| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 199,915 | T→C | 9.1% | F1807F (TTT→TTC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 199,915 | 0 | T | C | 9.1% | 235.5 / 5.8 | 74 | F1807F (TTT→TTC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (42/25); new base C (4/3); total (46/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCG > AE015451/199695‑200147 | agaTCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAaacctgaacctg > 1:939840/1‑236 (MQ=255) ccTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGc < 1:1152222/235‑1 (MQ=255) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAgg > 1:591634/1‑235 (MQ=40) aacaaAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGt > 1:205372/1‑236 (MQ=40) gTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACaa > 1:1108055/1‑205 (MQ=255) tCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGc < 1:470088/236‑1 (MQ=255) cTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGcc > 1:898700/1‑236 (MQ=255) tGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGc > 2:1141966/1‑234 (MQ=39) cGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGAc > 2:920042/1‑197 (MQ=255) aaCCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAgg > 1:693592/1‑236 (MQ=255) aCTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCg > 2:1261047/1‑194 (MQ=40) aGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAaacctgaacctg > 2:1237623/1‑176 (MQ=255) cTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCg < 1:749640/235‑1 (MQ=255) gCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGt > 2:396453/1‑177 (MQ=255) gACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGt > 2:1178391/1‑174 (MQ=255) gACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCa > 1:949594/1‑175 (MQ=255) gACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCa < 2:949589/175‑1 (MQ=255) gACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAgg > 2:249670/1‑206 (MQ=255) aCGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACAACGTaaa > 1:324097/1‑226 (MQ=255) accacTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCAc < 1:79125/227‑1 (MQ=255) ccacTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGcc < 1:168060/236‑1 (MQ=255) acTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGcc < 2:446278/234‑1 (MQ=255) tGTCGTCGTGGCAGCCAACAGCACCACCGGCTCGGCGACTGCAACCGCCCCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGAc < 1:665282/235‑1 (MQ=255) tcgtcgTGGCAGCCAACAGCACCACGGGCGCGGCGGCTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGc < 2:535787/185‑1 (MQ=255) gtcgtGGCAGCCACCAGCCCCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAaacctg < 2:296119/135‑1 (MQ=255) tcgtGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGCACACCAGGc > 2:1211862/1‑191 (MQ=18) cgtGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACt > 1:732956/1‑235 (MQ=255) cAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCg < 2:939835/233‑1 (MQ=255) gCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTCGAAAACCTGAACCTGGACAAGAcccc < 2:887115/143‑1 (MQ=14) gCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTCGAAAACCTGAACCTGGACAAGAcccc > 1:887120/1‑143 (MQ=37) ccAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGc < 1:1047065/235‑1 (MQ=255) ccacGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGAACCCGGTCAGCACCTAGGTAACCGACGAGCCAGGCACTCCAGGCAACGAAGGc > 2:596488/1‑182 (MQ=255) gggCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAAc > 1:207601/1‑236 (MQ=255) gCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGAGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCAT‑GGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTc < 1:862787/225‑1 (MQ=12) gACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCtt > 2:618927/1‑234 (MQ=255) gACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCa > 1:335802/1‑236 (MQ=255) aCTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTCCAc > 1:754843/1‑236 (MQ=255) cAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTc < 2:207598/218‑1 (MQ=255) gACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACTGACCTTCACCGTGCACGTCAAcc > 1:710052/1‑235 (MQ=255) cAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGc < 1:670885/236‑1 (MQ=255) cTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGc > 2:1129542/1‑122 (MQ=17) tATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTg > 1:703085/1‑216 (MQ=255) tCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGAGGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc < 1:1045030/236‑1 (MQ=255) cGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGccc > 2:791802/1‑233 (MQ=18) cGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCAc > 1:488300/1‑235 (MQ=255) ccAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGCCTTCg > 2:554734/1‑173 (MQ=255) ccAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTGACCGACGAGCCAGGCACACCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGGCTTCGGTGGCCGAGAACGTCAAACCGAcc > 1:3374/1‑197 (MQ=18) ccAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCaa > 1:910447/1‑214 (MQ=255) gcCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCt > 2:165769/1‑191 (MQ=40) gTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGc > 1:67885/1‑81 (MQ=38) cagcGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAAt < 1:371178/232‑1 (MQ=37) cATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACg > 2:1278755/1‑164 (MQ=39) cATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt > 2:959551/1‑225 (MQ=38) aTCGACGCGGTCAGCGGCGCAGATGCAT‑GGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATg < 1:502093/235‑1 (MQ=12) tCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca < 1:546746/228‑1 (MQ=37) cGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATc > 2:1056727/1‑130 (MQ=35) aCGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTc > 2:400956/1‑62 (MQ=35) aCGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTc > 2:653017/1‑234 (MQ=40) aCGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg > 1:1166885/1‑236 (MQ=255) aCGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg < 1:165772/236‑1 (MQ=40) gcgGTCAGCGGCGCAGATGCGTGGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACg < 2:865913/203‑1 (MQ=255) tCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGca < 2:854320/219‑1 (MQ=39) aGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCAt > 1:876189/1‑236 (MQ=255) cggcgCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCa < 1:276907/236‑1 (MQ=40) cgcAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCa > 2:813398/1‑233 (MQ=255) aGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCt < 1:1261052/235‑1 (MQ=40) cGT‑GGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATGGTCAAGGTCACCATCACTGCCggc > 2:159957/1‑114 (MQ=11) t‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggcca < 1:536945/197‑6 (MQ=255) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGc < 1:1169360/39‑1 (MQ=40) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGcc > 2:1131358/1‑59 (MQ=35) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGc > 1:1054850/1‑81 (MQ=35) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAg > 1:992994/1‑236 (MQ=255) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAg > 1:1254181/1‑236 (MQ=255) gTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgcg < 1:249674/236‑1 (MQ=255) | AGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACTGTCGTCGTGGCAGCCAACAGCACCACGGGCTCGGCGACTGCAACCGCACCAGACAACGTCTATGTCGGCACCAACGCGCCGGTGATCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGT‑GGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCG > AE015451/199695‑200147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |