Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	3,951,159	T→C	100%	intergenic (‑123/+72)	PP_3486 ← / ← PP_3487	putative cytochrome c/conserved exported protein of unknown function

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	3,951,159	0	T	C	100.0%	121.7 / NA	35	intergenic (‑123/+72)	PP_3486/PP_3487	putative cytochrome c/conserved exported protein of unknown function
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/12);  total (23/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCC  >  AE015451/3950962‑3951383                                                                                                                                                                                                      |                                                                                                                                                                                                                                   cTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGc                                                                                                                                                                                               >  1:455884/1‑235 (MQ=255)                                                   ccagggcTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCCGGCGGCCCATGATACTCACCGCCCGGccc                                                                                                                                               >  2:665393/1‑232 (MQ=255)                                                         cTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGc                                                                                                                                                 >  2:579609/1‑225 (MQ=255)                                                              gTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGAc                                                                                                                                                                                                                           <  2:507110/146‑1 (MQ=255)                                                              gTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCgg                                                                                                                                           >  1:706105/1‑226 (MQ=255)                                                                    gTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACaaa                                                                                                                                                                                                        >  2:97873/1‑159 (MQ=255)                                                                    gTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGAGCTGCGCGCTACGTTCGCGACGCCAGGCCGCTCCCACAAAAGCGGATGcc                                                                                                                                                                                              >  1:48634/1‑169 (MQ=39)                                                                     ttGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGcc                                                                                                                                                                                                                >  2:610190/1‑149 (MQ=255)                                                                                     gCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCCAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGAt                                                                                                                                                                                                 >  2:603171/1‑149 (MQ=255)                                                                                               cATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGTGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGcc                                                                                                                                                                                     >  1:298605/1‑151 (MQ=255)                                                                                               cATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGTGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGcc                                                                                                                                                                                     <  2:298604/151‑1 (MQ=255)                                                                                               cATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAg                                                                                                                                              >  2:355877/1‑190 (MQ=255)                                                                                                   tCGCAAGCCGTGTGGGAGCGGCCTTGCGGCGCGAAAGGGCTGCGCGGCAGCCCCGGCAGTGGAAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGcc                                                                                                                                                                     <  1:614985/163‑1 (MQ=38)                                                                                                      cAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCg                                                                                       >  1:466692/1‑236 (MQ=255)                                                                                                                 ggAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGGGGCAGCTCAAGCGCAGAAAGAGTGGAGCGCTGCGCGCCCCTTTCGCGa                                                                                                                                                                                                                            >  2:75633/1‑94 (MQ=37)                                                                                                                       gCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAg                                                                      <  1:97873/236‑1 (MQ=255)                                                                                                                        ccTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCcaca                                                                                                                                                                                           >  2:691851/1‑120 (MQ=255)                                                                                                                          ttGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCACGa                                                                                                                                                                >  2:80857/1‑145 (MQ=255)                                                                                                                                cgcgAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATg                                                                                                                                                                 >  2:693918/1‑138 (MQ=255)                                                                                                                                               ccgcagcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCcaca                                                                                                                                                                                                          <  2:117993/80‑1 (MQ=255)                                                                                                                                                agccgcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGTCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCg                                                                                       >  2:392423/1‑194 (MQ=255)                                                                                                                                                agcagcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGc                                                                                                                              <  2:243606/157‑1 (MQ=255)                                                                                                                                                agcagcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGc                                                                                                                              >  1:243607/1‑157 (MQ=255)                                                                                                                                                agcagcCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGc                                               >  1:578939/1‑234 (MQ=255)                                                                                                                                                     ccccGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTg                                                                            >  1:502350/1‑200 (MQ=255)                                                                                                                                                                     tCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCa                                                                                                                                                                   <  1:226423/99‑1 (MQ=255)                                                                                                                                                                       aaGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCaccgaccg                                                                                                                                                   >  2:291333/1‑113 (MQ=255)                                                                                                                                                                        aGCGCAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCg                      <  1:355878/235‑1 (MQ=255)                                                                                                                                                                           gcAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCa                                                    <  1:674575/202‑1 (MQ=255)                                                                                                                                                                           gcAGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCa                                                    >  2:674574/1‑202 (MQ=255)                                                                                                                                                                             aGAAAGAGGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTc                <  1:179439/236‑1 (MQ=255)                                                                                                                                                                                 agagGGGAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGC‑GATTGCCCAGC‑GCATGCAGCTGTTGACCCAGCCGCTGGCGGTGCATCCGGGCGaa                                                                                 >  2:121336/1‑167 (MQ=21)                                                                                                                                                                                       gAGCGCTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACt      <  1:665394/236‑1 (MQ=255)                                                                                                                                                                                          cgcTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGc                                                                     <  1:535179/170‑1 (MQ=255)                                                                                                                                                                                            cTGCGCGCTCCTTTCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCCGCTGCGTCGAATGCTGCTCGGGACTCGGCCGGAACTGccc  >  1:105177/1‑235 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                                                   CTCGCGGTAAAGGCGTTTGCCTGCTTGTTCGTGGTCGGTCAGGTCCAGGGCCAGGGCTGAGGTCGAAGTTGCCATGCCAAGCACGCAGGTAAGCCATATCGCAAGCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCTGCGCAGCAGCCCCGGCAGTGGCAGCTCAAGCGCAGAAAGAGGGGAGCGCTGCGCGCTCTTATCGCGACGCAAGGCCGCTCCCACAAAAGCGGATGCCACAGAGGCCAATTCCCCAGGCGGCCCATGATACTCACCGACCGGCCAGCGGCTGGGTCAACAGCTGCATGC‑GCTGGGCAATC‑GCCGCTGGCGGTGCATCCGGGCGAATCTTGCTCCAGCGTTTACCTGCAACATCACCGGCAATCAGCTGCGTCGAATGCTGCTCGGGACTCGGCAGGAACTGCCC  >  AE015451/3950962‑3951383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑