| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 216,949 | T→C | 6.3% | D7485D (GAT→GAC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 216,949 | 0 | T | C | 6.3% | 239.5 / 4.8 | 77 | D7485D (GAT→GAC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (40/32); new base C (3/2); total (43/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01 | |||||||||||
CGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGA > AE015451/216729‑217181 | cgcAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATcaa < 1:749273/235‑1 (MQ=34) ggCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAAcc < 1:432250/235‑1 (MQ=34) ggCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAAcc < 1:526261/235‑1 (MQ=37) gACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGc < 1:1268281/236‑1 (MQ=40) cGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGc > 2:1080884/1‑234 (MQ=38) aCAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCCACCCCTTCGGTGACCGAAGGCGGCGAGATCACTTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAAcc > 1:850521/1‑222 (MQ=2) aTGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATc < 2:361506/209‑1 (MQ=32) cTGGCCAGATCAGCCGCGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCGTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCAt < 2:890815/222‑1 (MQ=255) tGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTg > 1:825671/1‑235 (MQ=255) tGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTg > 1:1047111/1‑235 (MQ=255) gCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCa > 1:700492/1‑200 (MQ=32) aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTCACCGAAGGTGGCGAGATCACTTACACCATCACCCTGACCAACAAAGACGGCCTGc < 1:395392/190‑1 (MQ=9) tCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGATGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACGTACACCATCACCCTGGCCAACAAAGATGGTCTGCCAATCAACAACAACAGt > 2:686099/1‑197 (MQ=255) agcTCGGCGGTGGACGACCCTGGTGCGACCTTCGAGAACCTCGAGCGGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAg < 1:384456/213‑1 (MQ=255) cTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTAGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATc < 2:694145/181‑1 (MQ=21) cTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATc > 1:694150/1‑181 (MQ=34) cTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCg < 1:1017746/236‑1 (MQ=255) ggcggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATc < 2:182591/178‑1 (MQ=33) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGc > 1:376508/1‑171 (MQ=31) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTg < 2:655915/233‑1 (MQ=255) ggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGTGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGGCCCCTTCGCTAACGGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAAcc < 2:693673/182‑1 (MQ=255) ggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGACGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTCACCTTCACGCtc > 2:65136/1‑204 (MQ=255) tGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTg > 2:734533/1‑181 (MQ=255) ttCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGCGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGcct > 1:390284/1‑167 (MQ=255) ttCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATg > 1:1010626/1‑142 (MQ=32) ttCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATg < 2:1010621/142‑1 (MQ=32) ttCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACgg > 2:1216632/1‑221 (MQ=255) ttCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACt > 2:1199769/1‑234 (MQ=255) gccGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGa < 2:25391/170‑1 (MQ=255) agaACCGCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGc < 2:72823/144‑1 (MQ=17) aaCCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCg < 1:1080889/235‑1 (MQ=255) cGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGt > 1:37862/1‑154 (MQ=255) cGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGt < 2:37861/154‑1 (MQ=255) cGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTg > 1:140475/1‑236 (MQ=255) cGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTg < 1:486267/236‑1 (MQ=255) gTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCCCCg > 1:569615/1‑204 (MQ=255) gcgAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTAcc > 1:56804/1‑213 (MQ=255) cgAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTc < 2:492176/233‑1 (MQ=255) aGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAACATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTc < 1:744847/236‑1 (MQ=255) ggTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACg < 2:448252/233‑1 (MQ=255) gATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTAc < 1:1199774/236‑1 (MQ=255) tCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCg < 1:275588/212‑1 (MQ=39) cGACACCACCGACGATGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGACGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGAc < 1:712161/235‑1 (MQ=255) acacCACCGACGTAGTGGTTGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGCTCACCTACACCATCACCCTGCTCAACAAAGATGGTCTGCCCATCAACAACCACAGTGCATTGAcc > 2:106123/1‑128 (MQ=14) acacCACCGACGTAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGATCAACAAAGATGGTCTGCCAATCAACAACACCAGTGCATTGACCTTCACGCTGAGCGAccg > 2:106122/1‑143 (MQ=255) cgacgaAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc > 1:572881/1‑183 (MQ=39) cgacgaAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc < 2:572876/183‑1 (MQ=40) cgaggtggtgGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCAATCAACAACCACACGGCATTGAACTTCACGCTGACCGACGGCAAAACCGTCATCACCGTGCCga > 2:505001/5‑157 (MQ=11) cgaAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCCACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCAc > 2:41800/1‑168 (MQ=255) cgaAGTGGTGGCCAAACTGCCCGCCACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCg < 2:285908/152‑1 (MQ=255) gtggCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGa > 2:175813/1‑191 (MQ=38) tggCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAatcgatc < 2:691532/233‑1 (MQ=39) ggCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGAcc > 2:855304/1‑215 (MQ=255) ccAAGCTGACCGCAACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGATGGTCTGCCGATCAACAACcacgc > 1:791135/1‑95 (MQ=11) tGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTggc > 1:188351/1‑236 (MQ=39) gACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCt < 1:929375/72‑1 (MQ=35) ccTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGATGGTCTGCAGATCAACAACcac > 2:693930/1‑78 (MQ=11) ccTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGt > 2:316769/1‑176 (MQ=38) cGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCATGCCGGCCAACgg > 1:34430/1‑133 (MQ=37) tAACCGAAGGCGGCGAGATCACCAACACCATCACCCTGACCAACAAAGGTGGTCTGCCAATCAACAACCACAGTACAGTGACCTGCACGCTGAGCGACGGCTAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCg < 1:879667/150‑1 (MQ=11) gAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCATGTGATCAAat > 2:1247732/1‑196 (MQ=38) cggcgAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCTGTACCa > 1:18285/1‑173 (MQ=25) ggcgAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGt > 2:233959/1‑140 (MQ=34) cTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGa < 2:432201/59‑1 (MQ=35) aTCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGAcccc < 1:762026/236‑1 (MQ=255) cccTGACCAACAAAGTTGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCACCGGCCCCGTGGGCACTGCTACCGTCCCTGCCCCGGACAACGTCGACGt > 2:442986/1‑142 (MQ=11) cccTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCCTTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGAACCTGTGATCAAATCGATCGCAACCgct > 2:308323/1‑180 (MQ=21) cccTGACCAACAAAGATGGCCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCAcc < 1:445344/83‑1 (MQ=18) cTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCa > 1:898587/1‑235 (MQ=255) ccAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGCCCCTGTGATCAAatcgat > 2:178691/1‑166 (MQ=18) aacaaAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGGCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGAGGTTGGCAAGTTCGAAc > 1:119050/1‑202 (MQ=255) aacaaAGATGGTCTGCCAATCAACAACCACAGTGCATTCACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCCACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGt > 2:53895/1‑128 (MQ=17) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGTGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCa > 2:566241/1‑229 (MQ=255) aacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCa < 1:566246/229‑1 (MQ=255) aaGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTGGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTAACACTGGACAAGACCCCGGTCAGCAccgcc > 1:1205472/1‑233 (MQ=255) aGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGa > 2:1178343/1‑180 (MQ=40) aGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGa > 1:278111/1‑236 (MQ=255) | CGCAGGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCCGCGAAGGTGGATGTCACCGACACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACCCCTTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCAATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGTCACTGCCCCGGACAACGTCTACGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGGCGGCGCGGATGTTGGCAAGTTCGAACAGCTGACACTGGACAAGACCCCGGTCAGCACCGCCGTGA > AE015451/216729‑217181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |