| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 210,319 | C→G | 11.2% | T5275T (ACC→ACG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 210,319 | 0 | C | G | 11.2% | 124.9 / 4.0 | 42 | T5275T (ACC→ACG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (18/19); new base G (3/2); total (21/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACA > AE015451/210104‑210542 | acCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCt < 1:612272/236‑1 (MQ=255) gggTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAAcc < 1:245079/236‑1 (MQ=255) ggTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAAcc < 1:627795/235‑1 (MQ=255) tccAACGCCGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGTCGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCAccc < 2:618262/188‑1 (MQ=255) aCAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGa > 1:618263/1‑202 (MQ=255) aCAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCg > 2:535318/1‑212 (MQ=255) gCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaa < 1:710235/235‑1 (MQ=255) cTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACggg > 1:393301/1‑219 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCAcc > 1:321917/1‑149 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCAATCAACAACAACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGtcatc > 1:270618/1‑227 (MQ=255) cggTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTAACCGAAGGCGGCGAGATCACCTACACCATCACGCTGACCAACAAAGACGGTCTGCCGATCAACAAGca > 2:749114/1‑184 (MQ=255) gCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACgg > 1:427369/1‑236 (MQ=255) gCCACTGGTGCGACCTTCGAGAACCTGGAGCGGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACGGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGtca < 1:588388/217‑1 (MQ=255) tGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACgg > 2:513827/1‑231 (MQ=255) tGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTgg < 1:104813/236‑1 (MQ=255) cGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGcca > 2:308230/1‑154 (MQ=255) ttCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGt < 2:586389/227‑1 (MQ=255) ttCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc < 1:513828/236‑1 (MQ=255) ttCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGc > 1:236757/1‑236 (MQ=255) ttCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATc < 1:306259/153‑1 (MQ=1) ttCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTCCAAGTGACCGACACCACCGACGAAGTCGTGGCCAAGTTGACCGCCACCCCGTCGGTCACCGAAGGCGGCGAGATTACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATc > 2:306258/1‑153 (MQ=11) gaACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaa > 1:564764/1‑189 (MQ=255) gaACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCaa < 2:564763/189‑1 (MQ=255) gaACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCg < 1:189678/236‑1 (MQ=255) ccTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCg < 2:427368/233‑1 (MQ=255) ggTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCa > 1:438958/1‑196 (MQ=255) ggTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCa < 2:438957/196‑1 (MQ=255) ggTGCCGCTTCGGTCCAAGTGACCGACACCCTCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACa > 1:719701/1‑235 (MQ=255) cGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCa < 1:30437/153‑1 (MQ=38) ggCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCTACCGt > 2:302354/1‑216 (MQ=14) aaGTGACCGACACCCCCGACGAAGTGGTGGCCAAGCTGACTGCGCCCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCg < 1:195189/176‑1 (MQ=255) tGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTGACCGAAGGCGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAAcc < 1:354189/120‑1 (MQ=11) tGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAAcc > 2:354188/1‑120 (MQ=16) gACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTCCCTGCCCCGGACAACGTCTACGTc > 1:329290/1‑226 (MQ=17) gtggCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAATACGGCCTGCCGa > 2:245212/1‑88 (MQ=255) gCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAAAc > 1:451294/1‑131 (MQ=21) cAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAAcc > 2:316343/1‑92 (MQ=255) cAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAAcc < 1:316344/92‑1 (MQ=255) acTGACCGCCACCCCGTCGGTCACCGAAGGCGGAGAGATCACCTACACCATCCCCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAAc < 2:92092/147‑1 (MQ=255) gACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGTCCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTCACTGCCCCGGACAACGt > 2:423431/1‑180 (MQ=255) cGTCAGTCACCGAAGGCGGCGAGATCACCTACACCAGCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGc < 1:704024/82‑1 (MQ=255) gTCGGTCACCGAAGGCGGTGAGATCACCTACACCATCACGCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGGGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACg < 2:31329/179‑1 (MQ=255) cACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGt > 1:681213/1‑117 (MQ=37) acCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACa < 2:453583/232‑1 (MQ=18) | ACCCACGCTGCGCAGGGTGATGACGTTTACAACGACGCTGGCCAGATCAGCCTGGGCATCACCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTGGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGACACCACCGACGAAGTGGTGGCCAAGCTGACTGCGACCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACA > AE015451/210104‑210542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |