| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 202,457 | A→G | 6.4% | T2655A (ACC→GCC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 202,457 | 0 | A | G | 6.4% | 311.5 / 2.3 | 96 | T2655A (ACC→GCC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (50/39); new base G (2/5); total (52/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACC > AE015451/202228‑202687 | gCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGccc > 1:500882/1‑236 (MQ=38) ggcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGt > 1:7194/1‑236 (MQ=255) tGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAAcgcg < 1:1237725/236‑1 (MQ=0) cacCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCggtgg < 1:737992/236‑1 (MQ=255) tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACTACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCggtg > 1:77237/1‑230 (MQ=255) tggtggCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGAc < 1:1122147/236‑1 (MQ=255) gtggCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg < 2:1005813/186‑1 (MQ=255) gtggCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg > 1:1005818/1‑186 (MQ=255) gtggCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGAc > 2:1107644/1‑234 (MQ=40) aaGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAAc > 2:744793/1‑173 (MQ=35) gACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCggt > 2:1268848/1‑207 (MQ=40) cgccTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTACAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAAt > 2:894076/3‑206 (MQ=25) aCCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGCTGCGTGGAAGTTCGa > 1:861350/1‑236 (MQ=21) ccTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAg < 1:1107649/236‑1 (MQ=37) ccTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAg < 1:9517/236‑1 (MQ=35) cTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAg < 1:511144/235‑1 (MQ=37) acacCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGc > 2:1239346/1‑184 (MQ=33) cacCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCggt > 2:274811/1‑165 (MQ=18) aTCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCTATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGAcccc < 1:1071810/233‑1 (MQ=25) aTCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGt > 1:900460/1‑236 (MQ=37) ccTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGc < 1:1008288/235‑1 (MQ=37) cTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTACGTCGGCACCAACGCGCCGGTGGTCAATACAATCGACGCCGTCAGCGGCGCAGAGGc > 1:64673/1‑191 (MQ=255) cTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGAAAACGTCTATGTCGGCACCa > 2:283974/1‑145 (MQ=21) cTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGCACGACGTCTACGTCGGCACCAAcgac > 1:794832/1‑148 (MQ=255) ccAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCcggcggcc > 2:316323/1‑153 (MQ=18) ccAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCa > 2:1010359/1‑156 (MQ=33) ccAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGaa > 1:204705/1‑193 (MQ=34) ccAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCGGa > 2:753585/1‑207 (MQ=21) caacaaAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGCCGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGt < 2:593766/171‑1 (MQ=25) aacaaAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGt > 1:214562/1‑236 (MQ=35) aacaaAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGt > 1:719634/1‑236 (MQ=37) acaaAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTgg < 2:647416/210‑1 (MQ=38) gACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg < 2:28157/111‑1 (MQ=33) gACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCgg > 2:1036543/1‑128 (MQ=35) ccGGTCTGCCGATCAACACCCACGCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg < 2:302517/109‑1 (MQ=255) aCGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAAcc < 2:11006/195‑1 (MQ=34) aCGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTg < 2:879295/197‑1 (MQ=35) cGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACg > 1:914538/1‑236 (MQ=40) ggTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGa < 1:951995/236‑1 (MQ=38) gTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg > 2:1157485/1‑107 (MQ=33) gTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg < 1:1157490/107‑1 (MQ=33) gTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAg > 1:1092122/1‑236 (MQ=40) cTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGa > 1:1252869/1‑221 (MQ=37) cTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGa < 2:1252864/221‑1 (MQ=38) tGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGcc < 1:881572/235‑1 (MQ=39) cGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAgg > 1:742576/1‑235 (MQ=40) ccACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAg < 2:914533/233‑1 (MQ=255) aCTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGCGGTCAATGCCATCGACGCCGTCAgcggc > 2:289737/1‑149 (MQ=37) ggAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGaaa > 2:1188810/1‑235 (MQ=255) ggAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCCCCggt > 2:311399/1‑117 (MQ=25) gAGCTGTACTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGa > 2:1173658/1‑209 (MQ=2) tGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTccg > 2:972179/1‑134 (MQ=37) tACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGactgcctctt > 1:367726/1‑227 (MQ=255) aCTTCAAGCTGACCGATGGCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCg < 2:440287/231‑1 (MQ=9) ttCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTg > 2:481653/1‑146 (MQ=37) ttCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGCCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTc > 2:539994/1‑195 (MQ=255) tCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAAc > 1:590122/1‑98 (MQ=39) gCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCaag > 1:761754/1‑234 (MQ=255) tGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCa > 2:966434/1‑180 (MQ=39) tGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCaaa > 2:727393/1‑233 (MQ=255) ccGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCa > 2:1080426/1‑234 (MQ=255) ccGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCAc < 1:727398/235‑1 (MQ=255) gatggCACTACTGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCgg < 1:729276/166‑1 (MQ=9) gCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTAct < 1:1025671/236‑1 (MQ=255) ccccGTCGTCGTGGCAGCCAACAGCCCCCCCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAAcgcg < 1:124256/80‑1 (MQ=255) ccGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGcc < 1:1173663/184‑1 (MQ=255) cgtcgtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGaa < 2:1124991/141‑1 (MQ=38) cgtcgtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCg < 1:949804/212‑1 (MQ=255) gtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGtt < 1:1275101/125‑1 (MQ=17) gtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGc < 2:452592/178‑1 (MQ=255) gtcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGa < 1:1080431/235‑1 (MQ=255) tcgtGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGAc > 1:342858/1‑235 (MQ=255) gtGGCAGCCAACAGCACCACCGGTTCGGCCACTGTAGCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCGGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACTGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGata > 1:565932/1‑202 (MQ=2) ggCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGAcc < 2:742571/232‑1 (MQ=255) cAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCaaa < 1:936881/206‑1 (MQ=255) gCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGc > 2:949799/1‑174 (MQ=255) aGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAgg < 1:521595/185‑1 (MQ=255) aGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAgg > 2:521590/1‑185 (MQ=255) ccccGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTgg < 1:274815/120‑1 (MQ=255) ccGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTAAGCACCGAGGTc > 2:613335/1‑146 (MQ=255) cGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAACCTGGACAAGACCCCGGTCAGCAc > 1:230507/1‑138 (MQ=21) ggTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc < 1:1272488/236‑1 (MQ=255) gTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCGATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTTGATCTGGACAAGACCCCGGTCAGCACGGAGGTCACCGACGAGCCGCGCACTCCAGGCAACGAAGGCGACATCGTCAAGGTCAcc > 2:1215601/1‑193 (MQ=11) gTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAAcc > 1:394350/1‑235 (MQ=255) gTTCGGCCACTGCAACCGCCCCGAACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACTTGGATCTGGACAAGACCCCGGTCAGCACCg > 1:567706/1‑138 (MQ=255) tCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGAc < 1:744798/236‑1 (MQ=255) cGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGAc < 2:1080302/235‑1 (MQ=255) ggCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTc < 1:428179/181‑1 (MQ=255) ggCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCt < 1:83281/236‑1 (MQ=255) gcccccgCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTc < 1:184478/219‑1 (MQ=255) gCCACTGCAACCGACCCGGACAACGCCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCATCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTGaaat > 1:681347/1‑228 (MQ=255) cccggCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGtt < 2:290349/89‑1 (MQ=255) aCTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCa > 2:1043655/1‑184 (MQ=255) aCTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGAGATCGTCa > 2:906654/1‑178 (MQ=255) tGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCAcc < 1:861072/235‑1 (MQ=255) agCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGc < 2:367721/147‑1 (MQ=255) aaCCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTc < 2:240060/172‑1 (MQ=255) | GCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCAATGCCATCGACGCCGTCAGCGGCGCAGATGCGTGGAAGTTCGAAAACCTGAATCTGGACAAGACCCCGGTCAGCACCGAGGTCACTGACGAGCCAGGCACTCCAGGCAACGAAGGCGATATCGTCAAAGTCACTATTACTGCTGACCAGACCTCGGTGGCCGAGAACGTCAAACCGACCTTCACC > AE015451/202228‑202687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |