| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 4,964,454 | C→T | 23.3% | D302N (GAC→AAC) | fleQ ← | Transcriptional regulator FleQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 4,964,454 | 0 | C | T | 23.3% | 77.9 / 25.7 | 43 | D302N (GAC→AAC) | fleQ | Transcriptional regulator FleQ |
| Reads supporting (aligned to +/- strand): ref base C (15/18); new base T (5/5); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01 | |||||||||||
ACGGGTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGAT > AE015451/4964226‑4964679 | aCGGGTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTcgcg > 1:560246/1‑235 (MQ=255) ggTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAg < 1:664845/236‑1 (MQ=255) ggTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAg < 1:63738/236‑1 (MQ=255) aTGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCt > 2:646536/1‑222 (MQ=255) aTGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCt < 1:646537/222‑1 (MQ=255) aTGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGcc < 1:190819/236‑1 (MQ=255) gcgTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTtgtg < 2:295434/231‑1 (MQ=255) cccGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTGCGAGGTTCTTGTGCGTCg > 2:501232/1‑226 (MQ=255) cAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAg < 2:550519/180‑1 (MQ=255) cAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAg > 1:550520/1‑180 (MQ=255) aGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACg < 1:141146/236‑1 (MQ=255) acaGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTgg < 1:172881/236‑1 (MQ=255) aTCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCtt > 2:426569/1‑188 (MQ=255) gAGGCGGAATTGAAGCGAATGTAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGcc < 2:304809/158‑1 (MQ=255) ggAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTggg > 1:460571/1‑203 (MQ=255) acccGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCt < 2:227391/202‑1 (MQ=255) ttCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGc > 1:743513/1‑202 (MQ=255) cgcGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCacac < 1:108242/234‑1 (MQ=255) gTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGACGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGAGCACGTCGATGCTTTGGGTCTTGTTGCTACCCaa > 1:465959/1‑203 (MQ=255) tGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCagcagc > 2:252368/1‑233 (MQ=255) gcgAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGAc > 1:79227/1‑235 (MQ=255) gAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCacac < 1:233561/220‑1 (MQ=255) tCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACGCGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCt < 2:208570/169‑1 (MQ=255) gTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCTTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTAc > 2:438804/1‑176 (MQ=255) agcaGCAACGGTATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAAc < 1:181219/229‑1 (MQ=255) tGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTtcg < 1:294684/236‑1 (MQ=255) cTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACg < 1:28147/165‑1 (MQ=255) tCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAggg < 1:252369/235‑1 (MQ=255) cACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAgg > 2:648895/1‑233 (MQ=255) cACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCGCACGCAGCAGCTTGACCTGCATCGGCAAc > 1:157639/1‑203 (MQ=255) cACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTa > 1:532678/1‑236 (MQ=255) cACGCAGCGCCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCg < 2:581181/86‑1 (MQ=255) ccATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGc > 1:166102/1‑235 (MQ=255) ttCGATAGGGAACACGTTCAGGCGGTAGTACAGGATTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCgatga > 2:590641/1‑95 (MQ=255) aTAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGtt < 1:561956/224‑1 (MQ=255) acGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCa < 2:730317/75‑1 (MQ=255) acGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCa > 1:730318/1‑75 (MQ=255) gTTCAGGCGTTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGGATCGGCAACGGCATGTCGCCGATTTCGTCGAg < 2:718507/195‑1 (MQ=255) aGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAgg < 2:631237/198‑1 (MQ=255) gCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCt > 1:58208/1‑234 (MQ=255) gCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCACAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCt > 2:404884/1‑234 (MQ=255) ggTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCgg > 1:653685/1‑225 (MQ=255) tagtaCAGGTTTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGAt < 1:648896/236‑1 (MQ=255) | ACGGGTGCATGATCGCCATGCGCTCGACCAGGTTGGCCAGCTCGCGTACGTTGCCCGGCCAGCCGTGACGACACAGCGACATGATCGAGGCGGAATTGAAGCGAATGGAACCGCGCTTCTCGTGCTCCATGCGCGAAATCAGCTCGTTCATCAGCAACGGGATGTCTTCCACCCGCTCACGCAGCGGCGCCATTTCGATAGGGAACACGTTCAGGCGGTAGTACAGGTCTTCGCGGAAGGTGCCGTCCTCGATCATGGTTTCGAGGTTCTTGTGCGTCGCAGCGATGATGCGCACGTCGATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGAT > AE015451/4964226‑4964679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |