| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 200,113 | T→C | 8.9% | N1873N (AAT→AAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 200,113 | 0 | T | C | 8.9% | 227.9 / 6.9 | 74 | N1873N (AAT→AAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (33/34); new base C (3/4); total (36/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCG > AE015451/199879‑200348 | cagcGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAAt < 1:371178/232‑1 (MQ=37) aTCGACGCGGTCAGCGGCGCAGATGCATGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGTACGCCAGGCAACGAAGGCGATATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATg < 1:502093/235‑1 (MQ=12) aCGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTc > 2:653017/1‑234 (MQ=40) aCGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg > 1:1166885/1‑236 (MQ=255) aCGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg < 1:165772/236‑1 (MQ=40) aGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCAt > 1:876189/1‑236 (MQ=255) cggcgCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCa < 1:276907/236‑1 (MQ=40) cgcAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCa > 2:813398/1‑233 (MQ=255) aGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCt < 1:1261052/235‑1 (MQ=40) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAg > 1:992994/1‑236 (MQ=255) aaGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAg > 1:1254181/1‑236 (MQ=255) gTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgcg < 1:249674/236‑1 (MQ=255) cgAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCaa > 2:888595/2‑216 (MQ=11) aaaCCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATg > 2:325759/1‑197 (MQ=35) acatgaacctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCTGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCGCAg > 1:706912/4‑200 (MQ=18) aacctgGACAAGACCCCGGGCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGACGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgc < 1:216301/222‑1 (MQ=255) acctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTgg < 1:609235/211‑1 (MQ=38) ctgGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCCCTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCa < 1:184624/202‑1 (MQ=38) aCCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAgg < 2:898695/236‑1 (MQ=255) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGAt < 1:328856/236‑1 (MQ=255) cGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGAt < 1:618932/236‑1 (MQ=255) cGGCCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGAt < 1:532741/236‑1 (MQ=255) tgctgaCCGGGGTTACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGAt > 1:901614/6‑221 (MQ=255) tgctgaCCGGGGTTACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGAt < 2:901609/216‑1 (MQ=255) aCCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCt < 1:959556/235‑1 (MQ=39) aGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGATCAACAATGATCAGCTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTa < 2:693587/232‑1 (MQ=255) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGaa < 2:816324/185‑1 (MQ=255) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGaa > 1:816329/1‑185 (MQ=255) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGCAAc > 2:386337/1‑187 (MQ=255) tCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAgg < 2:992989/234‑1 (MQ=255) ccacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCa < 1:92249/183‑1 (MQ=255) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAg < 2:906899/159‑1 (MQ=40) gacgaGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCg > 1:146576/1‑192 (MQ=40) gCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCCCCATCCCGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCa < 2:277695/153‑1 (MQ=255) cACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCa < 2:488295/233‑1 (MQ=255) cTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGAt < 2:968335/191‑1 (MQ=40) cAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCa < 1:1030714/228‑1 (MQ=255) aGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTgggt < 1:596493/235‑2 (MQ=255) gCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAACCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCACGGCTGCCGAAACCAGCGCGCCg > 2:126106/1‑173 (MQ=255) gtctaCATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGTCCTTCACCGTGCACGTCAACCACCCGCTGGCCAACGACCTGGTCGTAACCCTGAGCAACAATACTCAGGTCACAATCAAGGCt > 2:1163422/5‑147 (MQ=11) cGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAg > 2:994693/1‑130 (MQ=25) cGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAg < 1:994698/130‑1 (MQ=25) ggTCACCATCACGGCCGACCAGACTTCGGTAGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACACCCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAg > 1:646836/1‑144 (MQ=11) aCCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGtg > 1:475535/1‑235 (MQ=255) aTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGa > 2:1196779/1‑221 (MQ=255) aTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGtgct > 2:793031/1‑234 (MQ=255) aTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGggctgc > 1:217142/1‑236 (MQ=255) aTCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCTCAGGTCACCATCAAGGCTGGCGAACCCAGCGCGCCGCACACCCACGCGGCGCAAGGc > 2:376461/1‑165 (MQ=255) aCGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTg < 1:920047/198‑1 (MQ=40) ggCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGtt < 1:1178396/234‑1 (MQ=255) ccGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGACgcgc > 2:1192530/1‑153 (MQ=14) ccGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAg > 2:1261851/1‑156 (MQ=14) aCCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAAc > 1:558332/1‑236 (MQ=255) cAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAAc > 2:601388/1‑234 (MQ=255) cAGACTTCGGTAGCCGAGGACGTCAAGCCGACCTTCACCATGCACGTCAACCAGCCGCTGACCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGtgctg > 2:715502/1‑223 (MQ=255) cGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgc < 2:343625/119‑1 (MQ=32) ccGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCgg > 1:482948/1‑235 (MQ=255) gaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAg > 1:665844/1‑108 (MQ=33) gaACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAg < 2:665839/108‑1 (MQ=33) aaCCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGtt < 1:791807/236‑1 (MQ=255) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgcg > 2:621165/1‑84 (MQ=35) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAgcg < 1:621170/84‑1 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCa > 1:1044426/1‑97 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCa < 2:1044421/97‑1 (MQ=33) aCGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCt > 1:879614/1‑235 (MQ=255) cGTCAACCAGCCGCTGGCCTACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCa < 1:219698/135‑1 (MQ=255) tGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGcc < 2:988729/120‑1 (MQ=12) gCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGcc > 2:574557/1‑234 (MQ=255) aCCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTGCTGCTTCGGTTCAAGTCAc < 1:1031850/202‑1 (MQ=9) tCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGcc < 2:732951/221‑1 (MQ=255) tGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCgaga < 2:754838/156‑1 (MQ=255) cccTGAGCAACAACGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGa < 1:992975/130‑1 (MQ=255) cTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGAc > 2:755221/1‑129 (MQ=255) cTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCg > 2:787857/1‑234 (MQ=255) caacaaTGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCGGCTTCGGt > 1:264900/1‑174 (MQ=255) tGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCg > 1:464766/1‑236 (MQ=255) | CATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTTGAAAACCTGAACCTGGACAAGACCCCGGTCAGCACCGAGGTCACCGACGAGCCAGGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACCGTGCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAATGCTCAGGTCACCATCAAGGCTGGCGAAACCAGCGCGCCGTACACCCACGATGCGCAAGGCGATGACGTCTATCAGGACGCTGGCCAGATCAGCCTGGGCATCAACTCGGCAGTAGACGCCACTGGTGCTGCGTTCGAGAACCTTGAGCTGGGCGGTTCCGCTTCGGTTCAAGTCACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCCACCCCTTCGGTGACCGAAGGCG > AE015451/199879‑200348 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |