Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AE015451 202,276 G→A 8.0% E2594E (GAG→GAA PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451202,2760GA 8.0% 177.1 / 4.4 62E2594E (GAG→GAAPP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base A (3/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCA  >  AE015451/202073‑202502
                                                                                                                                                                                                           |                                                                                                                                                                                                                                   
cTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGt                                                                                                                                                                                                     >  1:648263/1‑236 (MQ=255)
   aGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAc                                                                                                                                                                                                  <  1:647262/236‑1 (MQ=255)
         aacGCTCAGGTCACATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGc                                                                                                                                                                                           <  1:98229/236‑1 (MQ=255)
           cGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCg                                                                                                                                                                                          >  1:5538/1‑236 (MQ=255)
              tCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGt                                                                                                                                                                                        <  1:492674/235‑1 (MQ=255)
              tCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTg                                                                                                                                                                                       <  1:727168/236‑1 (MQ=255)
                aGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga                                                                                                                                                                                      >  1:426629/1‑235 (MQ=255)
                   tCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTg                                                                                                                                                                                       >  2:727167/1‑231 (MQ=255)
                                               gcCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga                                                                                                                                                                                      >  2:275784/1‑204 (MQ=40)
                                               gcCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTga                                                                                                                                                                                      <  1:275785/204‑1 (MQ=40)
                                                      ccccACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAc                                                                                                                                                    <  1:143471/230‑1 (MQ=37)
                                                      acCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTc                                                                                                                                                <  1:744455/235‑1 (MQ=39)
                                                         cACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCt                                                                                                                                               >  2:323370/1‑233 (MQ=40)
                                                                cgcAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCgg                                                                                                                                                                                         >  1:595800/1‑184 (MQ=39)
                                                                cgcAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGACACACCTTCGAGAACCTGCAACTGGGCGGCACCGCGACGGTTCAAGTGACCGACACCCCCGACGAAGTCGTAGCCAAGTTGACCGCCCCCCCGTCGGTCACCGAAGGCGGAGAGATCACCTACACCATCAcg                                                                                                                                                                   >  2:466809/1‑205 (MQ=11)
                                                                   aaGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACcaacaa                                                                                                                                                        >  2:356001/1‑214 (MQ=39)
                                                                                       gACGCTGGCCAGATCAGCTTGGGCATCACGTCGGCGGTGGATGTAGACGGTCCTGCGTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc                                                                                                                                                                   <  1:269107/183‑1 (MQ=11)
                                                                                          gCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg                                                                                                                                              >  1:436938/1‑201 (MQ=38)
                                                                                          gCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTg                                                                                                                                              <  2:436937/201‑1 (MQ=35)
                                                                                           cTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTCCCGATCAACAACCACTCTGAGCTGTAc                                                                                                                   >  1:559765/1‑227 (MQ=25)
                                                                                            gggCCAGATCAGCCTCGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCgg                                                                                                                           >  2:563307/2‑218 (MQ=11)
                                                                                              gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTCGACGGTCCCACCTTCGAGAACCTGCCACTGGGCGGCACCGCGAAGGTGGATGTGACCGACACCCGCGACGAGGTCGTGGCCAAGCTGACCGCCAccc                                                                                                                                                                                                            >  2:687328/1‑135 (MQ=11)
                                                                                              gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTc                                                                                                                                                <  1:377643/195‑1 (MQ=34)
                                                                                              gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGAc                                                                                                       >  1:757952/1‑235 (MQ=34)
                                                                                              gCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACACggc                                                                                                                          >  2:156594/1‑216 (MQ=21)
                                                                                              gCCAGATCAGCCTCGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAATAACCACTCGGAGCTGTACTTCAAGCTg                                                                                                         <  1:563308/233‑1 (MQ=11)
                                                                                                  gATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACg                                                                                                  <  1:432368/236‑1 (MQ=34)
                                                                                                           gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAggtg                                                                                                                                                                                                                                  >  2:651767/1‑100 (MQ=31)
                                                                                                           gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCa                                                                                                                                >  1:111302/1‑198 (MQ=33)
                                                                                                           gggCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCaccacc                                                                                          >  1:218798/1‑235 (MQ=33)
                                                                                                              aaTCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcg                                                                                      <  2:374085/235‑1 (MQ=21)
                                                                                                                 cACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTg                                                                                                                                                                <  2:138619/160‑1 (MQ=31)
                                                                                                                   cggccGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATcaa                                                                                                                                      <  2:135831/181‑1 (MQ=18)
                                                                                                                     tCCGCTGTGGATGTAGCCGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGGTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGa                                                                                                                                                                                               >  2:710836/1‑125 (MQ=17)
                                                                                                                     tCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGCGAGATCACCTACACCa                                                                                                                                                                        >  2:79860/1‑148 (MQ=17)
                                                                                                                               aTGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGcac                                                                                              >  2:430855/1‑211 (MQ=32)
                                                                                                                               aTGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTGACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACa                                                                       >  2:454537/1‑234 (MQ=255)
                                                                                                                                       ggTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACt                                                                                                                              <  2:96023/172‑1 (MQ=33)
                                                                                                                                            cacCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACcgtcgtc                                                                                    >  1:703457/1‑208 (MQ=35)
                                                                                                                                             cccTTCGAGAACCTGCACCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAc                                                                                                                                                                                                  <  2:208396/97‑1 (MQ=18)
                                                                                                                                             acCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCGTCACCCTGAc                                                                                                                                                              >  2:727491/1‑134 (MQ=17)
                                                                                                                                              ctttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAg                                                                                                                                                                                             <  1:279625/100‑1 (MQ=17)
                                                                                                                                              cgttCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCa                                                                                                                                                                                 >  2:115113/3‑114 (MQ=17)
                                                                                                                                               cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCAcc                                                                                                                                                                                                 <  2:403908/97‑1 (MQ=33)
                                                                                                                                               cTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc                                                                                                                                                                                <  1:115113/114‑1 (MQ=32)
                                                                                                                                                   gagaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGCCAGACACCCTCGACGAGGTGGTGGCGAAGCTGGCCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCa                                                                              <  1:216869/207‑1 (MQ=21)
                                                                                                                                                    agaACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACgg                                                                                                 >  2:237654/1‑187 (MQ=33)
                                                                                                                                                       aCCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAAcc                                             <  1:323371/236‑1 (MQ=38)
                                                                                                                                                          tGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAAcc                                                                                                                                 <  1:177119/150‑1 (MQ=34)
                                                                                                                                                          tGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAAcc                                                                                                                                 >  2:177118/1‑150 (MQ=34)
                                                                                                                                                              aCTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTc                                                                                                                             >  2:573812/1‑150 (MQ=34)
                                                                                                                                                                      gcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc                                                                                                                                                                   >  1:745813/1‑104 (MQ=33)
                                                                                                                                                                      gcAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCAcc                                                                                                                                                                   <  2:745812/104‑1 (MQ=33)
                                                                                                                                                                       cAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCg                                            <  2:757951/221‑1 (MQ=38)
                                                                                                                                                                               cGGTTCAAGTGACCGACACCCTCGACGAAGTCGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACt                                                                                                                              <  1:636021/132‑1 (MQ=25)
                                                                                                                                                                                       gTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCg                                       <  1:572653/210‑1 (MQ=255)
                                                                                                                                                                                          aCCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc                                                                                                                                                                                >  1:483691/1‑71 (MQ=35)
                                                                                                                                                                                          aCCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCAc                                                                                                                                                                                <  2:483690/71‑1 (MQ=35)
                                                                                                                                                                                             gACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATc                                                                                                                                                                      <  2:191267/78‑1 (MQ=34)
                                                                                                                                                                                               cacCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAAGGTCTGCCGATCAACAACCCCTCGGAGCTGTACTTCAAGCTGACCGa                                                                                                    >  2:244161/1‑141 (MQ=17)
                                                                                                                                                                                                  ccTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCa  >  1:366499/1‑236 (MQ=40)
                                                                                                                                                                                                    tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGCCCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGca                                                                    <  2:374705/169‑1 (MQ=255)
                                                                                                                                                                                                    tCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCCACCggcc                                         >  2:572652/1‑192 (MQ=255)
                                                                                                                                                                                                           |                                                                                                                                                                                                                                   
CTGAGCAACAACGCTCAGGTCACCATCAAGGCCGGTGAGACCAGTGCGCCGTACACCCACGACGCGCAAGGCGATGACGTCTACCAGGACGCTGGCCAGATCAGCCTGGGCATCACGTCCGCTGTGGATGTAGACGGTCACACCTTCGAGAACCTGCAACTGGGCGGCAACGCTTCGGTTCAAGTGACCGACACCCTCGACGAGGTGGTGGCGAAGCTGACCGCGACCCCTTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGACGGTCTGCCGATCAACAACCACTCGGAGCTGTACTTCAAGCTGACCGACGGCACCACCGTCGTCGTGGCAGCCAACAGCACCACCGGTTCGGCCACTGCAACCGCCCCGGACAACGTCTATGTCGGCACCAACGCGCCGGTGGTCA  >  AE015451/202073‑202502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: