| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AE015451 | 1,499,498 | 1 bp→CG | 100% | intergenic (+75/‑89) | trpS → / → zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451 | 1,499,497 | 1 | . | C | 81.3% | 86.6 / 17.3 | 32 | intergenic (+74/‑90) | trpS/zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base . (1/5); new base C (8/18); total (9/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | AE015451 | 1,499,498 | 0 | T | G | 84.4% | 79.2 / 11.1 | 32 | intergenic (+75/‑89) | trpS/zapE | Tryptophan‑‑tRNA ligase/putative enzyme with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (9/18); minor base C (0/5); total (9/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCC‑GGTGTT‑T‑CGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCA > AE015451/1499294‑1499731 ||| cGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGc > 1:685975/1‑236 (MQ=255) gCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCAtt < 1:419712/235‑1 (MQ=255) cTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTaa < 1:259222/235‑1 (MQ=38) aGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCAcg‑c < 2:258763/177‑1 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTT‑CGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGc < 1:312801/228‑1 (MQ=38) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:242759/235‑1 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTT‑CGCGGGCACGCCCGCTCCCACAGACTGATTGCCAtt < 2:251412/179‑1 (MQ=37) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTT‑CGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCggg < 2:624179/185‑1 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTg > 2:637239/1‑166 (MQ=25) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTg < 1:637240/166‑1 (MQ=25) aaGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:680010/225‑1 (MQ=38) ccGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTT‑CGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 2:685974/234‑1 (MQ=255) cGTGATGCGGCCATGCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGAc < 1:147606/188‑1 (MQ=37) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCCTTGCCGGGGGTC‑G‑CGGGCAcg‑ccc > 2:532211/1‑137 (MQ=25) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg > 1:669361/1‑139 (MQ=21) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 2:669360/139‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg < 2:322714/234‑1 (MQ=255) cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:753791/178‑1 (MQ=37) cagcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTT‑CGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:685326/178‑1 (MQ=37) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:481403/177‑1 (MQ=37) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATTACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc > 1:587875/1‑212 (MQ=38) cagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcgcg < 1:455608/234‑1 (MQ=255) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAATTCTTCCATGACGCGGCGCAGGAAAc > 1:636183/1‑234 (MQ=38) gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCg > 1:324463/1‑236 (MQ=255) ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTa < 2:106464/100‑1 (MQ=11) ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCAcc < 1:333864/235‑1 (MQ=255) gCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTACAACGCTATCAAGCa < 1:317613/174‑1 (MQ=37) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCATAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGATGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCAc < 2:387174/229‑1 (MQ=39) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTACGCGGGc > 2:108763/1‑83 (MQ=14) cAGGCGCCGCGGTGCATGGCACCGGCTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 2:503605/143‑1 (MQ=37) cTTTGCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCg > 1:588017/1‑236 (MQ=255) tggCC‑GGTGTTCG‑CGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt < 2:244646/111‑1 (MQ=38) cgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGCGCATGTTCGGCa > 1:243148/3‑236 (MQ=255) ||| CGAAGGCCTGAGCTTCGGCCTGTGGCTCAATGGCGAGCAGGTTGCCGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCC‑GGTGTT‑T‑CGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCGCAGGAAACTGCGGTGCGTCACCTCCAGCGCCTGTACGACGACCTGGTGCACGCGCAGAACAACAAGCCGGGCATGTTCGGCA > AE015451/1499294‑1499731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |