| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 210,465 | G→C | 14.0% | S5324T (AGC→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 210,465 | 0 | G | C | 14.0% | 103.7 / 5.0 | 34 | S5324T (AGC→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (20/9); new base C (2/3); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCAC > AE015451/210311‑210694 | acCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCAACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACa < 2:453583/232‑1 (MQ=18) caacaaAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCa < 2:719700/233‑1 (MQ=255) aaaGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGc < 2:236756/228‑1 (MQ=255) aGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGCGCAACTGACg > 2:588540/1‑205 (MQ=255) aGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAg > 2:265807/1‑225 (MQ=255) aGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGAGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTg > 2:667113/1‑208 (MQ=255) gCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGcaa > 2:563719/1‑157 (MQ=25) tGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAAc > 1:528596/1‑235 (MQ=255) cATTGACCTTCACGCTGAGCGACGGCAAAACCGTCATCACTGTGCCGGCCAACGGCACCGTGGGCACTGCCACCGTGACTGCTCCGGACAACGTCTACGTCGGCGCTAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGAc > 1:167798/1‑182 (MQ=11) tGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGc > 1:528310/1‑236 (MQ=255) tGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGACTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAg > 1:526849/1‑225 (MQ=255) ttCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGcc < 1:310727/182‑1 (MQ=255) cACGCTAAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTg < 1:730350/235‑1 (MQ=255) aCGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCAcc > 1:679475/1‑198 (MQ=255) aCGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCAcc < 2:679474/198‑1 (MQ=255) gCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCACGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTGCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAAc > 1:62505/1‑220 (MQ=255) tGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTAc > 2:80803/1‑208 (MQ=255) aCCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCGTCACCGcagaccagac > 1:720247/1‑236 (MQ=255) cGGCCAACGGCACCGTGGGCACTGCCACCGTGACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCAcc < 1:485630/218‑1 (MQ=255) cGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTg > 1:341094/1‑236 (MQ=255) ggCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGc > 2:311858/1‑170 (MQ=255) ggCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCaa < 2:341093/233‑1 (MQ=255) ggCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAAcc < 1:535319/236‑1 (MQ=255) gCACTGCCACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAAcc < 1:509196/235‑1 (MQ=255) gCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGTCGGTGCGGACGTCGGTACGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGCCGGTCACCGACGAGcc > 2:202748/1‑154 (MQ=255) aCTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCgc > 2:438298/1‑175 (MQ=255) ccGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCa > 1:316902/1‑234 (MQ=255) gCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGt > 1:217215/1‑236 (MQ=255) cccGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGt > 2:596842/1‑228 (MQ=255) cccGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGACGGTCACCGACGAGCCAGGCACCCCAGGCaa > 2:605577/1‑150 (MQ=255) tACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACTGACCTTCAc < 1:89238/212‑1 (MQ=255) tCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCgg > 1:588380/1‑180 (MQ=255) tCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGCCACCATCAcc > 1:75273/1‑164 (MQ=255) gcGGCACCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCAc < 1:211021/235‑1 (MQ=38) | ACCATCACCCTGACCAACAAAGACGGCCTGCCGATCAACAACCACAGTGCATTGACCTTCACGCTGAGCGACGGCAAGACCGTCATCACCGTGCCGGCCAACGGCACCGTGGGCACTGCAACCGTGACTGCCCCGGACAACGTCTACGTCGGCAGCAACGACCCTGTCGTGATGTCGATCGCTACCGTTGGCGGTGCGGACGTCGGTAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTCACCGACGAGCCAGGTACCCCAGGCAACGAAGGCGACCTGGTCAAGGTCACCATCACCGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACCTTCACTGTTCACGTCAACCAGCCGCTGGCTCAC > AE015451/210311‑210694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |