| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,960,263 | C→A | 76.3% | G70C (GGC→TGC) | fliF ← | Flagellar M‑ring protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,960,263 | 0 | C | A | 76.3% | 96.2 / 29.9 | 42 | G70C (GGC→TGC) | fliF | Flagellar M‑ring protein |
| Reads supporting (aligned to +/- strand): ref base C (5/5); new base A (21/11); total (26/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01 | |||||||||||
GGTGCCCAGGCCCTGCTCCTTGTCGAGCAGTTCGAAGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTGCTTCAGCCATGGGTTACATCCGTCC > AE015451/4960054‑4960485 | ggTGCCCAGGCCCTGCTCCTTGTCGAGCAGTTCGAAGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGgttgt > 1:628880/1‑235 (MQ=255) ccccTTGTCGAGCAGTTCGAAGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATc < 1:229413/211‑1 (MQ=255) ccTTGTCGAGCAGTTCGAAGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACg > 1:346810/1‑236 (MQ=255) aaGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGgttgt < 1:244305/201‑1 (MQ=255) aGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACg > 1:303611/1‑236 (MQ=255) gcgcCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGa < 1:258064/231‑1 (MQ=255) gCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGgttgt < 2:168971/172‑1 (MQ=255) gcggcTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTg < 1:578167/236‑1 (MQ=255) aGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCgcag > 2:463257/1‑233 (MQ=255) gagaGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGtt < 1:463258/236‑1 (MQ=255) ccGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAgcggcg > 2:602267/1‑234 (MQ=255) aGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTg < 2:281229/175‑1 (MQ=255) gTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGc > 1:388843/1‑236 (MQ=255) aTGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACca > 2:106705/1‑92 (MQ=255) aTGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACca < 1:106705/92‑1 (MQ=255) aTGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATg > 1:96809/1‑177 (MQ=255) aTGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATg < 2:96809/177‑1 (MQ=255) aTGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGCGCCAg > 2:266598/1‑229 (MQ=255) aTGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGc > 1:584941/1‑236 (MQ=255) gTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCa > 2:20407/1‑164 (MQ=255) gTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGa > 2:536956/1‑184 (MQ=255) gTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCAGGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCg > 1:175485/1‑221 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGc < 1:384813/166‑1 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGc > 2:384812/1‑166 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCggggg > 1:288066/1‑236 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCgggg > 1:511813/1‑235 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCt > 2:230766/1‑117 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCt < 1:695926/146‑1 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCt > 2:695925/1‑146 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCAt > 2:85984/1‑162 (MQ=255) agcagcGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTGGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGAt > 2:412272/1‑144 (MQ=255) cAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTcga < 1:344153/236‑1 (MQ=255) aGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTcgac > 1:449931/1‑236 (MQ=255) gtgtCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATgg > 2:344152/1‑145 (MQ=255) tGACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCCGCATGGGCATCTGCga > 2:284287/1‑148 (MQ=255) gACCTGCTTGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTGCTTCAg < 1:602268/236‑1 (MQ=255) tGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGGGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTGCTTCAGCCATGGGt > 1:703144/1‑236 (MQ=255) tGGTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTg > 2:278876/1‑222 (MQ=255) ggTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGa > 2:109325/1‑54 (MQ=255) ggTGTCCATGCCCGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGa < 1:109325/54‑1 (MQ=255) ccGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTGCTTCAg < 2:303610/216‑1 (MQ=255) cGACAGGCTGCAGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTGCTTCAGCCATGGGTTACATCCGTcc < 2:388842/234‑1 (MQ=255) | GGTGCCCAGGCCCTGCTCCTTGTCGAGCAGTTCGAAGCCGACATTGCCGTCGCTCGGCGCCACGCCGGCGGCTGCCAGTTTCAGGCGCGCACGGGAGAGGTCGTCGGCCTTGACCAGCAGGGCCCCCGAGTTGGGCTCCACGTTATAGGGGATGTCAGCAGCGGCCAGGGTGTCCATGACCTGCTTGGTGTCCATGCCCGACAGGCTGCCGTACAACGGACGGTAATCGGGTTGTTGCGACCACAGCACCACGGCAAAGCCGATGGCCACGCTGGCCGCCAGGCCGACCAGGAGGCCGATCTGGCGCAGCATGGGCATCTGCGAGATGTTTTCCAGAAACGCCATGCCAAACAGCGGCGGCTTGGCCGCTGGCGAGCCAGCTTTGGCGGGGGCGTTTTCGACGACTGCTTCAGCCATGGGTTACATCCGTCC > AE015451/4960054‑4960485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |