQ=35)

                                                                                                                                                                                  ggTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATc                                                                                                                                                                                                            <  1:167561/61‑1 (MQ=35)

                                                                                                                                                                                     cGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGcc                                                                                                                                            >  2:646752/1‑122 (MQ=35)

                                                                                                                                                                                      gATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCt                                                                                                                                                                                                        >  1:718365/1‑61 (MQ=37)

                                                                                                                                                                                      gATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCt                                                                                                                                                                                                        <  2:718360/61‑1 (MQ=37)

                                                                                                                                                                                             ccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCa                                                                                <  2:1059711/174‑1 (MQ=255)

                                                                                                                                                                                               accGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTaa                                                                                                                                                                 >  1:633539/1‑91 (MQ=33)

                                                                                                                                                                                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAAt                                                                                                                                                                                                             >  1:82584/1‑45 (MQ=38)

                                                                                                                                                                                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:1255326/84‑1 (MQ=35)

                                                                                                                                                                                                 cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      >  1:1255331/1‑84 (MQ=33)

                                                                                                                                                                                                    gACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTaca                                                                                                                                                                                                     >  2:1020970/1‑50 (MQ=38)

                                                                                                                                                                                                     aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      >  1:605914/1‑80 (MQ=35)

                                                                                                                                                                                                     aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:605909/80‑1 (MQ=35)

                                                                                                                                                                                                     aCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCaaa                                                                                                                                         >  2:1283325/1‑107 (MQ=25)

                                                                                                                                                                                                      cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                <  1:477239/53‑1 (MQ=38)

                                                                                                                                                                                                      cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                >  2:477234/1‑53 (MQ=35)

                                                                                                                                                                                                        cTGACGGCGAGCCCGTCGGTCACCGAAGTCGCCGTTATAACCATCACCGTGACCCCGAGCAATCCTGCCCAGACGCCGGCCACCGTCACCCTGTCCAAc                                                                                                                                                >  1:475118/1‑99 (MQ=11)

                                                                                                                                                                                                        cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACAcc                                                                                                                                                                                                   <  2:506882/48‑1 (MQ=38)

                                                                                                                                                                                                          gACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTg                                                                                                                                                                                                <  1:446674/49‑1 (MQ=38)

                                                                                                                                                                                                              gCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAATCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGACCAGCTGAccc  >  1:772954/1‑235 (MQ=255)

                                                                                                                                                                                                                   cgcGTCGGTCACCTAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  2:108610/64‑1 (MQ=255)

                                                                                                                                                                                                                    ccGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCa                                                                                                                                                                                      <  2:793228/49‑1 (MQ=38)

                                                                                                                                                                                                                         ggTCACCGAAGGCGGCGTAATCTACTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc                                                                                                                                                                      <  1:1090029/60‑1 (MQ=11)

                                                                                                                                                                                                                         ggTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAg                                                                                                                                                                           <  2:290438/55‑1 (MQ=35)

                                                                                                                                                                                                                          gTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGt                                                                                                                                                     <  2:457158/76‑1 (MQ=40)

                                                                                                                                                                                                                             aCCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAAt                                                                              <  2:631480/144‑1 (MQ=40)

                                                                                                                                                                                                                                 aaGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCa                                                                                                                                                                            <  2:200551/46‑1 (MQ=38)

                                                                                                                                                                                                                                        cgTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCa                                                                                                                                                                            <  2:726522/39‑1 (MQ=40)

GCCAAACCATTACCGTTGAAGCCGGCAAGACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGCAACTTCGAACAGCTGACCC  >  AE015451/196944‑197384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`